Research Article
BibTex RIS Cite

Year 2025, Volume: 11 Issue: 2 , 60 - 67 , 31.07.2025

Selcen Doğan Cansu Şimşek , Murat X Murat Selçuk Hasan Pinar Nedim Mutlu Ş. Şebnem Ellialtioğlu

https://izlik.org/JA64JH74PZ

Abstract

References

  • Anonymous, (2024). Bitkisel üretim istatistikleri. https:// biruni.tuik.gov.tr/medas/?locale=tr Access Date: 16.06.2025 (in Turkish)
  • Baktemur G, Keleş D, Kara E, Yıldız S & Taşkın H, (2022). Effects of genotype and nutrient medium on obtaining haploid plants through ovary culture in cucumber. Molecular Biology Reports, 49:5451- 5458. https://doi.org/10.1007/s11033-022-07238-y
  • Beharav A & Cohen Y, (1995). Effect of kinetin and GA3 on in vitro ovule embryo culture of Cucumis melo L. Plant Growth Regulation, 16(3):267-269. https://doi.org/10.1007/BF00024785
  • Chambonnet D & Vaulx RD, (1985). Obtention of embryos and plants from in vitro culture of unfertilized ovules of Cucurbita pepo. Cucurbit Genetics Cooperative, 8:66
  • Çağlar G & Şensoy S, (2021). Hıyar ıslahı. In K. Abak, A. Balkaya, Ş. Ş. Ellialtıoğlu & E. Düzyaman (Eds.), Sebze Islahı. Cilt II: Cucurbitaceae (Kabakgiller) (ss. 87-201). BİSAB Yayınları, Gece Kitaplığı Yayınevi (in Turkish) Çetinkaya E, (2015). Farklı besi ortamı kombinasyonlarının bazı hıyar (Cucumis sativus L.) genotiplerinde gynogenesis yolu ile embriyo ve haploid bitki oluşumu üzerine etkisi [Yüksek lisans tezi, Akdeniz Üniversitesi]. Antalya (in Turkish)
  • Diao WP, Jia YY, Song H, Zhang XQ, Lou QF & Chen JF, (2009). Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenerants using SSR markers. Scientia Horticulturae, 119(3):246-251. https://doi. org/10.1016/j.scienta.2008.08.016
  • Domblides E, Shmykova N, Khimich G, Korotseva I, Kan L, Domblides A, Pivovarov V & Soldatenko A, (2020). Production of doubled haploid plants of Cucurbitaceae family crops through unpollinated ovule culture in vitro. In VI International Symposium on Cucurbits (Vol. 1294, pp. 19-28) https://doi.org/10.17660/ActaHortic.2020.1294.4
  • Dong YQ, Zhao WX, Li XH, Liu XC, Gao NN, Huang JH, Wang WY, Xu XL & Tang ZH, (2016). Androgenesis, gynogenesis, and parthenogenesis haploids in cucurbit species. Plant Cell Reports, 35, 1991-2019. https://doi.org/10.1007/s00299- 016-2018-7
  • Dumas de Vaulx R & Chambonnet D, (1986). Obtention of embryos and plants from in vitro culture of unfertilized ovules of Cucurbita pepo. In W. Gruyter (Ed.), Genetic Manipulation in Plant Breeding (pp. 295-297). De Gruyter.
  • FAOSTAT, (2023). http://www.fao.org/faostat/en/#data/ QC (Access Date: 16.06.2025)
  • Ficcadenti N, Sestili S, Annibali S, Marco MD & Schiavi M, (1999). In vitro gynogenesis to induce haploid plants in melon (Cucumis melo L.). Journal of Genetics and Breeding, 53(3):255-257.
  • Gémes Juhász A, Balogh P, Ferenczy A & Kristo´f Z, (2002). Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.). Plant Cell Reports, 21(2):105-111. https://doi.org/10.1007/s00299-002-0482-8
  • Gémes Juhász A & Jakše M, (2005). Haploids in the improvement of miscellaneous crop species (Cucurbitaceae, Liliaceae, Asparagaceae, Chenopodiaceae, Araceae and Umbelliferae). In C. E. Don Palmer, W. A. Keller, & K. J. Kasha (Eds.), Biotechnology in Agriculture and Forestry: Haploids in Crop Improvement II (pp. 259-278). Springer
  • Gémesné Juhász A, Venczel G & Balogh P, (1997). Haploid plant induction in zucchini (Cucurbita pepo L. convar. giromontiina Duch) and in cucumber (Cucumis sativus L.) lines through in vitro gynogenesis. Acta Horticulturae, 447, 623- 625
  • Günay A, (2005). Sebze Yetiştiriciliği (Cilt II). Meta Basımevi (in Turkish)
  • Kwack SN & Fujieda K, (1988). Somatic embryogenesis in cultured unfertilized ovules of Cucurbita moschata. Journal of the Japanese Society for Horticultural Science, 57(1):34-42. https://doi. org/10.2503/jjshs.57.34
  • Li JW, Si SW, Cheng JY, Li JX & Liu JQ, (2013). Thidiazuron and silver nitrate enhanced gynogenesis of unfertilized ovule cultures of Cucumis sativus. Biologia Plantarum, 57(1):164- 168. https://doi.org/10.1007/s10535-012-0269-x
  • Metwally EI, Moustafa S A, EI-Sawy BI & Shalaby TA, (1998a). Haploid plantlets derived by anther culture of Cucurbita pepo. Plant Cell, Tissue and Organ Culture, 52(3):171-176. https://doi. org/10.1023/A:1005908326663
  • Metwally EI, Moustafa SA, El-Sawy BI, Haroun SA & Shalaby TA, (1998b). Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo L. Plant Cell, Tissue and Organ Culture, 52(3):117-121. https://doi. org/10.1023/A:1005948809825
  • Min ZY, Li H, Zou T, Tong L, Cheng J & Sun XW, (2016). Studies of in vitro culture and plant regeneration of unfertilized ovary of pumpkin. Chinese Bulletin of Botany, 51(1):74-80. https:// doi.org/10.11983/CBB15004
  • Moqbeli E, Peyvast GH, Hamidoghli Y & Olfati JA, (2013). In vitro cucumber haploid line generation in several new cultivars. Asia-Pacific Journal of Molecular Biology and Biotechnology, 21(1): 18- 25
  • Murashige T & Skoog F, (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3):473-497
  • Nyirahabimana F & Solmaz İ, (2021). Haploid plant production technology in cucumber (Cucumis sativus L.). In M. Tutuncu (Ed.), Proceedings Book. 10th Int. Mol. Biol. & Biotech. Congress (pp. 28-37). Online
  • Nyirahabimana F & Solmaz İ, (2024). Haploid induction through ovary culture in cucumber. In Vitro Cellular & Developmental Biology-Plant, 60(1):122-130. https://doi.org/10.1007/s11627- 023-10406-9
  • Özsan T, Gozen V & Onus A, (2017). Cucumber gynogenesis: Effects of 8 different media on embryo and plant formation. International Journal of Agriculture Innovations and Research, 6(2):419- 422
  • Paris HS, Daunay MC & Janick J, (2012). Occidental diffusion of cucumber (Cucumis sativus) 500- 1300 CE: Two routes to Europe. Annals of Botany, 109(1):117-126. https://doi.org/10.1093/aob/ mcr281
  • Pınar H, Toprak S, Hancı F & Şimsek D, (2021). Effects of genotypic variation and some new protocols on gynogenesis efficiency of cucumber. Fresenius Environmental Bulletin, 30(5):4763-4770
  • Plapung P, Khamsukdee S, Potapohn N & Smitamana P, (2014). Screening for cucumber mosaic resistant lines from the ovule culture derived double haploid cucumbers. American Journal of Agricultural and Biological Sciences, 9(3):261-269. https://doi. org/10.3844/ajabssp.2014.261.269
  • Przyborowski J & Nlemirowicz‐Szgzytt K, (1994). Main factors affecting cucumber (Cucumis sativus L.) haploid embryo development and haploid plant characteristics. Plant Breeding, 112(1):70- 75. https://doi.org/10.1111/j.1439-0523.1994. tb01278.x
  • Rakha MT, Metwally EI, Moustafa SA, Etman AA & Dewir YH, (2012). Evaluation of regenerated strains from six ‘Cucurbita’ interspecific hybrids obtained through anther and ovule ‘in vitro’ cultures. Australian Journal of Crop Science, 6(1):23-30
  • Renner SS, Schaefer H & Kocyan A, (2007). Phylogenetics of Cucumis (Cucurbitaceae): Cucumber (C. sativus) belongs in an Asian/ Australian clade far from melon (C. melo). BMC Evolutionary Biology, 7(1):58. https://doi. org/10.1186/1471-2148-7-58
  • Renner SS & Schaefer H, (2008). Phylogenetics of Cucumis (Cucurbitaceae) as understood in 2008. In M. Pitrat (Ed.), Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae (pp. 53-58). Avignon, France
  • Shalaby TA, (2007). Factors affecting haploid induction through in vitro gynogenesis in summer squash (Cucurbita pepo L.). Scientia Horticulturae, 115(1):1-6. https://doi.org/10.1016/j. scienta.2007.07.008
  • Sun SR, Zhai QH, Hu JB, Chen JF & Zhang P, (2009). Effects of several physiological factors on embryo formation in unpollinated ovary culture of pumpkin (Cucurbita moschata Duch. Ex Poiret). Plant Physiology Communications, 45(10):977- 980
  • Suprunova T & Shmykova N, (2008). In vitro induction of haploid plants in unpollinated ovules, anther and microspore culture of Cucumis sativus. In M. Pitrat (Ed.), Proceedings of the IXth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae (pp. 371-374). Avignon, France
  • Staub JE, Robbins MD & Wehner TC, (2008). Cucumber. In J. Prohens & F. Nuez (Eds.), Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae, Handbook of Plant Breeding (Vol. 1, pp. 241-282). Springer
  • Tantasawat PA, Sorntip A, Poolsawat O, Chaowiset W & Pornbungkerd P, (2015). Evaluation of factors affecting embryo-like structure and callus formation in unpollinated ovary culture of cucumber (Cucumis sativus). International Journal of Agriculture and Biology, 17(3):613-618. https:// doi.org/10.17957/IJAB.17.3.14.257
  • Xie M, Qin LY, Pan JS, He HL, Wu AZ & Cai R, (2005). Flower morphogenesis and microspore development versus anther culture of cucumber. Acta Botanica Boreali-Occidentalia Sinica, 25(6):1096-1100

Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures

Year 2025, Volume: 11 Issue: 2 , 60 - 67 , 31.07.2025

Selcen Doğan Cansu Şimşek , Murat X Murat Selçuk Hasan Pinar Nedim Mutlu Ş. Şebnem Ellialtioğlu

https://izlik.org/JA64JH74PZ

Abstract

Haploids and doubled haploids play a crucial role in the process of plant breeding. Ovary culture has proven to be an
effective method for generating double haploid (DH) plants in cucumbers. The objective of this study was to optimize
culture media conditions for gynogenesis induction in vitro to broaden morphogenetic possibilities and accelerate the
creation of homozygous lines. The effects of seven different induction culture media on embryo and plant development
in the ovary culture of Beith Alpha-type and Silor-type cucumber (Cucumis sativus L.) were investigated. Ovary culture
experiments were conducted during the fall and spring seasons with 26 Beith Alpha-type and 11 Silor-type cucumber
genotypes at the F2 and F3 generations. The results revealed that the most successful outcomes were obtained from
genotypes coded HTB20, HTB24 and HTB25 of the Beith Alpha-type and HTS4 and HTS5 of the Silor-type. The findings
also demonstrated that explants cultured in the autumn season showed higher regeneration efficiency compared to those
cultured in spring. Overall when compared to Silor types, Beith Alpha types exhibited a more favorable response in terms
of plant regeneration and embryo development, depending on genotype.

Keywords

Gynogenesis medium compositions haploid Beith Alpha Silor-Type

References

  • Anonymous, (2024). Bitkisel üretim istatistikleri. https:// biruni.tuik.gov.tr/medas/?locale=tr Access Date: 16.06.2025 (in Turkish)
  • Baktemur G, Keleş D, Kara E, Yıldız S & Taşkın H, (2022). Effects of genotype and nutrient medium on obtaining haploid plants through ovary culture in cucumber. Molecular Biology Reports, 49:5451- 5458. https://doi.org/10.1007/s11033-022-07238-y
  • Beharav A & Cohen Y, (1995). Effect of kinetin and GA3 on in vitro ovule embryo culture of Cucumis melo L. Plant Growth Regulation, 16(3):267-269. https://doi.org/10.1007/BF00024785
  • Chambonnet D & Vaulx RD, (1985). Obtention of embryos and plants from in vitro culture of unfertilized ovules of Cucurbita pepo. Cucurbit Genetics Cooperative, 8:66
  • Çağlar G & Şensoy S, (2021). Hıyar ıslahı. In K. Abak, A. Balkaya, Ş. Ş. Ellialtıoğlu & E. Düzyaman (Eds.), Sebze Islahı. Cilt II: Cucurbitaceae (Kabakgiller) (ss. 87-201). BİSAB Yayınları, Gece Kitaplığı Yayınevi (in Turkish) Çetinkaya E, (2015). Farklı besi ortamı kombinasyonlarının bazı hıyar (Cucumis sativus L.) genotiplerinde gynogenesis yolu ile embriyo ve haploid bitki oluşumu üzerine etkisi [Yüksek lisans tezi, Akdeniz Üniversitesi]. Antalya (in Turkish)
  • Diao WP, Jia YY, Song H, Zhang XQ, Lou QF & Chen JF, (2009). Efficient embryo induction in cucumber ovary culture and homozygous identification of the regenerants using SSR markers. Scientia Horticulturae, 119(3):246-251. https://doi. org/10.1016/j.scienta.2008.08.016
  • Domblides E, Shmykova N, Khimich G, Korotseva I, Kan L, Domblides A, Pivovarov V & Soldatenko A, (2020). Production of doubled haploid plants of Cucurbitaceae family crops through unpollinated ovule culture in vitro. In VI International Symposium on Cucurbits (Vol. 1294, pp. 19-28) https://doi.org/10.17660/ActaHortic.2020.1294.4
  • Dong YQ, Zhao WX, Li XH, Liu XC, Gao NN, Huang JH, Wang WY, Xu XL & Tang ZH, (2016). Androgenesis, gynogenesis, and parthenogenesis haploids in cucurbit species. Plant Cell Reports, 35, 1991-2019. https://doi.org/10.1007/s00299- 016-2018-7
  • Dumas de Vaulx R & Chambonnet D, (1986). Obtention of embryos and plants from in vitro culture of unfertilized ovules of Cucurbita pepo. In W. Gruyter (Ed.), Genetic Manipulation in Plant Breeding (pp. 295-297). De Gruyter.
  • FAOSTAT, (2023). http://www.fao.org/faostat/en/#data/ QC (Access Date: 16.06.2025)
  • Ficcadenti N, Sestili S, Annibali S, Marco MD & Schiavi M, (1999). In vitro gynogenesis to induce haploid plants in melon (Cucumis melo L.). Journal of Genetics and Breeding, 53(3):255-257.
  • Gémes Juhász A, Balogh P, Ferenczy A & Kristo´f Z, (2002). Effect of optimal stage of female gametophyte and heat treatment on in vitro gynogenesis induction in cucumber (Cucumis sativus L.). Plant Cell Reports, 21(2):105-111. https://doi.org/10.1007/s00299-002-0482-8
  • Gémes Juhász A & Jakše M, (2005). Haploids in the improvement of miscellaneous crop species (Cucurbitaceae, Liliaceae, Asparagaceae, Chenopodiaceae, Araceae and Umbelliferae). In C. E. Don Palmer, W. A. Keller, & K. J. Kasha (Eds.), Biotechnology in Agriculture and Forestry: Haploids in Crop Improvement II (pp. 259-278). Springer
  • Gémesné Juhász A, Venczel G & Balogh P, (1997). Haploid plant induction in zucchini (Cucurbita pepo L. convar. giromontiina Duch) and in cucumber (Cucumis sativus L.) lines through in vitro gynogenesis. Acta Horticulturae, 447, 623- 625
  • Günay A, (2005). Sebze Yetiştiriciliği (Cilt II). Meta Basımevi (in Turkish)
  • Kwack SN & Fujieda K, (1988). Somatic embryogenesis in cultured unfertilized ovules of Cucurbita moschata. Journal of the Japanese Society for Horticultural Science, 57(1):34-42. https://doi. org/10.2503/jjshs.57.34
  • Li JW, Si SW, Cheng JY, Li JX & Liu JQ, (2013). Thidiazuron and silver nitrate enhanced gynogenesis of unfertilized ovule cultures of Cucumis sativus. Biologia Plantarum, 57(1):164- 168. https://doi.org/10.1007/s10535-012-0269-x
  • Metwally EI, Moustafa S A, EI-Sawy BI & Shalaby TA, (1998a). Haploid plantlets derived by anther culture of Cucurbita pepo. Plant Cell, Tissue and Organ Culture, 52(3):171-176. https://doi. org/10.1023/A:1005908326663
  • Metwally EI, Moustafa SA, El-Sawy BI, Haroun SA & Shalaby TA, (1998b). Production of haploid plants from in vitro culture of unpollinated ovules of Cucurbita pepo L. Plant Cell, Tissue and Organ Culture, 52(3):117-121. https://doi. org/10.1023/A:1005948809825
  • Min ZY, Li H, Zou T, Tong L, Cheng J & Sun XW, (2016). Studies of in vitro culture and plant regeneration of unfertilized ovary of pumpkin. Chinese Bulletin of Botany, 51(1):74-80. https:// doi.org/10.11983/CBB15004
  • Moqbeli E, Peyvast GH, Hamidoghli Y & Olfati JA, (2013). In vitro cucumber haploid line generation in several new cultivars. Asia-Pacific Journal of Molecular Biology and Biotechnology, 21(1): 18- 25
  • Murashige T & Skoog F, (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3):473-497
  • Nyirahabimana F & Solmaz İ, (2021). Haploid plant production technology in cucumber (Cucumis sativus L.). In M. Tutuncu (Ed.), Proceedings Book. 10th Int. Mol. Biol. & Biotech. Congress (pp. 28-37). Online
  • Nyirahabimana F & Solmaz İ, (2024). Haploid induction through ovary culture in cucumber. In Vitro Cellular & Developmental Biology-Plant, 60(1):122-130. https://doi.org/10.1007/s11627- 023-10406-9
  • Özsan T, Gozen V & Onus A, (2017). Cucumber gynogenesis: Effects of 8 different media on embryo and plant formation. International Journal of Agriculture Innovations and Research, 6(2):419- 422
  • Paris HS, Daunay MC & Janick J, (2012). Occidental diffusion of cucumber (Cucumis sativus) 500- 1300 CE: Two routes to Europe. Annals of Botany, 109(1):117-126. https://doi.org/10.1093/aob/ mcr281
  • Pınar H, Toprak S, Hancı F & Şimsek D, (2021). Effects of genotypic variation and some new protocols on gynogenesis efficiency of cucumber. Fresenius Environmental Bulletin, 30(5):4763-4770
  • Plapung P, Khamsukdee S, Potapohn N & Smitamana P, (2014). Screening for cucumber mosaic resistant lines from the ovule culture derived double haploid cucumbers. American Journal of Agricultural and Biological Sciences, 9(3):261-269. https://doi. org/10.3844/ajabssp.2014.261.269
  • Przyborowski J & Nlemirowicz‐Szgzytt K, (1994). Main factors affecting cucumber (Cucumis sativus L.) haploid embryo development and haploid plant characteristics. Plant Breeding, 112(1):70- 75. https://doi.org/10.1111/j.1439-0523.1994. tb01278.x
  • Rakha MT, Metwally EI, Moustafa SA, Etman AA & Dewir YH, (2012). Evaluation of regenerated strains from six ‘Cucurbita’ interspecific hybrids obtained through anther and ovule ‘in vitro’ cultures. Australian Journal of Crop Science, 6(1):23-30
  • Renner SS, Schaefer H & Kocyan A, (2007). Phylogenetics of Cucumis (Cucurbitaceae): Cucumber (C. sativus) belongs in an Asian/ Australian clade far from melon (C. melo). BMC Evolutionary Biology, 7(1):58. https://doi. org/10.1186/1471-2148-7-58
  • Renner SS & Schaefer H, (2008). Phylogenetics of Cucumis (Cucurbitaceae) as understood in 2008. In M. Pitrat (Ed.), Cucurbitaceae 2008, Proceedings of the IXth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae (pp. 53-58). Avignon, France
  • Shalaby TA, (2007). Factors affecting haploid induction through in vitro gynogenesis in summer squash (Cucurbita pepo L.). Scientia Horticulturae, 115(1):1-6. https://doi.org/10.1016/j. scienta.2007.07.008
  • Sun SR, Zhai QH, Hu JB, Chen JF & Zhang P, (2009). Effects of several physiological factors on embryo formation in unpollinated ovary culture of pumpkin (Cucurbita moschata Duch. Ex Poiret). Plant Physiology Communications, 45(10):977- 980
  • Suprunova T & Shmykova N, (2008). In vitro induction of haploid plants in unpollinated ovules, anther and microspore culture of Cucumis sativus. In M. Pitrat (Ed.), Proceedings of the IXth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae (pp. 371-374). Avignon, France
  • Staub JE, Robbins MD & Wehner TC, (2008). Cucumber. In J. Prohens & F. Nuez (Eds.), Vegetables I: Asteraceae, Brassicaceae, Chenopodicaceae, and Cucurbitaceae, Handbook of Plant Breeding (Vol. 1, pp. 241-282). Springer
  • Tantasawat PA, Sorntip A, Poolsawat O, Chaowiset W & Pornbungkerd P, (2015). Evaluation of factors affecting embryo-like structure and callus formation in unpollinated ovary culture of cucumber (Cucumis sativus). International Journal of Agriculture and Biology, 17(3):613-618. https:// doi.org/10.17957/IJAB.17.3.14.257
  • Xie M, Qin LY, Pan JS, He HL, Wu AZ & Cai R, (2005). Flower morphogenesis and microspore development versus anther culture of cucumber. Acta Botanica Boreali-Occidentalia Sinica, 25(6):1096-1100
There are 38 citations in total.

Details

Primary Language English
Subjects Agronomy
Journal Section Research Article
Authors

Selcen Doğan This is me

Cansu Şimşek

Murat X Murat Selçuk This is me

Hasan Pinar This is me

Nedim Mutlu This is me

Ş. Şebnem Ellialtioğlu This is me

Submission Date April 27, 2025
Acceptance Date June 25, 2025
Publication Date July 31, 2025
IZ https://izlik.org/JA64JH74PZ
Published in Issue Year 2025 Volume: 11 Issue: 2

Cite

APA Doğan, S., Şimşek, C., Murat Selçuk, M. X., Pinar, H., Mutlu, N., & Ellialtioğlu, Ş. Ş. (2025). Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures. Ekin Journal of Crop Breeding and Genetics, 11(2), 60-67. https://izlik.org/JA64JH74PZ
AMA 1.Doğan S, Şimşek C, Murat Selçuk MX, Pinar H, Mutlu N, Ellialtioğlu ŞŞ. Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures. Ekin Journal. 2025;11(2):60-67. https://izlik.org/JA64JH74PZ
Chicago Doğan, Selcen, Cansu Şimşek, Murat X Murat Selçuk, Hasan Pinar, Nedim Mutlu, and Ş. Şebnem Ellialtioğlu. 2025. “Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis Sativus L.) Ovary Cultures”. Ekin Journal of Crop Breeding and Genetics 11 (2): 60-67. https://izlik.org/JA64JH74PZ.
EndNote Doğan S, Şimşek C, Murat Selçuk MX, Pinar H, Mutlu N, Ellialtioğlu ŞŞ (July 1, 2025) Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures. Ekin Journal of Crop Breeding and Genetics 11 2 60–67.
IEEE [1]S. Doğan, C. Şimşek, M. X. Murat Selçuk, H. Pinar, N. Mutlu, and Ş. Ş. Ellialtioğlu, “Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures”, Ekin Journal, vol. 11, no. 2, pp. 60–67, July 2025, [Online]. Available: https://izlik.org/JA64JH74PZ
ISNAD Doğan, Selcen - Şimşek, Cansu - Murat Selçuk, Murat X - Pinar, Hasan - Mutlu, Nedim - Ellialtioğlu, Ş. Şebnem. “Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis Sativus L.) Ovary Cultures”. Ekin Journal of Crop Breeding and Genetics 11/2 (July 1, 2025): 60-67. https://izlik.org/JA64JH74PZ.
JAMA 1.Doğan S, Şimşek C, Murat Selçuk MX, Pinar H, Mutlu N, Ellialtioğlu ŞŞ. Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures. Ekin Journal. 2025;11:60–67.
MLA Doğan, Selcen, et al. “Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis Sativus L.) Ovary Cultures”. Ekin Journal of Crop Breeding and Genetics, vol. 11, no. 2, July 2025, pp. 60-67, https://izlik.org/JA64JH74PZ.
Vancouver 1.Selcen Doğan, Cansu Şimşek, Murat X Murat Selçuk, Hasan Pinar, Nedim Mutlu, Ş. Şebnem Ellialtioğlu. Effects of Different Nutrient Media on Embryo Induction and Plant Regeneration in Cucumber (Cucumis sativus L.) Ovary Cultures. Ekin Journal [Internet]. 2025 Jul. 1;11(2):60-7. Available from: https://izlik.org/JA64JH74PZ