In Vitro Regeneration Studies in Turkish Tomato Cultivars

Abstract

Tomato is among the most extensively studied plant species in global breeding and genetic research. In recent years, CRISPR-Cas9 technology offers extensive opportunities for integrating traits difficult to transfer through classical breeding methods into qualified genotypes or for generating precise mutations. Furthermore, the regenerative capacity of the studied plant species or genotype in tissue culture is a prerequisite for advancing genetic studies. This study aimed to induce in vitro regeneration from cotyledon and hypocotyl explants of four different tomato varieties (PTK-254, PTK-273, Şekerkız, Yelpare 82) developed by Petektar Seed Co. Organogenesis was achieved in MS medium using different doses and combinations of the growth regulators TDZ, BAA, IAA, and Zeatin. In this study, where the interaction of nutrient medium and varieties was found to be important, the highest shoot formation was obtained from MS-1, MS-8, MS-5, and MS-6 media containing the Şekerkız variety. These combinations, in which more than 10 shoots were obtained from each explant, were followed by the Yelpare 82, PTK-254, and PTK-273 varieties cultured in MS-1 medium. The combination of 1.0 mg/L Zeatin and 0.1 mg/L IAA (MS-1) provided regeneration in all varieties, albeit to varying degrees. The use of TDZ alone at doses of 2 and 3 mg/L can also yield positive results depending on the variety. Developing shoots were sub-cultured and grown. For rooting, hormone-free MS medium was used, and tomato plantlets were transferred to peat pots and acclimatized. In this study, the in vitro regeneration system for advanced genetic applications was optimized on experimental material.

Keywords

• Tomatoes
• organogenesis
• Solanum lycopersicum L.
• Zeatin
• TDZ

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