Breast Cancer Stem Cells and Iron Dependency

Yıl 2018, Cilt: 45 Sayı: 2, 195 - 200, 15.06.2018

Ufuk Ozer

https://doi.org/10.5798/dicletip.419307

Öz

Objectives:
Among woman, breast cancer is the most prevalent cancer worldwide. It is
composed of various cell types that are classified into different subtypes such
as triple negative breast cancer (TNBC), triple positive breast cancer (TPBC).
TNBCs and TPBCs represent distinct genetic background, thereby leading to
therapeutic diversity in breast cancer. It is critical to know their
tumorigenic properties to overcome the diversity.

Methods:
TPBC cell lines; BT474, HCC1954 and TNBC cell lines; MDA-MB-435, Hs578T,
MDA-MB-231 were used to measure intracellular iron levels via a fluorescent
probe, calcein-AM, utilizing flow cytometry. Breast cancer stem cells (BCSCs)
are detected by surface expression of CD44+/CD24- markers in the lines.

Results:
Here, it has shown that TNBCs have higher basal levels of iron and population
of BCSCs than TPBCs. Iron addition provides enrichment of BCSCs in TPBCs.

Conclusion:
Iron is an important element for maintenance of BCSCs. 

Anahtar Kelimeler

Breast cancer stem cells, iron, TNBC

Kaynakça

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