Pitavastatinin Ratlarda Termal Plantar ve Dinamik Plantar Testler ile Ağrı Yanıtına Etkinliğinin Değerlendirilmesi

Year 2024, Issue: Early Access

Selma Yaman , Duygun Altıntaş Aykan , Tuba Ozcan Metin

https://doi.org/10.18678/dtfd.1551657

Abstract

Amaç: Pitavastatin (Pita), iyi bilinen bir hipolipidemik ajan olan statin ailesinin bir üyesidir ve bazı statin üyelerinin ağrı tedavisinde etkili olduğu gösterilmiştir. Bu çalışmada sıçanlarda farklı doz ve sürelerde nosisepsiyon ölçülerek Pita'nın antinosiseptif etkilerinin değerlendirilmesi amaçlanmıştır.
Gereç ve Yöntemler: Bu çalışma ilaçsız kontrol olarak salin oral, tek doz 1 mg/kg Pita, tek doz 3 mg/kg Pita, 14 gün boyunca 1 mg/kg Pita, 14 gün boyunca 3 mg/kg Pita ve 14 gün boyunca 20 mg/kg diklofenak olmak üzere altı gruptan oluşmaktadır. Ağrı eşiği değişikliklerini gözlemlemek için termal plantar ve mekanik plantar testler kullanıldı. Pita dozları, süreleri ve davranışsal ağrı tepkileri arasındaki korelasyonlar değerlendirildi. Siyatik sinirler histopatolojik olarak değerlendirildi.
Bulgular: Pita, 14 gün boyunca uygulanan 1 mg/kg dozunda mekanik eşiği 25,43±4,79 g'dan 32,28±2,27 g'a çıkararak (p=0,041) ve 14 gün boyunca uygulanan 3 mg/kg dozunda ise mekanik eşiği 27,41±2,36 g'dan 34,35±2,58 g'a çıkararak (p=0,039) dinamik plantar testte anlamlı bir antinosiseptif etki gösterdi. Ayrıca Pita, termal plantar testte 3 mg/kg tek dozda termal latansı 8,95±1,28 sn'den 11,71±1,49 sn'ye çıkardı (p=0,004). Dinamik plantar ve termal plantar testlerde Pita'nın antinosiseptif etkileri kanıtlanmış olmakla birlikte, bu bulgular histopatolojik değerlendirmede anlamlı bir düzeye ulaşmadı.
Sonuç: Bu sonuçlar Pita'nın antinosiseptif etkiye sahip olduğunu ve uygun doz ve zamanlama ile kullanıldığında ağrı tedavisinde tercih edilebileceğini düşündürmektedir.

Keywords

Ağrı, Pitavastatin, Antiallodinik Etki, Antihyperaljesik Etki

Evaluation of The Efficacy of Pitavastatin on Pain Response in Rats with Thermal Plantar and Dynamic Plantar Tests

Abstract

Aim: Pitavastatin (Pita) is a member of the statin family, a well-known hypolipidemic agent, and some statin members are effective in treating pain. This study aimed to evaluate the antinociceptive effects of Pita by measuring nociception in rats with different doses and durations.
Material and Methods: This study consisted of six groups including saline oral as a non-drug control, 1 mg/kg Pita single dose, 3 mg/kg Pita single dose, 1 mg/kg Pita for 14 days, 3 mg/kg Pita for 14 days, and 20 mg/kg diclofenac for 14 days. Thermal plantar and mechanical plantar tests were used to observe pain threshold changes. Correlations between Pita doses, durations, and behavioral pain responses were evaluated. The sciatic nerves were evaluated histopathologically.
Results: Pita showed a significant antinociceptive effect in the dynamic plantar test at doses of 1 mg/kg for 14 days by increasing the mechanical threshold from 25.43±4.79 g to 32.28±2.27 g (p=0.041) and 3 mg/kg for 14 days by increasing the mechanical threshold from 27.41±2.36 g to 34.35±2.58 g (p=0.039). Also, Pita increased the thermal latency from 8.95±1.28 s to 11.71±1.49 s in the thermal plantar test at a single dose of 3 mg/kg (p=0.004). Although the antinociceptive effects of Pita were proven in dynamic plantar and thermal plantar tests, these findings did not reach a significant level at histopathological evaluation.
Conclusion: These results suggest that Pita has an antinociceptive effect and when used in conjunction with the right dosage and timing, might be favored for the treatment of pain.

Keywords

Pitavastatin, Antialoodynic Effect, Antihyperalgesic Effect, Pain

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