Sıçanlarda Kombine Metformin ve Egzersizin Anti-inflamatuar, Antioksidatif ve Kardiyoprotektif Etkilerinin Araştırılması

Abstract

Amaç: Bu çalışmanın amacı egzersiz ve metformin tedavisinin tek başına veya birlikte uygulanmasının anti-enflamatuar, antioksidatif ve kardiyoprotektif etkilerinin araştırılmasıdır. Gereç ve Yöntemler: Bu çalışmada 42 adet erkek Wistar sıçan kullanılmıştır. Sıçanlar kontrol (CONT), egzersiz (EXE), 100 mg/kg metformin (M100), 200 mg/kg metformin (M200), 100 mg/kg metformin+egzersiz (M100+EXE) ve 200 mg/kg metformin+egzersiz (M200+EXE) olmak üzere altı gruba ayrılmıştır. Egzersiz, egzersiz eğitimi de dahil olmak üzere 10 hafta boyunca uygulanmıştır. Metformin egzersizden 30 dakika önce uygulanmıştır. Çalışma sonunda sıçanlardan alınan serum örneklerinde, C-reaktif protein (CRP), tümör nekroz faktörü-alfa (TNF-α), interlökin-6 (IL-6), interlökin-10 (IL-10), kardiyak troponin-I (cTn-I), kreatin kinaz-kas/beyin (CK-MB), katalaz (CAT), süperoksit dismutaz (SOD), glutatyon peroksidaz (GPx) ve malondialdehit (MDA) ELISA yöntemi kullanılarak ölçülmüştür. Bulgular: Metformin ve egzersizin birlikte uygulanması cTn-I, CK-MB, MDA, TNF-α, CRP ve IL-6 düzeylerini anlamlı şekilde azaltmıştır (p<0,001). Buna karşılık, SOD, CAT, GPx ve IL-10 düzeylerini de önemli şekilde artırmıştır (p<0,001). Tek başına veya kombinasyon halinde tedavi edilen grupların glukoz seviyeleri CONT grubundan istatistiksel olarak anlamlı şekilde daha düşük bulunmuştur (p<0,001). Sonuç: Bu çalışmanın bulguları, hem metformin hem de egzersiz uygulamasının, tek başına veya kombinasyon halinde, Wistar sıçanlarında önemli anti-inflamatuar, antioksidan ve kardiyoprotektif etkiler gösterdiğini ortaya koymaktadır. Bu sonuçlar, metformin tedavisinin düzenli egzersizle birleştirilmesinin, kardiyovasküler risk faktörlerini azaltmak ve antioksidan savunmaları güçlendirmek için sinerjik bir yaklaşım sunabileceğini düşündürmektedir.

Keywords

• Uzun ömür
• kardiyovasküler hastalıklar
• hiperglisemi
• oksidatif stress
• metformin
• egzersiz

Investigation of Anti-inflammatory, Antioxidative, and Cardioprotective Effects of Combined Metformin and Exercise in Rats

Abstract

Aim: This study aimed to investigate the anti-inflammatory, antioxidative and cardioprotective effects of exercise and metformin treatment applied alone or in combination. Material and Methods: In this study, 42 male Wistar rats were used. The rats were separated into six groups as control (CONT), exercise (EXE), 100 mg/kg metformin (M100), 200 mg/kg metformin (M200), 100 mg/kg metformin+exercise (M100+EXE), and 200 mg/kg metformin+exercise (M200+EXE). Exercise was applied for 10 weeks including exercise training. Metformin was administered 30 minutes before exercise. At the end of the study, levels of C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6), interleukin-10 (IL-10), cardiac troponin-I (cTn-I), creatine kinase-muscle/brain (CK-MB), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx) and malondialdehyde (MDA) in serum samples from rats were quantified using the ELISA method. Results: The combined application of metformin and exercise significantly decreased cTn-I, CK-MB, MDA, TNF-α, CRP and IL-6 levels (p<0.001). In contrast, it increased SOD, CAT, GPx, and IL-10 levels significantly (p<0.001). Glucose levels of groups treated alone or in combination were found statistically significantly less than CONT group (p<0.001). Conclusion: The findings of this study reveal that both metformin and exercise administration, alone or in combination, exert significant anti-inflammatory, antioxidant, and cardioprotective effects in Wistar rats. These results suggest that combining metformin therapy with regular exercise may offer a synergistic approach to reducing cardiovascular risk factors and enhancing antioxidant defenses.

Keywords

• Longevity
• ardiovascular diseases
• hyperglycemia
• oxidative stress
• metformin
• exercise

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