Isolation and molecular identification of extreme halophilic archaea from Çamaltı Saltern.

Yıl 2008, Cilt: 25 Sayı: 2, 117 - 121, 01.06.2008

İhsan Yaşa Özge Kahraman Ebru Tekin Ali Koçyiğit

Öz

Anahtar Kelimeler

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Çamaltı Tuzlasından Ekstrem Halofilik Archaea İzolasyonu ve Moleküler Karakterizasyonu.

Öz

Ekstrem halofilik mikroorganizmalar yüksek tuz koşullarında yaşamaya adapte olmuş bu nedenle biyoteknoloji alanında ilgi gören canlılardır. Bu araştırmada İzmir Çamaltı Tuzlası’ndan alınan örneklerden ekstrem halofilik archaea izolasyonu gerçekleştirilerek bu mikroorganizmaların biyokimyasal testlerinin yanı sıra moleküler karakterizasyonları yapılmıştır. 16S rDNA analizleri sonucu 1, 4, 6, 7, 10, 11, 13 nolu izolatlar %99 oranında Haloferax alexandrinus, 5 nolu izolat %98 oranında Haloferax alexandrinus, 12 nolu izolat %96 benzerlik oranıyla Haloferax alexandrinus, 3 nolu izolat %99 Haloferax sp. HSC4, 8 nolu izolat %97 Haloferax YT 228, 2 nolu izolat %99 Halobacterium salinarum R1 strain, 9 nolu izolat %97 Halobacterium salinarum olarak tanılanmıştır

Anahtar Kelimeler

Ekstrem halofilik mikroorganizmalar, Çamaltı tuzlası, archaea, 16S rDNA, moleküler identifikasyon

Kaynakça

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