The Effects of Various Dental Restorative Materials on Neuroblastoma Cells

Year 2023, Volume: 2 Issue: 2, 14 - 20, 25.10.2023

Pınar Bayram , Mustafa Duzyol , Esra Düzyol , Selina Aksak Karameşe

Abstract

Introduction: Our aim was to investigate the effects of restorative materials such as composite, compomer and glass ionomer, which are frequently used in dentistry, on SH-SY5Y neuroblastoma cells by evaluating oxidative stress parameters, pro- and anti-inflammatory cytokines and apoptosis markers.
Materials and Method: Equa Forte, Dyract AP, Tokuyama Estelite P Quick, Omnichroma, Filtek Z250, SureFil SDR flow restorative materials were used in our study. SH-SY5Y neuroblastoma cells were cultured with restorative materials. Immunohistochemical staining was performed on the experimental groups with Anti-Bax and Anti-Caspase 9 antibodies. Then, ELISA technique was used to detect TNF-alpha, TGF-beta, IL-1-beta, IL-6, IL-10, LPO and CAT levels. One-way ANOVA analysis was used in the statistical evaluation of the obtained results (p<0.05).
Results: In the light of the obtained data, it was observed that the dental filling materials were effective in increasing TGF-beta, IL-10, LPO and CAT levels, and decreasing TNF-alpha, IL-1-beta and IL-6 levels. Histological micrographs also supported the issues. When the H-score levels in the Caspase 9 labeled micrographs were evaluated, the mean of the control group was lower than the mean of the experimental groups.
Conclusion: Our study shows that biocompatibility cannot be explained by looking at a single cause. Biocompatibility varies with material content, residual monomer amount and solubility. Although all experimental groups have cytotoxic effects, the least effect is seen in the glass ionomer group.

Keywords

Dental filling materials , SHSY5Y , neuroblastoma , inflammation , apoptosis

References

• 1. Rueggeberg FA. From vulcanite to vinyl, a history of resins in restorative dentistry. J Prosthet Dent. 2002, 87, 364–379.
• 2. Modena KC, Casas-Apayco LC, Atta MT, Costa CA, Hebling J, Sipert CR, Navarro MF, Santos CF. Cytotoxicity and biocompatibility of direct and indirect pulp capping materials. J Appl Oral Sci. 2009, 17(6), 544-54.
• 3. Schmalz G, Arenholt-Bindslev D. Biocompatibility of dental materials (Vol. 1). Berlin: 2009, Springer.
• 4. Schmalz G, Groppl F, Hiller KA, Wales KM. Three- dimensional human cell cultures for cytotoxicity testing of dental filling materials. Acta stomatologica Croatica. 2014, 48(2), 99.
• 5. Pelka M, Danzl C, Distler W, Petschelt A. A new screening test for toxicity testing of dental materials. J Dent, 2000, 28(5), 341-345.
• 6. Schweikl H, Hiller KA, Bolay C, Kreissl M, Kreismann W, Nusser A, Steinhauser S, Weiczork J, Vasold R, Schmalz G. Cytotoxic and mutagenic effects of dental composite materials. Biomaterials. 2005, 26(14), 1713-1719.
• 7. Ortengren U, Wellendorf H, Karlsson S, Ruyter IE. Water sorption and solubility of dental composites and identification of monomers released in an aqueous environment. J Oral Rehab, 2001, 28(12), 1106-1115.
• 8. Padbury A, Eber R, Wang HL. Interactions between the gingiva and the margin of restorations. J Clin Periodontol, 2003, 30, 379-385.
• 9. Pettini F, Savino M, Corsalini M, Cantore S, Ballini A. Cytogenetic genotoxic investigation in peripheral blood lymphocytes of subjects with dental composite restorative filling materials. J Biol regular Homeost agents. 2015, 29(1), 229-33.
• 10. Sakaguchi RL, Douglas WH, Peters MC. Curing light performance and polymerization of composite restorative materials. J Dent, 1992, 20, 183-188.
• 11. Rueggeberg FA, Caughman WF, Curtis JW, Davis HC. A predictive model for the polymerization of photo-activated resin composites. Int J Prosthodont, 1994, 7, 159-166.
• 12. Rueggeberg FA, Caughman WF, Curtis JW. Effect of light intensity and exposure duration on cure of resin composite. Oper Dent, 1994, 19, 26-32.
• 13. Flury S, Peutzfeldt A, Lussi A. Influence of increment thickness on microhardness and dentin bond strength of bulk fill resin composites. Dent Mater, 2014, 30, 1104-1112.
• 14. Price RB, Derand T, Loney RW, Andreou P. Effect of light source and specimen thickness on the surface hardness of resin composite. Am J Dent, 2002, 15, 47-53.
• 15. Anusavice KJ, Phillips RW, Shen C, Rawls HR. Phillips' Science of Dental Materials. 2013. Elsevier/Saunders.
• 16. Ceballos L, Fuentes MV, Tafalla H, Martinez, A Flores J, Rodriguez J. Curing effectiveness of resin composites at different exposure times using LED and halogen units. Med Oral Patol Oral Cir Bucal, 2009, 14, 51-56.
• 17. Park J, Chang J, Ferracane J, Lee IB. How should composite be layered to reduce shrinkage stress: incremental or bulk filling? Acad Dent Mater, 2008, 24, 1501-1505.
• 18. Van Ende A, De Munck J, Van Landuyt KL, Poitevin A, Peumans M, Van Meerbeek B. Bulk-filling of high C-factor posterior cavities: effect on adhesion to cavity-bottom dentin. Dent Mater, 2013, 29, 269-277.
• 19. Ferracane JL. Resin composite–state of the art. Dent Mater, 2011, 27, 29-38.
• 20. Bayne SC, Thompson JY, Swift EJ, Stamatiades P, Wilkerson M. A characterization of first-generation flowable composites. J Am Dent Assoc, 1998, 129, 567-577.
• 21. Emami N, Sjodahl M, Soderholm KJ. How filler properties, filler fraction, sample thickness and light source affect light attenuation in particulate filled resin composites. Acad Dental Mater, 2005, 21, 721-730.
• 22. Czasch P, Ilie N. In vitro comparison of mechanical properties and degree of cure of bulk fill composites. Clin Oral Invest, 2013, 17, 227-235.
• 23. Scougall-Vilchis RJ, Hotta Y, Hotta M, Idono T, Yamamoto K. Examination of composite resins with electron microscopy, microhardness tester and energy dispersive X-ray microanalyzer. Dent Mater J, 2009, 28, 102-112.
• 24. Huang TH, Yang CC, Ding SJ, Yeng M, Kao CT, Chou MY. Inflammatory cytokines reaction elicited by root-end filling materials. J Biomed Mater Res B Appl Biomater. 2005, 73(1), 123-8.
• 25. Baek SH, Plenk H Jr, Kim S. Periapical tissue responses and cementum regeneration with amalgam, SuperEBA, and MTA as root-end filling materials. J Endod. 2005, 31(6), 444-9.
• 26. Can İ, Duzyol M, Albayrak M, Aksak Karamese S, Seven N, Dumlu Atalay F, Duzyol E, Gundogdu C. The Harmful Effects of Restorative Dental Filling Materials on The Kidney and Liver Tissues. Ataturk Uni J Vet Med, 2020, 15(3), 207-215.
• 27. Aksak Karamese S, Can I, Duzyol M, Seven N, Albayrak M, Dumlu Atalay F, Duzyol E, Gundogdu C. The Examination of the Reelationship Between Brain Damage and Dental Restorative Filling Materials: An In-vivo Study. Journal of Inonu University Health Services Vocational School, 2020, 8(3), 664-675.