Molecular Identification, Virulens and Genetic Diversity of Fusarium species on Wheat

Abstract

Surveys were conducted in Konya, Ankara, Eskisehir, Kayseri, Nevsehir, Aksaray, Yozgat, Kirsehir and Kirikkale province in 2011 and 2012 growing seasons and 1256 diseased wheat samples were collected from 2770 wheat fields. As a result of isolation from this samples 668 Fusarium isolates were obtained belonging to 18 different species. In consequence of DNA sequence analysis, obtained isolates were determined Fusarium acuminatum, F. avenaceum, F. brachygibbosum, F. cerealis, F. chlamydosporum, F. culmorum, F. equiseti, F. graminearum, F. incarnatum, F. lacertarum, F. longipes, F. nivale (Microdochium nivale), F. oxysporum, F. proliferatum, F. redolens, F. solani, F. sporotrichioides, F. tricinctum. Pathogenicity tests performed and it was determined that the most virulent group were respectively, 86,72-96%, 84,67-94% ve 80,67-94,67% with diseases severity F. culmorum, F. tricinctum and F. nivale. The most commonly isolated species was F. nivale. Using phylogenetic analyses based on the internal transcribed spacer sequence data, it was examined by genetic variations between species of Fusarium.

Keywords

• Fusarium spp.,Triticum aestivum,molecular,pathogenicity

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