Multiple shoot regeneration from nodal explants of medicinal aquatic plant Lysimachia nummularia L. under different light emitting diodes (LEDs)

Abstract

Since light emitting diodes (LEDs) have important advantages for plant growth and development, they have been used in tissue culture studies. The light colours of LEDs show different effects on plants. In this study, single and combined effects of white (W), red (R), blue (B) LED lights on multiple shoot regeneration of Lysimachia nummularia L. were investigated. Nodal explants were cultured for eight weeks in a Murashige and Skoog (MS) nutrient medium supplemented with 0.10 mg/L indole butyric acid (IBA) and 0.20-0.80 mg/L Thidiazuron (TDZ). 100% shoot regeneration frequencies in all three hormone applications were recorded under 2W:1R: 1B and 1W: 1R: 1B LED lights. The maximum number of shoots was determined under 1W:2R:1B LED lights as 17.47, 16.16 and 13.46 shoots/explant in the MS nutrient medium added 0.40 mg/L TDZ + 0.10 mg/L IBA, 0.20 mg/L TDZ + 0.10 mg/L and 0.80 mg/L TDZ + 0.10 mg/L IBA, respectively. The minimum number of shoots was recorded under blue LED. The longest shoot was obtained from explants under 1W:1R:2B LED light in all environments. Excessive use of TDZ in the culture medium adversely affected shoot length. Since there was a dense root in replication experiments, no rooting was performed. Plants produced in the culture environment have been successfully adapted to external conditions. As a result, the study showed the effectiveness of the LED lights on the production of L. nummularia and their superiority over the fluorescent lights.

Keywords

• Tissue culture,Light effect,LED,Shoot regeneration

Farklı ışık yayan diyotlar (LED) altında tıbbi sucul bitki Lysimachia nummularia L.'nın boğum eksplantlarından çoklu sürgün rejenerasyonu

Abstract

Işık yayan diyotlar (LED) bitki büyüme ve gelişimi için önemli avantajlara sahip olduklarından dolayı doku kültürü çalışmalarında kullanılmaya başlanmıştır. LED’lerin ışık renkleri bitkiler üzerinde farklı etkiler göstermektedir. Bu çalışmada, beyaz (B), kirmizi (K), mavi (M) LED ışıkların L. nummularia'nın çoklu sürgün rejenerasyonu üzerine tek ve kombine etkileri incelenmiştir. Boğum eksplantları 0,10 mg/L indol butirik asit (IBA) ve 0,20-0,80 mg/L Thidiazuron (TDZ) eklenmiş Murashige ve Skoog (MS) besin ortamında sekiz hafta süre ile kültürlenmiştir. Her üç hormon uygulamasında %100 sürgün rejenerasyon frekansları 2B:1K:1M ve 1B:1K:1M LED ışıkları altında kaydedilmiştir. En fazla sürgün sayısı 1B:2K:1M LED ışıklar altında sırasıyla 17,47, 16,16 ve 13,46 adet olarak sırasıyla 0,40 mg/L TDZ + 0,10 mg/L IBA, 0,20 mg/L TDZ + 0,10 mg/L IBA ve 0,80 mg/L TDZ + 0,10 mg/L IBA eklenmiş MS besin ortamında belirlenmiştir. En az sayıda sürgünler ise mavi LED altında kaydedilmiştir. En uzun sürgünler tüm ortamlarda 1B:1K:2M LED ışık altındaki  eksplantlardan elde edilmiştir. TDZ’nin kültür ortamında fazla kullanılması sürgün uzunluğunu olumsuz etkilemiştir. Çoğaltım denemelerinde yoğun kök oluştuğundan dolayı ayrıca köklendirme yapılmamıştır. Kültür ortamında üretilen bitkiler dış koşullara başarıyla alıştırılmıştır. Sonuç olarak çalışma, LED ışıkların L. nummularia’nın üretimindeki etkinliğini ve flouresan ışıklara göre üstünlüğünü göstermiştir.

Keywords

• Doku kültürü,Işık etkisi,LED,Shoot rejenerasyonu

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