Histoultramicroscopic Investigation of the Rats’ Thymus (Experimental Data)

Year 2021, Volume: 23 Issue: 2, 142 - 150, 30.08.2021

Olha Prykhodko , Serhii Dmytruk , Olga Avilova , Eliska Kubikova , Valentyna Bumeister , Olha Yarmolenko , Olena Gordienko

https://doi.org/10.18678/dtfd.913381

Cited By: 2

Abstract

Aim: The research paper presents the characteristic of cytoarchitectonics of the thymus of intact white mature male laboratory rats. Topicality of the study is due to the need to clarify the data on the contribution of each type of thymus cells in the formation of its structure. The aim of the research was to determine the specifics of localization and ultramicroscopic structure of thymus cells in male mature Wistar laboratory rats.
Material and Methods: The study was conducted using histological and ultramicroscopic methods on 10 mature male laboratory rats, weighing 130-150 g. Semi-thin (0.5-1 μm) and ultrathin (0.05-0.2 μm) sections were made on a microtome UMTP-4 (Ukraine), which were stained with 1% methylene blue solution with the addition of 1% sodium tetraborate solution. Histological analysis and photographic recording were performed using Olympus light microscope (Japan) and DSM 510 camcorder with magnification in 1000 times.
Results: With a detailed study of the semi-thin and ultrathin sections in the thymus lobules the specifics of localization and ultramicroscopic structure of thymus cells were clearly identified. The features of localization and ultramicroscopic structure of epithelial, mesenchymal, vascular and hematopoietic thymus cells were determined from the point of view of their functional loads and interactions.
Conclusion: The described structural peculiarities of the components of the thymus and their relative location in different zones reflect significant organ polymorphism, which must be taken into account in order to achieve the required level of objectivity in the result evaluation of simulated biomedical experiments.

Keywords

Thymus gland, Rats, histology, ultrastructure, Experiment

Supporting Institution

Sumy State University, Sumy, Ukraine

Project Number

NA20328K

Sıçanların Timusunun Histoultramikroskopik İncelenmesi (Deneysel Veri)

Abstract

Amaç: Bu araştırma makalesi, sağlam beyaz olgun erkek laboratuvar sıçanlarının timusunun sitoarkitektonik özellikleri hakkında bilgi sunmaktadır. Çalışmanın güncelliği, her bir timus hücresi tipinin timüs yapısının oluşumuna olan katkısı hakkındaki verilerin net bir şekilde ortaya çıkarılmasına olan ihtiyaçtan kaynaklanmaktadır. Bu araştırmanın amacı, erkek olgun Wistar laboratuvar sıçanlarında timus hücrelerinin lokalizasyon ve ultramikroskopik yapısının özelliklerini belirlemektir.
Gereç ve Yöntemler: Bu çalışma, 130-150 g ağırlığındaki 10 olgun erkek laboratuvar sıçanı üzerinde histolojik ve ultramikroskopik yöntemler kullanılarak yapıldı. Bir UMTP-4 (Ukrayna) mikrotomu ile yarı ince (0.5-1 μm) kesitler ve ultra ince (0.05-0.2 μm) kesitler yapıldı, yapılan bu kesitler %1 sodyum tetraborat solüsyonu ilave edilerek %1 metilen mavisi solüsyonu ile boyandı. Histolojik analiz ve fotoğraf kaydı Olympus ışık mikroskobu (Japonya) ve DSM 510 video kamera kullanılarak 1000 kez büyütme ile yapıldı.
Bulgular: Timus lobüllerindeki yarı-ince kesitler ve ultra-ince kesitlerin detaylı bir şekilde incelenmesi ile timus hücrelerinin lokalizasyon ve ultramikroskobik yapısının özellikleri net bir şekilde ortaya çıkarıldı. Epitelyal, mezenkimal, vasküler ve hematopoetik timus hücrelerinin lokalizasyon ve ultramikroskopik yapısının özellikleri, bu hücrelerin fonksiyonel yükleri ve etkileşimleri açısından dikkate alınarak belirlendi.
Sonuç: Timusun bileşenlerinin tanımlanmış yapısal özellikleri ve farklı bölgelerdeki göreceli konumları, simüle edilmiş biyomedikal deneylerin sonuçlarının değerlendirilmesinde gerekli olan ve istenen nesnellik düzeyinin elde edilebilmesi için dikkate alınması gereken önemli organ polimorfizmini yansıtır.

Keywords

Timus bezi, sıçanlar, histoloji, ultrastrüktür, Deney

Project Number

NA20328K

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