İneklerde Komplikasyonlu Gebeliklerde Serum Haptoglobin Düzeyleri

Abstract

Bu çalışma komplikasyonlu gebe ineklerin gebelik dönemlerinde serum haptoglobin düzeylerinin belirlenmesi amacıyla yapılmıştır. Çalışmaya aynı işletmeye ait doğum süreçleri takip edilen, sağlıklı doğum gerçekleştiren 14 inek ve komplikasyonlu gebeliğe sahip olan 14 inek olmak üzere toplam 28 hayvan dahil edilmiştir. Kan örnekleri gebeliklerinin 6. ayında vena jugularisten sarı kapaklı biyokimya tüplerine alınmıştır. Serum albümin, globulin ve total protein ölçümleri otoanalizör cihazı ile yapılmıştır. Haptoglobin seviyelerinin belirlenmesinde ticari test kiti kullanarak ELISA cihazında ölçülmüştür. Serum haptoglobin seviyeleri komplikasyonlu gebelerde 226.64±24.09 µg/ml; sağlıklı gebelerde ise 46.71±2.98 µg/ml olarak tespit edilmiştir. Komplikasyonlu ve sağlıklı gebelerde sırasıyla total protein 8,52±0,28-7,58±0,12 g/dL; albümin 3,31±0,08-3,34±0,04 g/dL; globulin 5,21±0,24-4,25±0,15 g/dL; alb/glb 0,65±0,03-0,80±0,04 olarak bulunmuştur. Serum haptoglobin, total protein, globulin ve alb/glb değerleri kontrol grubuna göre komplikasyonlu gebelik grubunda istatiksel olarak anlamlı görülmüştür (p<0.05).

Keywords

• Komplikasyonlu gebelik
• inek
• haptoglobin
• Complicated pregnancy
• cow

Serum Haptoglobin Levels in Complicated Pregnancies in Cows

Abstract

This study was carried out to determine serum haptoglobin levels of pregnant cows with complications during pregnancy. A total of 28 animals were included in the study, 14 cows with healthy birth and 14 cows with complicated pregnancies, whose birth processes were followed in the same farm. Blood samples were taken from the vena jugularis into yellow capped biochemistry tubes at the 6th month of their pregnancy. Serum albumin, globulin and total protein measurements were made with an autoanalyzer. Determination of haptoglobin levels was measured in an ELISA device using a commercial test kit. Serum haptoglobin levels were found as 226.64±24.09 µg/ml in pregnant cows with complications and 46.71±2.98 µg/ml in healthy pregnant cows. Total protein 8.52±0.28-7.58±0.12 g/dL, respectively; albumin 3.31±0.08-3.34±0.04 g/dL; globulin 5.21±0.24-4.25±0.15 g/dL; alb/glb 0.65±0.03-0.80±0.04 was found to be in complicated and healthy pregnant. Serum haptoglobin, total protein, globulin and alb/glb values were statistically significant in the complicated pregnancy group compared to the control group (p<0.05).

Keywords

• Complicated pregnancy
• cow
• haptoglobin

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