Protective role of apilarnil and acute toxic effect of LPS on kidney DNA

Abstract

Lipopolysaccharide as an endotoxin forms part of the cell wall of gram-negative bacteria and is responsible for initiating an acute inflammation after entering live tissue. In this study, male rats were divided into eight groups as control and treatment groups. Treatment groups are Apilarnil 2, 4 and 8 mg/kg body weight (bw) increasing dose groups, LPS-treated group (LPS, 30 mg/kg bw was given to the LPS group intraperitoneally), LPS+Apilarnil increased dose groups of 2,4 and 8 mg/kg bw. Apilarnil increased doses of 2, 4 and 8 mg/kg were used, and 1 ml oral gavage was administered to the rats. LPS was given to the LPS group (30 mg/kg bw) intraperitoneally. Changes in DNA structure of kidney cells by comet assay were compared with control group after 6 hours. When LPR-treated group and LPS+Apilarnil applied group were compared, DNA damage increased significantly after 6 hours.  In the LPS+Apilarnil treated group, the tail percentage DNA, tail length and tail moment were significantly reduced at the end of the application period. In conclusion, the use of high doses of Apilarnil with different doses against LPS.

Keywords

• LPS,Apilarnil,Toxicity,DNA,Kidney

LPS’nin böbrek DNA’sı üzerine akut toksik etkisi ve apilarnilin koruyucu rolü

Abstract

Bir endotoksin olarak  lipopolisakarit (LPS), gram negatif bakterilerin hücre duvarının bir parçasını oluşturur ve canlı dokuya girdikten sonra akut bir iltihabın başlatılmasından sorumludur. Bu çalışmada erkek sıçanlar kontrol ve uygulama grupları olmak üzere toplam sekiz gruba ayrılmıştır. Uygulama grupları: Apilarnil’in 2, 4 ve 8 mg/kg vücut ağırlığı (va) artan doz grupları, LPS-uygulanan grup (30 mg/kg va intraperitonel yoldan LPS verildi), LPS+ Apilarnil’in 2, 4 ve 8 mg/kg va artan doz grupları.  Apilarnil’in 2, 4 ve 8 mg/kg artan dozları sıçanlara 1 ml oral gavaj olarak verildi. Komet yöntemi ile böbrek hücrelerinin DNA yapısındaki değişiklikler 6 saat sonunda kontrol grubuyla karşılaştırıldı. LPS-uygulanan grupla LPS+Apilarnil uygulanan grup karşılaştırıldığında, DNA hasarı 6 saatin sonunda önemli ölçüde artmıştır. LPS+Apilarnil uygulanan grupta, uygulama süresi sonunda kuyruk yüzde DNA'sı, kuyruk uzunluğu ve kuyruk momenti önemli ölçüde azalmıştır. Sonuç olarak, LPS'ye karşı farklı dozlarda Apilarnil’in kullanılan gruplar karşılaştırıldığında yüksek dozda Apilarnil’in kullanılmasının daha koruyucu olduğu tespit edilmiştir.

Keywords

• LPS,Apilarnil,Toksisite,DNA,Böbrek

References

1. Acemi A, Bayrak B, Çakır M, Demiryürek E, Gün E, El Gueddari NE, Özen F 2018. Comparative analysis of the effects of chitosan and common plant growth regulators on in vitro propagation of Ipomoea purpurea (L.) Roth from nodal explants. In Vitro Cell Dev Biol Plant 54(5): 537-544.
2. Bourget CM 2008. An introduction to light-emitting diodes. HortScience 43(7): 1944-1946.
3. Dănilă E, Lucache DD 2013. Cost effectiveness of growing plant lighting system. Journal of Electrical Engineering 13(4): 224-229.
4. Debnath SC 2005. Micropropagation of lingonberry: influence of genotype, explant orientation, and overcoming TDZ-induced inhibition of shoot elongation using zeatin. HortScience 40(1): 185-188.
5. Dogan M 2018a. In vitro shoot regeneration of Limnophila aromatica (Lamk.) Merr. from nodal and internodal explants. Iğdır Univ J Inst Sci & Tech 8: 77-84.
6. Dogan M 2019a. Multiple shoot regeneration via indirect organogenesis from shoot tip and nodal meristem explants of Ceratophyllum demersum L. J Anim Plant Sci 29: 568-577.
7. Dogan M 2019b. Callus formation from full leaf and leaf parts of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Acta Biologica Turcica 32: 78-83.
8. Dogan M 2019c. In vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze. Ant J Bot 3: 1-6.

9. Dogan M 2018b. In vitro micropropagation from nodal explants of the medicinal plant Lysimachia nummularia L. KSU J Agric Nat 21(6): 875-881.
10. Dogan M, Emsen B 2018. Anti-cytotoxic-genotoxic influences of in vitro propagated Bacopa monnieri L. Pennell in cultured human lymphocytes. Eurasian J Bio Chem Sci 1(2): 48-53.
11. Dougher T, Bugbee B 2001. Differences in the response of wheat, soybean and lettuce to reduced blue radiation. Photochem Photobiol 73: 199- 207.
12. Emsen B, Dogan M 2018. Evaluation of antioxidant activity of in vitro propagated medicinal Ceratophyllum demersum L. extracts. Acta Sci Pol-Hortoru 17: 23-33.
13. Fatonah S, Isda MN 2018. In vitro shoot regeneration of tembesu (Fagraea fragrans Roxb.) from seed explants on different concentrations of sucrose and honey. Biosci Res 15(2): 655-662.
14. Gupta SD, Sahoo TK 2015. Light emitting diode (LED)- induced alteration of oxidative events during in vitro shoot organogenesis of Curculigo orchioides Gaertn. Acta Physiol Plant 37(11): 233.
15. Heo J, Lee C, Chakrabarty D, Paek K 2002. Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regulat 38: 225-230.
16. Ledbetter DI, Preece JE 200). Thidiazuron stimulates adventitious shoot production from Hydrangea quercifolia Bartr. leaf explants. Sci Hort 101(1-2): 121- 126.
17. Lin KH, Huang MY, Huang WD, Hsu MH, Yang ZW, Yang CM. 2013. The effects of red, blue, and white light- emitting diodes on the growth, development, and edible quality of hydroponically grown lettuce (Lactuca sativa L. var. capitata), Sci Hort 150: 86-91.
18. Lu YX, Godo T, Fujiwara K, Guan KY, Mii M 2013. Effects of nitrogen source and wavelength of led-light on organogenesis from leaf and shoot tip cultures in Lysionotus pauciflorus maxim. Propag Ornam Plants 13: 174-180.
19. Morrow RC 2008. LED lighting in horticulture. HortScience 43(7): 1947-1950.