Comparison of Cold-hardiness Levels of Wheat Cultivars Based on Alterations in Alternative Oxidase Protein Level

Year 2019, Volume: 5 Issue: 2, 10 - 15, 15.12.2019

Rahmi Dumlupınar , Nilay Akbulut Hülya Türk Bagher Sheikhi Didani

Abstract

Plant mitochondrial electron transport chain consists of
two terminal oxidases: the cytochrome oxidase (COX) and alternative oxidase
(AOX). AOX pathway is known to be alternative respiration or cyanide-resistant
respiration and linked to stress tolerance of plants. This study aimed to
assess cold-hardening levels of four different cultivars of wheat by comparing
alterations in protein levels of alternative oxidase, which is responsible for
alternative respiration. The AOX protein levels of Yıldırım and Dogu-88
cultivars were markedly higher than those of Ayyıldız and Alpaslan cultivars in
control conditions, which indicate that alternative respirations of Yıldırım
and Dogu-88 cultivars are more active than those of the other cultivars. Cold
stress resulted significant increases AOX protein levels of all cultivars in
comparison to their controls. The increases rates were 47, 34, 31, and 70% in Yıldırım,
Doğu-88, Ayyıldız, and Alpaslan cultivars, respectively. These findings
revealed that although all cultivars tried to resist to cold stress by
improving their alternative respirations, Yıldırım and Dogu-88 cultivars are
more tolerant to cold stress than the other cultivars.

Keywords

Alternative oxidase, cold stress, wheat, immuno-blot analysis

Supporting Institution

Atatürk Üniversitesi

Project Number

2012-1326

Thanks

This work was supported by a grant from the research funds appropriated to Ataturk University, Erzurum, Turkey (2012-1326).

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