The Synthesis, Characterization and Determination of Antioxidant Activity of Rutin Loaded Chitosan Nanoparticles

Yıl 2020, Cilt: 3 Sayı: 2, 93 - 99, 29.12.2020

Gizem Beyoğlu , Özlem Araç , Derya Taşkın , Pelin Pelit Arayıcı , Kadriye Kızılbey , Serap Derman

Öz

Rutin, one of the quercetin glycosides, is a flavanoid mainly found in different parts of plants such as fruit peels, leaves, flowers and roots. Although it has antioxidant, antimicrobial and chelating properties due to its chemical structure and its bioavailability in living system is very low due to its poor solubility. In this study, to increase the bioavailability of the rutin molecule, rutin loaded chitosan nanoparticles were synthesized by ionic gelling method and their in vitro releasing studies and antioxidant activities were investigated. The encapsulation efficiency and effective loading capacity of the rutin loaded chitosan nanoparticles were analyzed by UV-Vis spectrophotometer, and particle size, multiple distribution index and zeta potential values were analyzed by Photon Correlation Spectroscopy. Polymer-active molecule interactions of these nanoparticles were examined by FT-IR spectroscopy. The optimum nanoparticle for releasing studies was determined as Rutin-loaded Chitosan Nanoparticles3 (R-Chi NPs3) considering the encapsulation efficiency and effective loading capacity. In-vitro releasing study was continued for 17 days and at the end of this time 88.8 % rutin release was observed. The antioxidant activity study of the synthesized nanoparticles was carried out by DPPH method, and the antioxidant activity of R-Chi NPs3 nanoparticles increased due to increasing concentration and it was determined that they showed 88.1% antioxidant activity with 0.8 mg/mL R-Chi NPs3 concentration. This study is a research study showing that advanced biotechnological systems that can make controlled release can be designed to increase the effects of molecules with low bioavailability.

Anahtar Kelimeler

Rutin, Chitosan, Nanoparticle, Antioxidant Activity

Rutin Yüklü Kitosan Nanopartiküllerinin Sentezi, Karakterizasyonu ve Antioksidan Aktivitesinin Değerlendirilmesi

Öz

Kuersetin glikozitlerinden biri olan rutin esas olarak meyve kabukları, yaprakları, çiçekleri ve kökleri gibi bitkilerin farklı kısımlarında bulunan bir flavanoiddir. Kimyasal yapısı nedeniyle antioksidan, antimikrobiyal ve şelatlayıcı özellikleri bulunan rutin molekülünün zayıf çözünürlüğü nedeniyle canlı sistemde biyoyararlanımı oldukça düşüktür. Bu çalışmada, rutin molekülünün biyoyararlanımını arttırmak için iyonik jelleşme yöntemi kullanılarak rutin yüklü kitosan nanopartikülleri sentezlenmiş ve in vitro ortamda salımları ve antioksidan aktiviteleri araştırılmıştır. Üretilen rutin yüklü kitosan nanopartiküllerin enkapsülasyon etkinliği ve etken yükleme kapasitesi UV-Vis spektrofotometre kullanılarak; parçacık boyutu, çoklu dağılım indeksi ve zeta potansiyel değerleri ise Foton Korelasyon Spektroskopisi ile analiz edilmiştir. Bu nanopartiküllerdeki polimer-etken molekül etkileşimleri FT-IR ile incelenmiştir. Enkapsülasyon etkinliği ve etken yükleme kapasitesi göz önüne alınarak salım deneyleri için optimum nanopartikül Rutin yüklü Kitosan Nanopartikül3 (R-Chi NPs3) olarak belirlenmiştir. In vitro salım çalışması 17 gün devam ettirilmiş ve bu sürenin sonunda %88.8 oranında rutin salımı gözlenmiştir. Sentezlenen nanopartiküllerin antioksidan aktivite çalışması ise DPPH yöntemi ile gerçekleştirilmiş olup R-Chi NPs3 nanopartiküllerin antioksidan aktivitesinin artan konsantrasyona bağlı olarak arttığı ve 0.8 mg/mL R-Chi NPs3 içeren örneğin %88.1 antioksidan aktivite gösterdiği belirlenmiştir. Bu çalışma, biyoyararlanımı düşük olan moleküllerin etkilerini arttırmak için kontrollü salım yapabilecek gelişmiş biyoteknolojik sistemlerin tasarlanabileceğini gösteren bir araştırma niteliğindedir.

Anahtar Kelimeler

Rutin, Kitosan, Nanopartikül, Antioksidan Aktivite

Kaynakça

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