Protective Effects of Acacetin on Testicular Ischemia Reperfusion Injury

Year 2024, , 22 - 30, 25.02.2025

Yusuf Kenger , Hasan Şimşek

https://doi.org/10.5281/zenodo.14932929

Abstract

Objective: Testicular torsion is a medical problem primarily seen in newborns and adolescents and requires urgent treatment.
It is anticipated that the use of antioxidants may be an effective treatment option. Acacetin (ACA) is a natural flavonoid of plant
origin and has attracted research attention in recent years with its powerful antioxidant properties. This study aims to
investigate the therapeutic effects of Acacetin in testicular ischemia-reperfusion (IR) injury. Materials and Methods: Twenty-
eight Wistar rats (male, 12 weeks, 250-300g) were used in the current study. After 1 hour of ischemia, 4 hours of reperfusion
was performed. Acacetin was administered intraperitoneally at a single dose of 25mg/kg. Testicular tissues were analyzed using
genetic, biochemical, and histological methods. Results: IR caused damage to testicular tissues by significantly increasing the
levels of NF-κB (p<0.05), Caspase-3 (p<0.01), TOS (p<0.001), OSI (p<0.001) and decreasing the levels of Bcl-2 and increasing
the levels of TAS, although not significantly. ACA exhibited protective properties against IR damage in testicular tissues by
reversing the effects of IR. When ACA was applied together with IR, Caspase-3 (p<0.01), TOS (p<0.001), and OSI (p<0.001)
levels decreased significantly compared to the IR group. While NF-κB levels decreased insignificantly, TAS and Bcl-2 levels
increased insignificantly. Conclusions: ACA provides protective properties against testicular IR injury.

Keywords

Acacetin, apoptosis, ischemia reperfusion, oxidative stress, testis

Supporting Institution

Tubitak

Project Number

1919B012105612

Thanks

The authors thank Dr. Nurhan Akaras for her support in the histological examination. This study was supported by Tubitak 2209 project (2209/A, Project: 1919B012105612).

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