Comparison of Antibacterial Activity of New Generation Self-Etching Adhesive Systems

Yıl 2009, Cilt: 36 Sayı: 1 - Cilt: 36 Sayı: 1, 7 - 13, 01.02.2009

Aylin Akbay Oba Işıl Şaroğlu Sönmez Jülide Sedef Göçmen Merve Erkmen Murat Yıldırım

Öz

The aim of this study was to evaluate theantibacterial effects of different self-etchingprimer/adhesives on Streptococcus mutans S.mutans , Lactobacillus acidophilus L. acidophilus and Lactobacillus casei L. casei . The antibacterial effects of Clearfil ProtectBond Primer, Adper Easy Bond and Xeno V weretested against standart strains of S. mutans, L. caseiand L. acidophilus using the disk diffusion method.Chlorhex was used as a positive control material,consisting of 2 % chlorhexidine. Standart filterpaper disks n=6 impregnated with 20 µl of eachmaterial were prepared. After incubation at 37°Cfor 48 hours, zones of inhibited bacterial growthwere measured in milimeters. Data were analyzedusing ANOVA, Friedman test and Kruskal-Wallistest. The difference between antibacterial effect ofthe tested material groups were statistically significant for each bacterial strains p < 0,05 . Theresults indicated that Clearfil Protect Bond Primerhad the strongest effect against the S. mutans andL. casei, and Xeno V produced the largest inhibition zone for L. acidophilus among the test materials. Clearfil Protect Bond Primer was showed anantibacterial effect closest to chlorhexidine for S.Mutans and L. Casei Clearfil Protect Bond and Xeno V showed different levels of antibacterial effect.

Anahtar Kelimeler

Dentin-Bonding Agents, Streptococcus mutans, Disk Diffusion Antimicrobial Tests

Yeni nesil self-etching adeziv sistemlerin antibakteriyel özelliklerinin karşılaştırılması

Öz

Bu çalışmanın amacı farklı self-etching adezivsistemlerin antibakteriyel etkilerinin, Streptococcus mutans S. mutans , Lactobacillus acidophilus L. acidophilus ve Lactobacillus casei L. casei bakterileri üzerinde değerlendirilmesidir.Clearfil Protect Bond Primer, Adper EasyBond ve Xeno V self-etching adeziv materyallerininantibakteriyel etkileri, S. mutans, L. casei and L.acidophilus standart suşlarında disk difüzyon yöntemi kullanılarak test edilmiştir. % 2’lik klorheksidin içeren Klorhex pozitif kontrol materyaliolarak kullanılmıştır. Her materyalden 20 µlemdirilmiş steril kağıt diskler n=6 hazırlanmış,37°C de 48 saat inkübasyondan sonra diskler etrafında oluşan inhibisyon zonları milimetre olarakölçülmüştür. Sonuçların istatistiksel değerlendirmeleri ANOVA, Friedman ve Kruskal-Wallis testleri kullanılarak yapılmıştır.Test edilen materyal gruplarının antibakteriyel etkinlikleri arasındaki fark, her bakteri suşuiçin istatistiksel olarak anlamlı bulunmuştur p

Anahtar Kelimeler

Dentin-Bonding Ajanlar, Streptococcus mutans, Disk Difüzyon Antimikrobiyal Testleri

Kaynakça

• Gondim JO, Duque C, Hebling J, Giro EMA. Influence of human dentine on the antibacterial activity of self-etching adhesive systems against ca- riogenic bacteria. J Dent 2008; 241-8.
• Schmalz G, Ergucu Z, Hiller KA. Effect of dentin on the antibacterial activity of dentin bonding agents. J Endod 2004;30:352-8.
• Yazıcı R, Ozalp M, Baseren M, Dayangac B. İki farklı amalgam bonding primerinin antibakteriyel aktivitelerinin değerlendirilmesi. CÜ Diş Hek Fak Derg 2002; 5:85-7.
• Fusayama T. Two layers of carious dentin: diagnosis and treatment. Oper Dent 1979; 4: 63-70.
• Atac AS, Cehreli ZC, Sener B. Antibacterial activity of fifth-generation dentin bonding systems. J Endod 2001;27:730-3.
• Gao W, Smales RJ, Yip HK. Deminerali- sation and remineralisation of dentine caries, and the role of glass-ionomer cements. Int Dent J 2000; 50:51-6.
• Meiers JC, Miller GA. Antibacterial activity of dentin bonding systems, resin-modified glass ionomers, and polyacid-modified composite resins. Oper Dent 1996; 21:257-64
• Atac AS, Cehreli ZC, Sener B. Antibacterial activity of fifthgeneration dentin bonding systems. J Endod 2001;27:730–3.
• Imazato S, Kuramoto A, Takahashi Y, Ebisu S, Peters MC, In vitro antibacterial effects of the dentin primer of Clearfil Protect Bond. Dent Mater 2006;22: 527-32.
• Settembrini L, Boylan R, Strassler H, Scherer W. A comparison of antimicrobial activity of etchants used for a total etch technique. Oper Dent 1997;22:84-8.
• Karacaer O, Dincer C, Mısırlıgıl A, Aydın C. Çeşitli dentin bonding ajanların antimikrobiyel aktivitelerinin araştırılması. Atatürk Üniv Diş Hek Fak Derg 1995; 5:24-9
• Asmussen E, Munksgaard EC, Bonding of restorative resins to dentine: status of dentine adhe- sives and impact on cavity design and filling tech- niques. Int Dent J 1988;38:97-104.
• Emilson CG, Bergenholtz G. Antibacterial activity of dentinal bonding agents. Quintessence Int 1993;24:511-5.
• Hansen EK, Asmussen E, Comparative study of dentin adhesives, Scand J Dent Res 1985;93:280-7.
• Prati C, Nucci C, Montanari G. Shear bond strength and microleakage of dentin bonding sys- tems. J Prosthet Dent 1991;65:401-7.
• Feuerstein O, Matalon S, Slutzky H, Weiss EI. Antibacterial properties of self-etching dental adhesive systems. J Am Dent Assoc 2007;138:349- 54; 396-8.
• Imazato S, Kinomoto Y, Tarumi H, Torii M, Russell RR, McCabe JF. Incorporation of antibacterial monomer MDPB in dentin primer. J Dent Res 1997;76:768-72.
• http://www.kurarayturkey.com/scripts/ urun.asp?ID=10&page=default.asp&results= (Erişim tarihi: 10.09.2009)
• Imazato S, Ehara A, Torii M, Ebisu S. Antibacterial activity of dentine primer containing MDPB after curing. J Dent 1998;26:267-71.
• Imazato S. Antibacterial properties of resin composites and dentin bonding systems. Dent Mater 2003;19:449–57.
• Korkmaz Y, Ozalp M, Attar N. Comparison of the antibacterial activity of different self-etching primers and adhesives. J Contemp Dent Pract 2008;9:57-64.
• Svanberg M, Mjör IA, Orstavik D. Mutans streptococci in plaque from margins of amalgam, composite, and glass-ionomer restorations. J Dent Res 1990;69:861-4.
• Loesche WJ, Role of Streptococcus mutans in human dental decay. Microbiol Rev 1986;50:353- 80.
• Emilson CG, Ericson T, Lilja J, Heyden G. Effect of chlorhexidine on human oral streptococci. J Periodontal Res 1972;7:189-91.
• Ateşağaoğlu Kaya A, Ömürlü H, Sultan N, Sipahioğlu B. Farklı dentin bağlayıcı sistemlerin antimikrobiyal özelliklerinin in vitro değerlen- dirilmesi. GÜ Dişhek Fak Derg 2008;25:15-22.
• Imazato S, Kuramoto A, Kaneko T, Ebisu S, Russell RRB. Comparison of antibacterial activity of simplified adhesive systems. Am J Dent 2002;5: 356–60.
• Imazato S, Kaneko T, Takahashi Y, Noiri Y, Ebisu S. In vivo Antibacterial Effects of Dentin Primer Incorporating MDPB. Oper Dent 2004; 29:369–75.
• Yıldırım S, Uçan US. Farklı dentin bonding sistemlerin antibakteriyel etkıilerinin karşılaştırılma- sı. GÜ Dişhek Fak Derg 2005;22:1-5.
• Bagis YH, Kosem G, Yamanel K, In vitro evaluation of antibacterial effects of dentin bonding agents on streptococcus mutans, AÜ Diş Hek Fak Derg 2001; 28: 279-85.