Floral and Molecular Characterization of Gamma Rays Induced Mutants in Oil-Bearing Rose (Rosa damascena Mill.)

Abstract

This research was aimed to create the genetic variations for the selection of oil-bearing rose genotypes with the desirable floral features. For this reason, gamma rays at doses of 0, 100 and 200 Gy of radioactive Cobalt-60 were applied to oilbearing rose seeds to create genetic variations. Finally, the floral and molecular characterization of oil-bearing rose plants derived from non-irradiated and irradiated seeds were carried out. A total of 48 genotypes including 17 from control (0 Gy), 18 from M100 (100 Gy) and 12 from M200 (200 Gy) together with the parental species R. damascena were used as genetic materials. Genetic analysis was performed by using 20 SSR primer pairs. After the electrophoresis, the bands were displayed in TIF format with the aid of the Biolab UV Tech gel imaging system. The genotypes and mutants grown from irradiated and non-irradiated seeds were significantly different with flower colours from white to dark pink and petal numbers from 5 to 100. These results also showed that the seeds in the open-pollinated flowers of oil-bearing rose had mostly heterozygous allele genes governing the floral traits. The efficacy of the SSR primers used to identify mutations was different. RA003a and RA034a primers were found to be more effective in mutation screening in oil-bearing rose genome. It was understood that the major effects of the gamma-ray irradiation were on large-scale chromosomal breaks or deficiencies. In conclusion, oil-bearing rose seeds with or without gamma-ray irradiation would be a huge selection source to breed novel varieties.

Keywords

• Rosa damascena Mill.
• mutation breeding
• gamma-ray
• SSRs

References

1. Ağaoglu YS, Ergül A and Göktürk Baydar N (2000). Molecular analysis of genetic diversity oil rose (Rosa damascena Mill.) grown Isparta (Turkey) region. Biotechnol Biotech Eq. 14:16-18.
2. Alsemaan T, Albatal N, Baydar H and Almaarri K (2011). Genetic diversity and qualitative variation of Rosa damascena in Syria. Int J Agric Res. 6(5):429-436.
3. Anac O (1984). Gas chromatographic analysis on Turkish rose oil, absolute and concrete. Perfumer & Flavoris t 9:1-14.
4. Aycı F, Aydınlı M, Bozdemir OA and Tutas M (2005). Gas chromatographic inves tigation of rose concrete, absolute and solid residue. Flavour Frag J. 20:481-486.
5. Aydınlı M and Tutas M (2003). Production of rose absolute from rose concrete. Flavour Frag J. 18(1):26-31.
6. Babaei A, Tabaei-Aghdaei SR, Khosh-Khui M, Omidbaigi R, Naghavi MR, Esselink GD and Smulders MJM (2007). Microsatellite analysis of Damask rose (Rosa damascena Mill.) accessions from various regions in Iran reveals multiple genotypes. BMC Plant Biol. 7:12-19.
7. Başer, K.H.C. 1992. Turkish rose oil. Perfum Flavor. 17, 45-52.
8. Baydar H, Kazaz S and Erbaş S (2013). Yağ gülü (Rosa damascena Mill)’nde mutasyon ıslahı, Süleyman Demirel Üniversitesi Fen Bilimleri Ens titüsü Dergisi 17(2): 39-43 (in Turkish).

9. Baydar H, Erbaş S and Kazaz S (2016). Variations in floral characteris tics and scent composition and the breeding potential in seed-derived oil-bearing roses (Rosa damascena Mill.). Turkish J Agric Fores t. 40:560-569.
10. Bayrak A and Akgül A (1994). Volatile oil composition of Turkish rose (Rosa damascena). J Sci Food Agric. 64:441-448.
11. Bendahmane M, Dubois A, Raymond O and Bris M (2013). Genetics and genomics of flower initiation and development in roses. J Exp Bot. 64:847-857.
12. Causier B, Schwarz-Sommer Z and Davies B (2010). Floral organ identity: 20 years of ABCs. Semin Cell Dev Biol. 21:73-79.
13. Chakrabarty D and Datta SK (2010). Application of RAPD markers for characterization of γ-rayinduced rayinduced rose mutants and assessment of genetic diversity. Plant Biotech Rep. 4:237-242.
14. Chapuis MP and Es toup A (2006). Microsatellite null alleles and es timation of population differentiation. Mol Biol Evol. 24(3): 621-631.
15. Dakin EE and Avise JC (2004). Microsatellite null alleles in parentage analysis. Heredity. 93(5): 504-509.
16. Debener T, Janakiram T and Mattiesch L (2000). Sports and seedlings of rose varieties analysed with molecular markers. Plant Breed. 119: 71-74.
17. Debener Tand Mattiesch L (1999). Cons truction of a genetic linkage map for roses using RAPD and AFLP markers. Theor Appl Genet. 99:891-899.
18. Dubois A, Raymond O, Maene M, Baudino S, Langlade NB, Boltz V, Vergne P and Bendahmane M (2010). Tinkering with the C-function: a molecular frame for the selection of double flowers in cultivated roses. PLoS ONE, 5: e9288.
19. Farooq A, Kiani M, Khan MA, Riaz A, Khan AA, Anderson N and Byrne DH (2013).
20. Microsatellite analysis of Rosa damascena from Pakis tan and Iran. Hortic Environ Biotechnol. 54: 141-147.
21. Göktürk Baydar N, Baydar H and Debener T (2004). Analysis of genetic relationships among Rosa damascena plants grown in Turkey by using AFLP and microsatellite markers. J Biotechnol 111:263-267.
22. Gudin S (2000). Rose: genetics and breeding. Plant Breed Rev. 17:159-189.
23. Gudin S (2003). Seed propagation. In: Roberts AV, Debener T and Gudin S (Eds), Encyclopedia of Rose Science. Elsevier, pp. 620-623.
24. Hibrand-Saint Oyant L, Crespel L, Rajapakse S, Zhang L and Foucher F (2008). Genetic linkage maps of rose cons tructed with new microsatellite markers and locating QTL controlling flowering traits. Genet Genom. 4:11-23.
25. Iwata H, Tsuneo K and Ohno S (2000). Triparental origin of Damask roses. Gene. 259:53-59.
26. Jones S (2013). The inheritance of plant and flower traits in rose. BSc, Texas A&M University; USA. Karami A, Khosh-Khui M, Salehi H and Saharkhiz J (2012). Correlation between anthocyanin and essential oil content of Damask rose (Rosa damascena Mill.). J Med Pl By-Prod. 1:3-6.
27. Kiani M, Zamani Z and Khalighi A (2010). Microsatellite analysis of Iranian Damask rose (Rosa damascena Mill.) germplasm. Plant Breed. 129:551-557.
28. Kimura T, Nishitani C, Iketani H, Ban Y and Yamamoto T (2006). Development of microsatellite markers in rose. Mol Ecol Notes. 6:810-812.
29. Khai TH and Lang NT (2005). Using SSR marker to identify allele variation of somaclonal mutants in Indica rice. Omonrice 13: 121-125.
30. Li C, Zhang Y, Ying K, Liang X and Han B (2004). Sequence variation of simple sequence repeated on chromosome 4 in two subspecies of Asian cultivated rice. Theor Appl Genet, 108:392-400.
31. Nedkov N, Dobreva A, Kovacheva N, Bardarov V and Velcheva A (2009). Bulgarian rose oil of white oil-bearing rose. BJAS. 15(4):318-322.
32. Pirseyedi SM, Mardi M, Davazdahemami S, Kermani JM and Mohammadi SA (2005). Analysis of the genetic diversity between 12 Iranian Damask rose (Rosa damascena Mill.) using amplified fragment length polymorphism markers. Iran J Biotechnol. 3:225-230.
33. Raev R (1984). Mutataion breeding of Kazanlushka oil bearing rose (Rosa damascena Mill.). Ras tenievudni Naouki, 8:92-92.
34. Rusanov K, Kovacheva N, Atanassov A and Atanassov I (2005). Lessons from the microsatellite characterization of segregating population derived from seeds of open-pollinated Rosa damascena Mill. plants. Biotechnol Biotech Eq. 19:72-79.
35. Rusanov K, Kovacheva N, Atanassov A and Atanassov I (2009). Rosa damascena-genetics of a complex allotetraploid species and perspectives for molecular breeding. Biotechnol Biotech Eq. 23:594-596.
36. Rusonov K, Kovacheva N, Vosman B, Zhang L, Rajapakse S, Atanassov A and Atanassov I (2005).
37. Microsatellite analysis of Rosa damascena Mill. accessions reveals genetic similarity between genotypes used for rose oil production and old Damask rose varieties. Theor Appl Genet. 111:804-809.
38. Sarı K (2018). Determination of flower and scent characteris tics in oil-bearing rose (Rosa damascena Mill.) genotypes derived from mutation breeding. Suleyman Demirel University, Graduate School of Natural and Applied Sciences, Mas ter’s thesis, Isparta.
39. Schmitzer V, Veberic R, Os terc G and S tampar F (2010). Color and phenolic content changes during flower development in groundcover rose. J Amer Soc Horti Sci. 135 (3):195-202.
40. Tanaka Y, Sasaki N and Ohmiya A (2008). Biosynthesis of plant pigments. Plant J. 54:733-749.
41. Torres AM, Millan T and Cubero JT (1993). Identifying Rose cultivars using RAPD markers. HortSci. 28:333-334.
42. TUIK (2020). https://data.tuik.gov.tr/Kategori/ GetKategori?p=tarim-111&dil=1. Date of access: 11 February 2020.
43. Tsvetkov R (1984). Mutation Selection in Kazanlak Rose Oil. Plant Sci. 8:92-98.
44. Wang N and Shi L, (2015). Screening of mutations by TILLING in plants. Methods Mol Biol. 1245:193-203.
45. Wu KS and S teven DT (1993). Abundance, polymorphism and genetic mapping of microsatellite in rice. Mol Gen Genet. 241:256-235.
46. Yıldırım B (2016). Dis tribution of Rosa damascena Mill. in Türkiye, characterization and yield analyses in Isparta conditions” Süleyman Demirel University, Graduate School of Natural and Applied Sciences, Department of Biology, Ph.D thesis, Isparta.