Effect of zinc oxide nanoparticle on acetylcholinesterase enzyme activity in Galleria mellonella L. (Lepidoptera: Pyralidae) larvae

Abstract

Zinc oxide nanoparticles (ZnO NP) is widely used in many field, food and packaging additives industry, cosmetics, sunscreens etc., with the devolopment of nanotechnology. Nanoparticles can readily pass through biological membranes and so cause negative effects and accumulation at the organ, tissue, cell and molecular level. In this study, 30 mg/l and 30 µg/mL concentrations of ZnO NP were injected into the first proleg of the last instar Galleria mellonella larvae. Then, the antioxidant stress level in fat body isolated from the control and application groups was determined by determining the AChE enzyme activity. As a result of the data obtained, AChE activity in fat body of G. mellonella larvae exposed to ZnO NP showed statistically significant increases and decreases in both groups compared to control. It have shown that, understanding the biochemical effects and determine to the possible changes of macromolecules in fat body of G.mellonella larvae, which is a simple, inexpensive and fast to produce model for toxicity studies, will help develop methods that have less adverse effects on the environment and lead to immunological and physiological studies.

Keywords

• Galleria mellonella
• Acetylcholinesterase
• Zinc oxide
• Nanoparticle

Çinko Oksit Nanopartikülünün Galleria mellonella (Lepidopetra:Pyralidae) (L.) Larvalarında Asetilkolinesteraz Enzim Aktivitesi Üzerine Etkisi

Abstract

Çinko oksit nanopartikülü (ZnO NP), nanoteknolojinin gelişmesiyle birçok alanda (gıda ve ambalaj katkı maddeleri sanayii, kozmetik, güneş kremleri v.b.) yaygın olarak kullanılmaktadır. Biyolojik membranlardan rahatlıkla geçebilen nanopartiküller organ, doku, hücre ve moleküler düzeyde olumsuz etkilere ve birikime neden olabilmektedirler. Yapılan çalışmada, Galleria mellonella son evre larvalarına arka bacaklarından ZnO NP enjeksiyon yoluyla 30 mg/l ve 30 µg/mL derişimlerinde uygulanmıştır. Uygulama sonunda kontrol ve deneme gruplarından izole edilen yağ dokudaki oksidatif stres düzeyi AChE enzim aktivitesinin tespiti ile belirlenmiştir. Elde edilen veriler sonucunda, ZnO NP maruz kalan G. mellonella larvalarının yağ dokudaki AChE aktivitesinde kontrole göre her iki uygulama grubunda da istatistiki olarak belirgin artış ve azalışlar göstermiştir. Toksisite çalışmaları için basit, ucuz ve hızlı üretilebilen bir model olan G. mellonella larvalarının yağ dokusundaki makromoleküllerin olası değişikliklerinin tespit edilmesi ile biyokimyasal etkilerin anlaşılması, çevreye daha az olumsuz etkisi olan yöntemlerin geliştirilmesine yardımcı olacağı ve elde edilen veriler doğrultusunda yapılacak immünolojik ve fizyolojik çalışmalara yol gösterebileceği düşünülmektedir

Keywords

• Galleria mellonella
• Asetilkolinesteraz
• Çinko oksit

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