Comparison of glutathione peroxidase-1 in free divers with their counterparts: A model study for sports informatics

Abstract

Free diving is a popular sport because of many features such as sustainability, eco-friendly and challenges to nature. Due to increased interest in recent years, the number of competitions is also increasing gradually. On the other hand, scientific reports on the understanding of longer breath-holding mechanisms and metabolisms are still unclear. To provide contributions on this phenomenon, glutathione peroxidase was selected as a model enzyme because of its critical importance in breath-holding. The enzymes from both human and free diving animals were compared by using bioinformatics tools such as ProtParam, Swiss-Model, Clustal Omega and the results are discussed in the present paper. In conclusion, the specific amino acid sequences can be considered in the selection of elite free divers for international competitions to get the best results. However, it should be noted that special training methods should also be applied to have better breath-holding capacities.

Keywords

• apnea
• free diving
• glutathione peroxidase
• GPX-1

References

1. Annen J, Panda R, Martial C, Piarulli A, Nery G, Sanz LR, Valdivia-Valdivia JM, Ledoux D, Gosseries O. Laureys S. 2021. Mapping the functional brain state of a world champion freediver in static dry apnea. Brain Struc Func. 226: 2675-2688.
2. Bart RM, Lau H. 2022. Shallow Water Blackout. In StatPearls. StatPearls Publishing. Treasure Island (FL).
3. Bi J, Hu B, Zheng J, Wang J, Xiao W, Wang D. 2015. Characterization of the hypoxia-inducible factor 1 alpha gene in the sperm whale, beluga whale, and Yangtze finless porpoise. Mar Biol 162(6):1201-1213
4. Cormack J. R., Gott J., Kondogiannis S. 2017. Profound Hypopnea With Xenon Anesthesia in a Free Diver. A&A Practice, 8(4): 90
5. Feldkamp SD, DeLong RL, Antonelis GA. 1989. Diving patterns of California sea lions, Zalophus californianus. Can J Zool. 67(4):872-883
6. Gales N, Bowers M, Durban JW, Friedlaender AS, Nowacek DP, Pitman RL, Read AJ, Tyson RB. 2013. Advances in non-lethal research on Antarctic minke whales: biotelemetry, photo-identification and biopsy sampling. Paper SC/65a/IA12 presented to the IWC Scientific Committee.
7. Gasteiger E, Hoogland C, Gattiker A, Wilkins MR, Appel RD, Bairoch A. 2005. Protein identification and analysis tools on the ExPASy server. The Proteomics Protocols Handbook 571-607
8. Heide-Jørgensen MP, Richard PR, Rosing-Asvid A. 1998. Dive Patterns of Belugas (Delphinapterus lencas) in Waters near Eastern Devon Island. Arctic. 17-26

9. Ioannidis G, Lazaridis G, Baka S, Mpoukovinas I, Karavasilis V, Lampaki S, Kioumis I, Pitsiou G, Papaiwannou A, Karavergou A, Katsikogiannis N, Sarika E, Tsakiridis K, Korantzis I, Zarogoulidis K, Zarogoulidis P. 2015. Barotrauma and Pneumothorax. J Thorac Dis. 7(1):S38–S43
10. Klatsky LJ, Wells RS, Sweeney JC. 2007. Offshore bottlenose dolphins (Tursiops truncatus): Movement and dive behavior near the Bermuda Pedestal. J Mammal. 88(1): 59-66
11. Kooyman, GL. 1966. Maximum diving capacities of the Weddell seal, Leptonychotes weddelli. Science 151(3717):1553-1554
12. NOAA Fisheries. 2022. Hawaiian Monk Seal. https://www.fisheries.noaa.gov/species/hawaiian-monk-seal. Accessed 1 Nov 2022
13. Rich C., McAteer K., Leytin V., Binder W. 2019. A free diver with hemoptysis and chest pain. Rhode Island Med J 102(1):33-36
14. Scott T., van Waart H., Vrijdag X. C., Mullins D., Mesley P., Mitchell S. J. 2021. Arterial blood gas measurements during deep open-water breath-hold dives. J Appl Physiol. 130(5): 1490-1495
15. Sievers F, Wilm A, Dineen D, Gibson TJ, Karplus K, Li W, Lopez R, McWilliam H, Remmert M, Söding J, Thompson JD. 2011. Fast, scalable generation of high‐quality protein multiple sequence alignments using Clustal Omega. Mol Syst Biol. 7(1):539
16. Studer G, Rempfer C, Waterhouse AM, Gumienny R, Haas J, Schwede T. 2020. QMEANDisCo—distance constraints applied on model quality estimation. Bioinformatics 36(6):1765-1771
17. Uniprot Consortium. 2021. UniProt: the universal protein knowledgebase in 2021. Nucleic Acids Res 49 no. D1 (2021): D480-D489
18. Wang Y, Luo D, Jiang H, Song Y, Wang Z, Shao L, Liu Y. 2023. Effects of physical exercise on biomarkers of oxidative stress in healthy subjects: A meta-analysis of randomized controlled trials. Open Life Sci. 18(1):20220668
19. Waterhouse A, Bertoni M, Bienert S, Studer G, Tauriello G, Gumienny R, Heer FT, de Beer TA, Rempfer C, Bordoli L, Lepore R. 2018. SWISS-MODEL: homology modelling of protein structures and complexes. Nucleic Acids Res. 46(W1):W296-W303
20. Watkins WA, Daher MA, Fristrup KM, Howald TJ, Di Sciara GN. 1993. Sperm whales tagged with transponders and tracked underwater by sonar. Mar Mammal Sci 9(1):55-67
21. Wilkinson A. 2009. The deepest dive: how far down can a free diver go? New Yorker 24:24-30
22. Zapol WM, Liggins GC, Schneider RC, Qvist J, Snider MT, Creasy RK, Hochachka PW. 1979. Regional blood flow during simulated diving in the conscious Weddell seal. J Appl Physiol. 47(5): 968-973
23. Zeppelin T, Pelland N, Sterling J, Brost B, Melin S, Johnson D, Lea MA, Ream R. 2019. Migratory strategies of juvenile northern fur seals (Callorhinus ursinus): bridging the gap between pups and adults. Sci Rep. 9(1):1-16
24. Zhou K, Pilleri G, Li Y. 1979. Observations on the baiji (Lipotes vexillifer) and the finless porpoise (Neophocaena asiaeorientalis) in the Changjiang (Yangtze) River between Nanjing and Taiyangzhou, with remarks on some physiological adaptations of the baiji to its environment. Investig Cetacea. 10:109-20