Protective Effects of Acacetin on Testicular Ischemia Reperfusion Injury
Abstract
Objective: Testicular torsion is a medical problem primarily seen in newborns and adolescents and requires urgent treatment.
It is anticipated that the use of antioxidants may be an effective treatment option. Acacetin (ACA) is a natural flavonoid of plant
origin and has attracted research attention in recent years with its powerful antioxidant properties. This study aims to
investigate the therapeutic effects of Acacetin in testicular ischemia-reperfusion (IR) injury. Materials and Methods: Twenty-
eight Wistar rats (male, 12 weeks, 250-300g) were used in the current study. After 1 hour of ischemia, 4 hours of reperfusion
was performed. Acacetin was administered intraperitoneally at a single dose of 25mg/kg. Testicular tissues were analyzed using
genetic, biochemical, and histological methods. Results: IR caused damage to testicular tissues by significantly increasing the
levels of NF-κB (p<0.05), Caspase-3 (p<0.01), TOS (p<0.001), OSI (p<0.001) and decreasing the levels of Bcl-2 and increasing
the levels of TAS, although not significantly. ACA exhibited protective properties against IR damage in testicular tissues by
reversing the effects of IR. When ACA was applied together with IR, Caspase-3 (p<0.01), TOS (p<0.001), and OSI (p<0.001)
levels decreased significantly compared to the IR group. While NF-κB levels decreased insignificantly, TAS and Bcl-2 levels
increased insignificantly. Conclusions: ACA provides protective properties against testicular IR injury.
Keywords
Acacetin, apoptosis, ischemia reperfusion, oxidative stress, testis
Supporting Institution
Tubitak
Project Number
1919B012105612
Thanks
The authors thank Dr. Nurhan
Akaras for her support in the histological examination.
This study was supported by Tubitak 2209 project
(2209/A, Project: 1919B012105612).
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