Salicylic acid improves somatic embryogenesis system in triticale using mature embryos
Abstract
The main aim of this study was to define salicylic impacts on the somatic embryo formation ratio. Mature embryos, obtained from seeds, were grown on Murashige and Skoog (MS) medium containing with 1 and 2 mM salicylic acid + 1 mg mL-1 2,4-D. Salicylic acid promoted the somatic embryo formation ratio in both concentrations. Green spots were seen on the callus tissues and developed easily in a salicylic acid medium. Some physiological changes were assayed in both somatic embryos and callus tissues. The results demonstrated that H2O2 and MDA were promoted in somatic embryo tissues. In outcome, it was observed in this study that in vitro salicylic acid might be used to increase somatic embryo number in callus samples of triticale.
Keywords
References
- 1. Catinot J, Buchala A, Abou-Mansour E, Metraux JP. Salicylic acid production in response to biotic and abiotic stress depends on isochorismate in Nicotiana benthamiana. Molecular mechanisms of interorgan crosstalk in health and disease 2008;171.
- 2. Hutchinson MJ, Saxena PK. Acetylsalicylic acid enhances and synchronizes thidiazuron-induced somatic embryogenesis in geranium (Pelargonium x hortorum Bailey) tissues cultures. Plant Cell Reports 1996;15:512-515.
- 3. Luo Y, Wan S, Hui D, Wallace LL. Acclimatization of soil respiration to warming in a tall grass prairie. Nature 2001;413:622-625.
- 4. Hao Z, Hao F, Singh VP, Zhang X. Changes in the severity of compound drought and hot extremes over global land areas. Environmental Research Letters 2018;13-124022.
- 5. Malabadi RB, Teixeira da Silva JA, Nataraja K, Mulgund GS. Shoot tip transverse thin cell layers and 2,4-epibrassinolide in the micropropagation of Cymbidium bicolor Lindl. Floriculture and Ornamental Biotechnology. 2008;2:44–48.
- 6. Mulgund GS, Meti NT, Malabadi RB, Nataraja K, Vijayakumar S. Smoke promoted in vitro seed germination of Pholidota pallida Lindl. Research in Plant Biology 2012;2(2):24-29.
- 7. Ahmadi A, Joudi M, Jonmaohammadi M. Late defoliation and wheat yield: little evidence of post-anthesis source limitation Field Crops Research 2009;113:90–93.
- 8. Ikeuchi M, Sugimoto K, Iwase A. Plant callus: mechanisms of induction and repression. Plant Cell 2013;25(9):3159–73.
- 9. Feher A, Bernula D, Katalib G. The many ways of somatic embryo initiation in Somatic Embryogenesis fundamental aspects and applications, eds V.M. Loyola-Vargas and N. Ochoa-Alejo. Springer International 2016;23-37.
- 10. Yazıcılar B, Böke F, Alaylı A, Nadaroglu H, Gedikli S, Bezirganoglu I. In vitro efects of CaO nanoparticles on Triticale callus exposed to short and long term salt stress. Plant Cell Reports 2021;40:29-42.