Effect of vitamin B3 supplementation on glutathione redox cycle

Yıl 2022, Cilt: 5 Sayı: 1, 1 - 4, 08.06.2022

Adem Keskin

https://doi.org/10.46239/ejbcs.1003863

Cited By: 1

Öz

In this study, the effect of vitamin B3 supplement given to rats was investigated on glutathione redox cycle by looking at glutathione peroxidase and glutathione s transferase activities. 20 Wistar albino male rats were used. Vitamin B3 supplement was given to one of the two groups that were formed. The other group was determined as the control group. 360 mg/kg/day vitamin B3 supplement was given by oral gavage method for 10 days. At the end of 10 days, intracardiac blood samples were taken. Glutathione peroxidase activity level was determined as 1033.44±198.05 U/L in the vitamin B3 supplement group and 526.00±99.54 U/L in the control group. The glutathione peroxidase activity level of the vitamin B3 supplemented group was found to be statistically significantly higher than the glutathione peroxidase activity level of the control group (p<0.001, t=7.239). This difference has huge impact size (d=3.24). Glutathione s transferase activity level was determined as 7.50±0.67 U/mL in the vitamin B3 supplement group and 6.50±0.97 U/mL in the control group. The glutathione s transferase activity level of the group given vitamin B3 supplementation was found to be statistically significantly lower than the glutathione s transferase activity level of the control group (p=0.015, t=2.698). This difference has a very large effect size (d=1.20). As a result, vitamin B3 supplementation led to an increase in glutathione peroxidase activity at a huge effect size and a decrease in glutathione s transferase activity at a very large effect size.

Anahtar Kelimeler

Vitamin B3, Glutathione peroxidase, Glutathione s transferase, Glutathione redox cycle

Kaynakça

• Ashoori M, Saedisomeolia A. 2014. Riboflavin (vitamin B2) and oxidative stress: a review. Br J Nutr. 111(11):1985-1991.
• Bachhawat AK, Yadav S. 2018. The glutathione cycle: Glutathione metabolism beyond the γ-glutamyl cycle. IUBMB Life. 70(7):585-592.
• Belorgey D, Antoine Lanfranchi D, Davioud-Charvet E. 2013. 1,4-Naphthoquinones and Other NADPH-Dependent Glutathione Reductase- Catalyzed Redox Cyclers as Antimalarial Agents. Curr Pharm Des. 19(14):2512-2528.
• Brigelius-Flohe R, Flohe L. 2020. Regulatory Phenomena in the Glutathione Peroxidase Superfamily. Antioxid Redox Signal. 33(7):498-516.
• Cohen J. 1988. Statistical Power Analysis for the Behavioral Sciences. 2nd edn. Lawrence Erlbaum Associates. New York.
• Couto N, Wood J, Barber J. 2016. The role of glutathione reductase and related enzymes on cellular redox homoeostasis network. Free Radic Biol Med. 95:27-42.
• Garcion E, Wion-Barbot N, Montero-Menei C, Berger F, Wion D. 2002. New clues about vitamin D in the nervous system. Trends Endocrinol Metab. 13:100-105.
• Hayes JD, Flanagan JU, Jowsey IR. 2005. Glutathione Transferases. Annu Rev Pharmacol Toxicol. 45:51-88.
• Huang, J, Tian, L, Wu, X, Yang H, Liu Y. 2010. Effects of dietary riboflavin levels on antioxidant defense of the juvenile grouper Epinephelus coioides . Fish Physiol Biochem. 36:55-62.
• Jones CI, Zhu H, Martin SF, Han Z, Li Y, Alevriadou BR. 2007. Regulation of antioxidants and phase 2 enzymes by shear-induced reactive oxygen species in endothelial cells. Ann Biomed Eng. 35:683-693.
• Kwon WY, Suh GJ, Kim KS, Jung YS, Kim SH, Lee R, et al. 2018. Niacin And Selenium Attenuates Brain Injury After Cardiac Arrest By Upregulatıng Dj-1-Akt Signaling. Crit Care Med. 46(1):125.
• Lappas M, Permezel M. 2011. The anti-inflammatory and antioxidative effects of nicotinamide, a vitamin B3 derivative, are elicited by FoxO3 in human gestational tissues: implications for preterm birth. J Nutr Biochem. 22(12):1195-1201.
• Liang H, Liu Q, Xu, J. 1999. The effect of riboflavin on lipid peroxidation in rats. Wei Sheng Yan Jiu. 28:370–371.
• Makarov MV, Trammell SAJ, Migaud ME. 2019. The chemistry of the vitamin B3 metabolome. Biochem Soc Trans. 47(1):131–147.
• Mahmoud YI, Mahmoud AA. 2016. Role of nicotinamide (vitamin B3) in acetaminophen-induced changes in rat liver: Nicotinamide effect in acetaminophen-damged liver. Exp Toxicol Pathol. 68(6):345-354.
• Meyer-Ficca M, Kirkland JB. 2016. Niacin. Adv Nutr. 7(3):556-558
• Noctor G, Queval G, Mhamdi A, Chaouch S, Foyer CH. 2011. Glutathione. TAB. 9:e0142.
• Noctor G, Mhamdi A, Chaouch S, Han Y, Neukermans J, Marquez-Garcia B, Queval G, Foyer CH. 2012. Glutathione in plants: an integrated overview. Plant Cell Environ. 35(2):454-484.
• Oakley A. 2011. Glutathione transferases: a structural perspective. Drug Metab Rev. 43(2):138-151.
• Paglia DE, Valentine WN. 1967. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med. 70(1):158-169.
• Ponce IT, Rezza IG, Delgado SM, Navigatore LS, Bonomi MR, Golini RL, Gimenez MS, Anzulovich AC. 2011. Daily oscillation of glutathione redox cycle is dampened in the nutritional vitamin A deficiency. Biol Rhythm Res. 43(4):351-372.
• Sawilowsky S. 2009. "New effect size rules of thumb". J Mod Appl Stat Methods. 8(2): 467-474.
• Shenhu W, Jie M, Qixuan C, Yuechu X, Yuming C, Huilian Z. 1999. Effect of beta-carotene and riboflavin on lipid peroxidation in rats. Acta Nutr Sin. 21:22-27.
• Tumkiratiwong, P, Tungtrongchitr, R, Migasena, P, Pongpaew P, Rojekittikhun W, Vudhivai N. 2003. Antioxidant enzyme levels in the erythrocytes of riboflavin-deficient and Trichinella spiralis-infected rats. Southeast Asian J Trop Med Public Health. 34:480-485.
• Van Haaften RIM, Haenen GRMM, Evelo CTA, Bast A. 2003. Effect of Vitamin E on Glutathione-Dependent Enzymes. Drug Metab Rev. 35(2-3):215-253.
• Vivancos PD, Wolff T, Markovic J, Pallardo FV, Foyer CH. 2010. A nuclear glutathione cycle within the cell cycle. Biochem. 431(2):169-178.
• Waly M, Al-Attabi Z, Guizani N. 2015. Low Nourishment of Vitamin C Induces Glutathione Depletion and Oxidative Stress in Healthy Young Adults. Prev Nutr Food Sci. 20(3):198-203.
• Williams PA, Harder JM, Foxworth NE, Cochran KE, Philip VM, Porciatti V, et al. 2017. Vitamin B3 modulates mitochondrial vulnerability and prevents glaucoma in aged mice. Science. 355(6326):756-760.
• Xu P, Sauve AA. 2010. Vitamin B3, the nicotinamide adenine dinucleotides and aging. Mech Ageing Dev. 131(4):287-298.