Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. catarrhalis and S. aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively

Aylin Eryılmaz; Bülent Ertuğrul; Yeşim Başal; Ali Bolaman; İmran Kurt Omurlu; Serhan Sakarya

doi:10.5152/ejra.2018.6

Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. catarrhalis and S. aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively

Abstract

Objective: The antibacterial characteristics of the platelet products against various microorganisms have been emphasized. Here we evaluated the antibacterial effectiveness of platelet rich plasma (PRP), platelet poor plasma (PPP), platelet-concentrated plasma (PCP), and noncoagulating platelet-derived factor concentrate (PFC) on Moraxella catarrhalis and Staphylococcus aureus in in vitro conditions. Material and Methods: Platelet rich plasma (PRP), PPP, PCP, and PFC were prepared. The standard strains of M. catarrhalis and S. aureus were mixed with the platelet solutions in vitro. Following incubation, the growing colonies were counted. This was repeated thrice. Results: The efficacies of PRP and PPP were found to be present against both bacteria in all dilutions. While PCP was effective only in the 1:1 dilution. No effectiveness of PFC was identified in any of the dilutions. Conclusion: The determination of significant antibacterial effects of PRP and PPP against S. aureus and M. catarrhalis indicate that they can be used in addition to the management of acute and chronic sinusitis.

Keywords

References

Intravia J, Allen DA, Durant TJ, McCarthy MB, Russell R, Beitzel K, et al. .In vitro evaluation of the anti-bacterial effect of two preparations of platelet rich plasma compared with cefazolinand whole blood. Muscles Ligaments Tendons J 2014; 4: 79-84.
Drago L, Bortolin M, Vassena C, Romanò CL, Taschieri S, Del Fabbro M. Plasma components and platelet activation are essential for the antimicrobial properties of autologous platelet-rich plasma: an in vitro study. PLoS One 2014; 9(9): doi: 10.1371/journal.pone.0107813.
Drago L, Bortolin M, Vassena C, Taschieri S, Del Fabbro M. Antimicrobial activity of pure platelet-rich plasma against microorganisms isolated from oralcavity. BMC Microbiol 2013; 13: 47.
Anitua E, Alonso R, Girbau C, Aguirre JJ, Muruzabal F, Orive G. Antibacterial effect of plasma rich in growth factors (PRGF®-Endoret®) against Staphylococcus aureus and Staphylococcus epidermidis strains. Clin Exp Dermatol 2012; 37: 652-7.
Chen L, Wang C, Liu H, Liu G, Ran X. Antibacterial effect of autologous platelet-rich gel derived from subjects with diabetic dermal ulcers in vitro. J Diabetes Res 2013; doi: 10.1155/2013/269527.
Burnouf T, Chou ML, Wu YW, Su CY, Lee LW. Antimicrobial activity of platelet (PLT)-poor plasma, PLT-rich plasma, PLT gel, and solvent/detergent-treated PLT lysate biomaterials against wound bacteria. Transfusion 2013; 53: 138-46.
Lee CW, Lee BJ, Yoo SH, Yi JS. Relationship between positive bacterial culture in maxillary sinus and surgical outcomes in chronic rhinosinusitis with nasal polyps. Auris Nasus Larynx 2014; 41: 446-9.
Tohidnezhad M, Varoga D, Wruck CJ, Podschun R, Sachweh BH, BornemannJ, et al. Platelets display potent antimicrobial activity and release human beta-defensin 2. Platelets 2012; 23: 217-23.

Yeaman MR. The role of platelets in antimicrobial host defense. Clin Infect Dis 1997; 25: 951-68.
Yeaman MR, Puentes SM, Norman DC, Bayer AS. Partial characterization and staphylocidal activity of thrombin-induced platelet microbicidal protein. Infect Immun 1992; 60: 1202-9.
Yeaman MR, Ibrahim AS, Edwards JE Jr, Bayer AS, Ghannoum MA. Thrombin-induced rabbit platelet microbicidal protein is fungicidal in vitro. Antimicrob Agents Chemother 1993; 37: 546-53.
Różalski MI, Micota B, Sadowska B, Paszkiewicz M, Więckowska-Szakiel M, Różalska B. Antimicrobial/anti-biofilm activity of expired blood platelets and their released products. PostepyHig Med Dosw 2013; 67: 321-5.
Foreman A, Psaltis AJ, Tan LW, Wormald PJ. Characterization of bacterial and fungal biofilms in chronic rhinosinusitis. Am J Rhinol Allergy 2009; 23: 556-61.
Stephenson M, Mfuna L, Dowd SE, Wolcott RD, Barbeau J, Poisson M, et al. Molecular characterization of the polymicrobial flora in chronic rhinosinusitis. J Otolaryngol Head Neck Surg 2010; 39: 182–7.
Garzoni C, Francois P, Huyghe A, Couzinet S, Tapparel C, CharbonnierY, et al. A global view of Staphylococcus aureus whole genome expression upon internalization in human epithelial cells. BMC Genomics 2007; 8: 171.
Zhang N, Gevaert P, van Zele T, Perez-Novo C, Patou J, Holtappels G, et al. An update on the impact of Staphylococcus aureusenterotoxins in chronic sinusitis with nasal polyposis. Rhinology 2005; 43: 162-8.
Payne SC, Benninger MS. Staphylococcus aureus is a major pathogen in acute bacterialrhinosinusitis: a meta-analysis. Clin Infect Dis 2007; 45: 121-7.
Fung CP, Yeo SF, Livermore DM. Susceptibility of Moraxella catarrhalisisolates to beta-lactam antibiotics in relation to beta-lactamase pattern. J Antimicrob Chemother 1994; 33: 215-22.
Araki J, Jona M, Eto H, Aoi N, Kato H, Suga H, et al. Optimized preparation method of platelet-concentrated plasma and noncoagulating platelet-derived factor concentrates: maximization of platelet concentration and removal of fibrinogen. Tissue Eng Part C Methods 2012; 18: 176-85.
Tang YQ, Yeaman MR, Selsted ME. Antimicrobial peptides from human platelets. Infect Immun 2002; 70: 6524-33.
Yang LC, Hu SW, Yan M, Yang JJ, Tsou SH, Lin YY. Antimicrobial activity of platelet-rich plasma and other plasma preparations against periodontal pathogens. J Periodontol 2015; 86: 310-8.
Intravia J, Allen DA, Durant TJ, McCarthy MB, Russell R, Beitzel K, et al. In vitro evaluation of the antibacterial effect of two preparations of platelet rich plasma compared with cefazolin and whole blood. Muscles Ligaments Tendons J 2014; 4: 79-84.
Mariani E, Canella V, Berlingeri A, Bielli A, Cattini L, Landini MP, et al. Leukocyte presence does not increasemicrobicidal activity of Platelet-rich Plasma in vitro. BMC Microbiol 2015; 15: 149.

Details

Primary Language

English

Subjects

Otorhinolaryngology

Journal Section

Research Article

Authors

Aylin Eryılmaz
0000-0002-1417-7267
Türkiye

Bülent Ertuğrul
0000-0002-6372-6680
Türkiye

Yeşim Başal
0000-0001-9002-0619
Türkiye

Ali Bolaman
0000-0003-0651-5462
Türkiye

İmran Kurt Omurlu
0000-0003-2887-6656
Türkiye

Serhan Sakarya This is me
0000-0001-5252-9211
Türkiye

Publication Date

August 1, 2018

Submission Date

May 3, 2018

Acceptance Date

July 2, 2018

Published in Issue

Year 2018 Volume: 1 Number: 2

DOI

https://doi.org/10.5152/ejra.2018.6

IZ

https://izlik.org/JA82GK35PB

Cite

RIS / Bibtex

APA

Eryılmaz, A., Ertuğrul, B., Başal, Y., Bolaman, A., Kurt Omurlu, İ., & Sakarya, S. (2018). Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. catarrhalis and S. aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively. European Journal of Rhinology and Allergy, 1(2), 27-31. https://doi.org/10.5152/ejra.2018.6

AMA

1.Eryılmaz A, Ertuğrul B, Başal Y, Bolaman A, Kurt Omurlu İ, Sakarya S. Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. catarrhalis and S. aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively. Eur J Rhinol Allergy. 2018;1(2):27-31. doi:10.5152/ejra.2018.6

Chicago

Eryılmaz, Aylin, Bülent Ertuğrul, Yeşim Başal, Ali Bolaman, İmran Kurt Omurlu, and Serhan Sakarya. 2018. “Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. Catarrhalis and S. Aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively”. European Journal of Rhinology and Allergy 1 (2): 27-31. https://doi.org/10.5152/ejra.2018.6.

EndNote

Eryılmaz A, Ertuğrul B, Başal Y, Bolaman A, Kurt Omurlu İ, Sakarya S (August 1, 2018) Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. catarrhalis and S. aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively. European Journal of Rhinology and Allergy 1 2 27–31.

IEEE

[1]A. Eryılmaz, B. Ertuğrul, Y. Başal, A. Bolaman, İ. Kurt Omurlu, and S. Sakarya, “Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. catarrhalis and S. aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively”, Eur J Rhinol Allergy, vol. 1, no. 2, pp. 27–31, Aug. 2018, doi: 10.5152/ejra.2018.6.

ISNAD

Eryılmaz, Aylin - Ertuğrul, Bülent - Başal, Yeşim - Bolaman, Ali - Kurt Omurlu, İmran - Sakarya, Serhan. “Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. Catarrhalis and S. Aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively”. European Journal of Rhinology and Allergy 1/2 (August 1, 2018): 27-31. https://doi.org/10.5152/ejra.2018.6.

JAMA

1.Eryılmaz A, Ertuğrul B, Başal Y, Bolaman A, Kurt Omurlu İ, Sakarya S. Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. catarrhalis and S. aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively. Eur J Rhinol Allergy. 2018;1:27–31.

MLA

Eryılmaz, Aylin, et al. “Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. Catarrhalis and S. Aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively”. European Journal of Rhinology and Allergy, vol. 1, no. 2, Aug. 2018, pp. 27-31, doi:10.5152/ejra.2018.6.

Vancouver

1.Aylin Eryılmaz, Bülent Ertuğrul, Yeşim Başal, Ali Bolaman, İmran Kurt Omurlu, Serhan Sakarya. Antibacterial Effectiveness of Platelet Products (PRP, PPP, PCP, and PFC) on M. catarrhalis and S. aureus, a Causative Agent of Acute and Chronic Sinusitis, Respectively. Eur J Rhinol Allergy. 2018 Aug. 1;1(2):27-31. doi:10.5152/ejra.2018.6