EFFECTS OF PULSING ELECTROMAGNETIC FIELD ON THE GROWTH OF SACCHAROMYCES CEREVISIAE

Yıl 2005, Sayı: 009, 55 - 64, 15.12.2005

E. Gülbandılar

Öz

In this study, the effects of 15 Hz-pulsing electromagnetic field (PEMF) on the growth of Saccharomyces cerevisiae yeast have been investigated. After statistical assessments, there was no significant difference between control and experimental group on the base of adaptation (first 6 hours of incubation period) and static phase (after 26 hours) (P>0.05). After inoculation (between 6 and about 26 hours), count of the cells growing under the effect of the magnetic field was lower than that of control group, and the results were statistically significant (P<0.001). In addition, growth curves of experimental and control group were mathematically studied and growth equations were formed. After statistical measurements a considerable accordance was found to be between experimental and theoretical growth curves (rC=0.998, rPEMF=0.99).

Anahtar Kelimeler

Electromagnetic field, growth curve, yeast

SACCHAROMYCES CEREVISIAE MAYASININ ÜREMESİNE PULSLU ELEKTROMANYETİK ALANIN ETKİSİ

Öz

Bu çalışmada, Saccharomyces cerevisiae maya hücrelerinin büyümesine 15 Hz’li pulslu elektromanyetik alanın etkisi araştırılmıştır. İstatistiksel incelemeler sonunda uyum (inkübasyon periyodunun ilk 6 saati) ve durgun (16-29 saatler arası) döneminde deney ve kontrol grupları arasında anlamlı fark bulunamamıştır (P>0.05). Ekimden sonraki 6. saat ile 26. saat arasında, manyetik alanın etkisinde büyüyen hücrelerin sayısının kontrol grubunun hücre sayısından daha düşük olduğu ve sonuçların istatistiksel olarak anlamlı olduğu bulunmuştur (P<0.001). Ayrıca deney ve kontrol gruplarının büyüme eğrileri matematiksel olarak incelenip, büyüme denklemleri oluşturulmuştur. İstatistiksel incelemeler sonucunda, deneysel ve teorik büyüme eğrileri arasında bir korelâsyonun olduğu bulunmuştur (rC=0.998, rPEMF=0.99).

Anahtar Kelimeler

Elektromanyetik alan büyüme eğrisi, Maya

Kaynakça

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