Isolation, characterization and screening of PGPR capable of providing relief in salinity stress

Abstract

Environmental stresses such as drought, temperature, salinity, air pollution, heavy metals, pesticides, and soil pH are major limiting factors in crop production because they affect almost all plant functions. Soil salinization is a serious stress condition causing major problem for crop productivity. To combat this salinity stress, Plant growth promoting rhizobacteria (PGPR) is considered as innovative, effective and ecofriendly approach. Plant growth promoting rhizobacteria (PGPR) have various direct and indirect mechanisms which can be correlated with their ability to form biofilms, chemotaxis, and the production of exopolysaccharide, indole-3-acetic acids (IAA) and aminocyclopropane-1- carboxylate (ACC) deaminase Investigations on the interaction of PGPR with other microbes and their effect on the physiological response of crop plants under different soil salinity regimes are still at an incipient stage. An experiment was conducted to investigate the effect of PGPR on lowering down the salt stress. Treatments were control (T₁), Salt tolerant isolate KH-1 (T₂), Salt tolerant isolate KH-2 (T₃), Salt tolerant isolate KH-3 (T₄), PGPR-I (Pseudimonas) (T₅), PGPR-II (Azotobacter) (T₆). Rice was sown under saline conditions at Soil Salinity Research Institute, Pindi Bhattian. With the inoculation of salt tolerant PGPR, plant growth and yield was improved. Result showed significant increase in plant height, biomass and yield over control. Inoculation of salt tolerant isolate KH-2 produced maximum grain yield in rice (4267 kg/ha) followed by PGPR-II and it was statistically significant from all other treatments along with control. It is concluded that with the application of salt tolerant isolate (KH-2), there is significant increase in rice production.

Keywords

• PGPR,abiotic stresses,Azotobacter,Pseudomonas,auxin production

References

1. Ahmed, H.M.I., Farag, M.M.A., 2011. Alleviation of salinity stress in lettuce during germination by seed priming. Journal of Plant Production - Mansoura University 2(5): 725–737
2. Amin, U.S.M., Biswas, S., Elias, S.M., Razzaque, S., Haque, T., Malo, R., Seraj, Z.I., 2016. Enhanced salt tolerance conferred by the complete 2.3 kb cDNA of the rice vacuolar Na+/H+ antiporter gene compared to 1.9 kb coding region with 5′ UTR in transgenic lines of rice. Frontiers in Plant Science 7: 14.
3. Arshad, M., Frankenberger Jr., W.T., 1998. Plant growth-regulating substances in the rhizosphere: Microbial production and functions. Advances in Agronomy 62: 45-151.
4. Bacilio, M., Rodriguez, H., Moreno, M., Hernandez, J.P., Bashan, Y., 2004. Mitigation of salt stress in wheat seedlings by a gfp-tagged Azospirillum lipoferum. Biology and Fertility of Soils 40(3) 188–193.
5. Chaudhary, M.R. 2001. Gypsum efficiency in the amelioration of saline sodic/sodic soils. International Journal of Agriculture and Biology 3(3):276-280.
6. Choudhary, D.K., Kasotia, A., Jain, S., Vaishnav, A., Kumari, S., Sharma, K.P., Varma, A., 2015. Bacterial-mediated tolerance and resistance to plants under abiotic and biotic stresses. Journal of Plant Growth Regulation 35(1): 276–300.
7. da Costa, M., H. Santos and E. Galinski. 1998. An overview of the role and diversity of compatible solutes in Bacteria and Archaea. In: Biotechnology of Extremophiles. Antranikian, G. (Ed.). Springer, Volume 61, pp. 117–153.
8. Dodd, I.C., Pérez-Alfocea, F., 2012. Microbial amelioration of crop salinity stress Journal of Experimental Botany 63(9): 3415–3428.

9. FAO, 2008. Global network on integrated soil management for sustainable use of salt-affected soils. Food and Agriculture Organization of the United Nations, Land and Plant Nutrition Management Service, Rome, Italy.
10. Flowers, T.J., 1999. Salinisation and horticultural production. Scientia Horticulturae (Amsterdam) 78: 1–4.
11. Flowers, T.J., Flowers, S.A., 2005. Why does salinity pose such a difficult problem for plant breeders? Agricultural Water Management 78(1-2): 15–24.
12. Glick, B.R, Penrose, D.M., Li, J., 1998. A model for the lowering of plant ethylene concentrations by plant growth-promoting bacteria. Journal of Theoretical Biology 190(1): 63–68.
13. Glick, B.R., 2010. Using soil bacteria to facilitate phytoremediation. Biotechnology Advances 28(3): 367–374.
14. Glick, B.R., 2014. Bacteria with ACC deaminase can promote plant growth and help to feed the world. Microbiological Research 169(1):30–39.
15. Harrison, M.J., Dewbre, G.R., Liu, J., 2002. A phosphate transporter from medicago truncatula ınvolved in the acquisition of phosphate released by arbuscular mycorrhizal fungi. The Plant Cell 14: 2413-2429.
16. Hasegawa, P.M., Bressan, R.A., Zhu, J.K., Bohnert, H.J., 2000. Plant cellular and molecular responses to high salinity. Annual Review of Plant Physiology and Plant Molecular Biology 51: 463-499.
17. Hichem, H., El Naceur, A., Mounir, D., 2009. Effects of salt stress on photosynthesis, PSII photochemistry and thermal energy dissipation in leaves of two corn (Zea mays L.) varieties. Photosynthetica 47(4): 517-526.
18. Huang, G.T., Ma, S.L., Bai, L.P., Zhang, L., Ma, H., Jia, P., Liu, J., Zhong, M., Guo, Z.F., 2012. Signal transduction during cold, salt, and drought stresses in plants. Molecular Biology Reports 39(2): 969–987.
19. Jeong, J.S., Kim, Y.S., Baek, K.H., Jung, H., Ha, S.H., Do Choi, Y., Kim, M., Reuzeau, C., Kim, J.K., 2010. Root-specific expression of OsNAC10 improves drought tolerance and grain yield in rice under field drought conditions. Plant Physiology 153(1): 185–197.
20. Jouyban, Z., 2012. The effects of salt stress on plant growth. Technical Journal of Engineering and Applied Sciences 2(1): 7-10.
21. Khan, K., Agarwal, P., Shanware, A., Sane, V.A., 2015. Heterologous expression of two Jatropha Aquaporins imparts drought and salt tolerance and ımproves seed viability in transgenic Arabidopsis thaliana. PLoS One 10(6): e0128866.
22. Kloepper, J.W., Lifshitz, R., Zablotowicz, R.M., 1989. Free-living bacterial inocula for enhancing crop productivity. Trends in Biotechnology 7(2): 39-43.
23. Liu, X.M., Zhang, H., 2015. The effects of bacterial volatile emissions on plant abiotic stress tolerance. Frontiers in Plant Science 6: 774.
24. Marschner, H., 1995. Mineral nutrition of higher plants. London: Academic Press. 889p.
25. Munns, R., Tester, M., 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology 59: 651–681.
26. Nawaz, S. Akhtar, N. Aslam, M. Qureshi, R.H., Akhtar, J., 2002. Anatomical, morphological and physiological changes in sunflower varieties because of NaCl salinity. Pakistan Journal of Soil Science 21:87-93.
27. Neto, A.A.D., Prisco, J.T., Enéas-Filho, J., Abreu, C.E.B., Gomes-Filho, E., 2006. Effect of salt stress on antioxidative enzymes and lipid peroxidation in leaves and roots of salt-tolerant and salt-sensitive maize genotypes. Environmental and Experimental Botany 56(1): 87-94.
28. Nounjan, N., Nghia, P.T., Theerakulpisut, P., 2012. Exogenous proline and trehalose promote recovery of rice seedlings from salt-stress and differentially modulate antioxidant enzymes and expression of related genes. Journal of Plant Physiology 169(6): 596–604.
29. Parida, A.K., Das, A.B., 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology and Environmental Safety 60(3): 324–349.
30. Qureshi, M.A., Shakir, M.A., Iqbal, A., Akhtar, N., Khan, A., 2011. Co-inoculation of phosphate solubilizing bacteria and rhizobia for improving growth and yield of mungbean (Vigna radiata L.). Journal of Animal and Plant Sciences 21(3): 491-497.
31. Rahman, M.A., Thomson, M.J., Shah-E-Alam, M., de Ocampo, M., Egdane, J., Ismail, A.M., 2016. Exploring novel genetic sources of salinity tolerance in rice through molecular and physiological characterization. Annals of Botany 117(6): 1083–1097.
32. Rani, M.U., Arundhathi, A., Reddy, G., 2012. Screening of rhizobacteria containing plant growth promoting (PGPR) traits in rhizosphere soils and their role in enhancing growth of pigeon pea. African Journal of Biotechnology 11(32): 8085-8091.
33. Rengasamy, P., 2006. World salinization with emphasis on Australia. Journal of Experimental Botany 57(5): 1017–1023.
34. Sarwar, M., Arshad, M., Martins, D.A., Frankenberger Jr, W.T., 1992. Tryptophan-dependent biosynthesis of auxins in soil. Plant and Soil 147(2): 207-215.
35. Sheldon, A., Menzies, N.W., Bing, S.H., Dalal, R.C., 2004. The effect of salinity on plant available water. Supersoil 2004: 3rd Australian/New Zealand Soils Conference. 5 – 9 December 2004. Sydney, Australia. Available at [access date: 19.02.2019]: http://www.regional.org.au/au/asssi/supersoil2004/s6/poster/1523_sheldona.htm
36. Singh, A.L., Hariprassanal, K., Solanki, R.M., 2008. Screening and selection of groundnut genotypes for tolerance of soil salinity. Australian Journal of Crop Science 1(3):69-77.
37. Steel, R.G.D., Torrie, J.H., Dickey, D.A., 1997. Principles and procedures of statistics: a biometrical approach. 3rd ed. McGraw-Hill, New York, USA. 666p.
38. Tavakkoli, E., Rengasamy, P., McDonald, G.K., 2010. High concentrations of Na+ and Cl− ions in soil solution have simultaneous detrimental effects on growth of fava bean under salinity stress. Journal of Experimental Botany 61(15): 4449–4459.
39. Tilak, K.V.B.R., Ranganayaki, N., Pal, K.K., De, R., Saxena, A.K., Nautiyal, C.S., Mittal, S., Tripathi, A.K., Johri, B.N., 2005. Diversity of plant growth and soil health-supporting bacteria. Current Science 89(1): 136-150.
40. Wang, M.Y., Siddiqi, M.Y., Ruth, T.J., Glass, A.D.M., 1993. Ammonium uptake by rice roots (I. Fluxes and subcellular distribution of 13NH4+). Plant Physiology 103: 1249–1258.
41. Yildirim, E., Taylor, A.G., Spittler, T.D., 2006. Ameliorative effects of biological treatments on growth of squash plants under salt stress. Scientia Horticulturae 111(1): 1-6.
42. Zhang, J.L., Flowers, T.J., Wang, S.M., 2010. Mechanisms of sodium uptake by roots of higher plants. Plant and Soil 326: 45–60.
43. Zhu, J.K., 2001. Plant salt tolerance. Trends in Plant Science 6(2): 66-71.