egejfas Ege Journal of Fisheries and Aquatic Sciences 2148-3140 Ege Üniversitesi 10.12714/egejfas.40.3.03 Fish Biology Balık Biyolojisi Göynük Çayı'nda (Bingöl, Türkiye) Garra rufa'nın (Heckel, 1843) antioksidan enzim aktivitelerindeki mevsimsel değişimleri Seasonal changes in antioxidant enzyme activities of Garra rufa (Heckel, 1843) in Göynük Stream (Bingöl, Türkiye) https://orcid.org/0000-0003-1888-4388 Kırıcı Muammer BİNGÖL ÜNİVERSİTESİ https://orcid.org/0000-0001-6070-9765 Sen Özdemir Nurgül BINGOL UNIVERSITY https://orcid.org/0000-0002-0363-4848 Caf Fatma BINGOL UNIVERSITY https://orcid.org/0000-0002-9047-0430 Koyun Mustafa BILECIK SEYH EDEBALI UNIVERSITY 09 15 2023 40 3 174 181 03 06 2023 07 10 2023 Copyright © 1984, Su Ürünleri Dergisi 1984 Su Ürünleri Dergisi

Bu çalışmada, Murat Nehri’nin en önemli kollarından biri olan Göynük Çayı’ndan yakalanan Garra rufa (Heckel, 1843) balıklarının solungaç, böbrek, kas ve karaciğer dokularında mevsimlere bağlı olarak antioksidan enzim aktivitelerindeki seviyelerindeki değişimler incelendi. Bu amaç doğrultusunda balıklar, her ay düzenli olarak belirlenen iki istasyondan (Garip ve Ilıcalar) yakalanarak laboratuvara getirildi. Dokularda enzim aktiviteleri ve MDA seviyesi spektrofotometrik yöntemlerle belirlendi. Çalışma sonucunda, genel olarak iki istasyon arasında tüm dokularda parametreler arasındaki fark istatistiki olarak önemli bulunmuştur (p < 0.05). Ancak karaciğer dokusunda GR enziminde tüm mevsimlerde istasyonlar arasındaki fark istatistiki olarak önemli olmadığı belirlendi. Bununla beraber, her iki istasyonda da genel olarak mevsimler arasındaki farklılıkların önemli olduğu gözlemlendi. Çalışmada, GR ve G6PD enzim aktiviteleri diğer enzim aktiviteleri ile kıyaslandığında düşükken, CAT ve SOD aktiviteleri ise diğerlerinden yüksektir. Dokular arasında ise kas dokusundaki enzim aktiviteleri, diğer dokulardaki aktivitelerden daha düşüktür.

In this study, seasonal variations of antioxidant enzyme activities (superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione reductase (GR) and glucose 6-phosphate dehydrogenase (G6PD)) and malondialdehyde (MDA) levels in gill, kidney, muscle and liver tissues of Garra rufa (Heckel, 1843) caught from Göynük Stream, one of the most important branch of Murat River, were investigated. Fish samples were caught from two stations (Garip and Ilıcalar), which are determined regularly every month, and brought to the laboratory. The levels of biomarkers in tissues were determined by spectrophotometric methods. It was determined that the difference between the parameters in the studied tissues was statistically significant (P < 0.05) between the two stations, but the difference between the stations in the liver tissue in all seasons in GR enzyme was not statistically significant. However, it was determined that the differences between the seasons were significant in both stations. GR and G6PD enzyme activities were found to be lower than other enzyme activities among the enzyme groups studied, but CAT and SOD enzyme activities were found to be higher than the other enzymes., enzyme activities in muscle tissue are lower than activities in the other tissues among tissues.

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