Comparison Of The Effects Of Andız (Juniperus sp) Root Extract And Vitamin B12 On Nerve Healing in Rats With Sciatic Nerve Injury

Abstract

The self-repair capacity of peripheral nerves is limited, and the repair of severely damaged or significantly flawed nerves is challenging. For preclinical investigations on peripheral nerve regeneration, the sciatic nerve injury model is the most often used experimental model. In rats, sciatic nerve injury is the most frequently used model in studies related to peripheral nerve regeneration. Material and Methods: In this study, 32 rats were subjected to sciatic nerve injury, and applications of B12 vitamin and Andız root extract were performed. After the applications, the rats were sacrificed on the 21st day, and samples of the sciatic nerve tissue were taken. The collected samples were examined using histopathological and immunofluorescence methods. Results: The examination revealed that B12 vitamin and Andız root extract, when applied separately to the rats, provided limited and similar benefits histopathologically, while the group that received combined treatment showed more effective positive effects compared to all other groups. Additionally, when assessing S100 and GFAP expression through immunofluorescence, it was observed that individual applications provided similar benefits, while the group that received combined treatment contributed more effectively compared to all other groups. Conclusion: In conclusion, in the rat model of sciatic nerve injury, it was determined that Andız root extract and B12 vitamin, when applied separately, had limited and similar effects compared to the control group in determining the levels of recovery. However, when applied together, they exhibited a synergistic effect, providing a greater contribution to nerve regeneration compared to all other groups.

Keywords

• Andız root extract
• B12 vitamin
• injury
• nerve regeneration
• sciatic nerve
• treatment.

Siyatik sinir yaralanması oluşturulan ratlarda Andız (Juniperus sp) kökü ekstraktının ve B12 vitamininin sinir iyileşmesi üzerine etkisinin karşılaştırılması

Abstract

Periferik sinirlerin kendi kendini onarma kapasitesi sınırlıdır ve ciddi şekilde hasar görmüş veya önemli kusurları olanların onarımı zordur. Bu nedenle periferik sinir rejenerasyonu ile ilgili klinik öncesi çalışmalar için en sık kullanılan deneysel model, siyatik sinir yaralanması modelidir. Ratlarda siyatik sinir yaralanması, periferik sinir rejenerasyonu ile ilgili çalışmalarda en çok kullanılan modeldir. Materyal Metot: Bu çalışmada 32 rata siyatik sinir yaralanması sonrası B12 vitamini ve Andız kökü ekstraktı uygulamaları yapılmıştır. Uygulamalar sonrasında ratlar 21. günde sakrifiye edilerek siyatik sinir doku örnekleri alınmıştır. Alınan örnekler histopatolojik ve immunofloresan yöntemlerle incelenmiştir. Bulgular: İncelenme B12 ve Andız kökü ekstraktının ayrı ayrı uygulandığı ratlarda histopatolojik olarak sınırlı düzeyde ve benzer şekilde fayda sağladığı, birlikte uygulanan grubun ise tüm gruplara göre daha etkili pozitif yönde etki sağladığı görülmüştür. Ayrıca immunofloresan yöntemle S100 ve GFAP ekspresyonunda da ayrı ayrı uygulamaların benzer şekilde fayda sağladığı görülürken, birlikte uygulama yapılan grupta ise diğer tüm gruplara kıyasla daha iyi katkı sağladığı görülmüştür. Sonuç: Sonuç olarak ratlarda uygulanan siyatik sinir yaralanması modelinde iyileşme düzeylerinin belirlenmesi için ayrı ayrı uygulanan Andız kökü ekstraktı ve B12 vitaminin kontrol grubuna göre sınırlı düzeyde ve benzer şekilde etki oluşturduğu, birlikte uygulandığında ise sinerjik etki göstererek diğer tüm gruplara göre sinir iyileşmesine daha etkili bir şekilde katkı sağladığı belirlenmiştir.

Keywords

• Andız kökü ekstraktı
• B12 vitamini
• sinir rejenerasyonu
• siyatik sinir
• tedavi.

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