The Effects of Various Dental Restorative Materials on Neuroblastoma Cells

Pınar Bayram; Mustafa Duzyol; Esra Düzyol; Selina Aksak Karameşe

EN

The Effects of Various Dental Restorative Materials on Neuroblastoma Cells

Abstract

Introduction: Our aim was to investigate the effects of restorative materials such as composite, compomer and glass ionomer, which are frequently used in dentistry, on SH-SY5Y neuroblastoma cells by evaluating oxidative stress parameters, pro- and anti-inflammatory cytokines and apoptosis markers. Materials and Method: Equa Forte, Dyract AP, Tokuyama Estelite P Quick, Omnichroma, Filtek Z250, SureFil SDR flow restorative materials were used in our study. SH-SY5Y neuroblastoma cells were cultured with restorative materials. Immunohistochemical staining was performed on the experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT levels. One-way ANOVA analysis was used in the statistical evaluation of the obtained results (p<0.05). Results: In the light of the obtained data, it was observed that the dental filling materials were effective in increasing TGF-beta, IL-10, LPO and CAT levels, and decreasing TNF-alpha, IL-1-beta and IL-6 levels. Histological micrographs also supported the issues. When the H-score levels in the Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups. Conclusion: Our study shows that biocompatibility cannot be explained by looking at a single cause. Biocompatibility varies with material content, residual monomer amount and solubility. Although all experimental groups have cytotoxic effects, the least effect is seen in the glass ionomer group.

Keywords

Dental filling materials , SHSY5Y , neuroblastoma , inflammation , apoptosis

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Pınar Bayram
0000-0001-9924-7051
Türkiye

Mustafa Duzyol
0000-0002-8438-1423
Türkiye

Esra Düzyol
0000-0002-5674-6990
Türkiye

Selina Aksak Karameşe ^*
0000-0002-4820-2096
Türkiye

Early Pub Date

October 8, 2023

Publication Date

October 25, 2023

Submission Date

August 31, 2023

Acceptance Date

October 6, 2023

Published in Issue

Year 2023 Volume: 2 Number: 2

IZ

https://izlik.org/JA83PU29KB

APA

Bayram, P., Duzyol, M., Düzyol, E., & Aksak Karameşe, S. (2023). The Effects of Various Dental Restorative Materials on Neuroblastoma Cells. Eurasian Journal of Molecular and Biochemical Sciences, 2(2), 14-20. https://izlik.org/JA83PU29KB

The Effects of Various Dental Restorative Materials on Neuroblastoma Cells

The Effects of Various Dental Restorative Materials on Neuroblastoma Cells

Abstract

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Details

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Subjects

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Early Pub Date

Publication Date

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Acceptance Date

Published in Issue

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