Effect the some heavy metals on carbonic anhydrase enzymes activities from non-tumour and tumour human stomach

Yıl 2015, Cilt: 1 Sayı: 1, 25 - 28, 20.05.2015

Emrah Yerlikaya Ramazan Demirdağ Ömer Küfrevioğlu Cemal Gündoğdu

Öz

In this study, in vitro effects of certain heavy metals on the human carbonic anhydrase enzyme were examined. Inhibitory effects of metal ions ( Pb²⁺, Cu²⁺, Fe²⁺,Cr²⁺, Al³⁺, Ni²⁺, Mn²⁺, Cd²⁺, Zn²⁺, and Mg²⁺) were observed in tumour and non-tumour tissue. IC₅₀ values were calculated for metals. The Cu²⁺, Zn²⁺, Ni²⁺, Cd²⁺ and Mg²⁺ IC₅₀ values of tumour tissue were calculated as 0.034mM, 0.426mM, 0.597mM, 0.878mM and 2.52mM respectively. The Cu²⁺, Zn²⁺, Ni²⁺, Cd²⁺ and Mg²⁺  IC₅₀ values of non-tumour tissue were calculated as 0.067mM, 0.991mM, 1.065mM, 1.724mM and 6.13mM respectively. Carbonic anhydrase activity was measured as described by Wilbur and Anderson. Hydratase activity was used to determine IC₅₀ values. In this study, tumour and non-tumour human stomach tissue was selected due to the fact that among the diseases, stomach cancer has one of the highest mortality rates. Stomach cancer, a type of cancer effecting the digestive system, is a fatal disease in living systems.  The effects of metals on the CA enzyme were investigated due to the extremely important role that CA enzymes play in living beings.

Anahtar Kelimeler

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Kaynakça

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