Regeneration of Ruta graveolens Transgenic Plants

Yıl 2017, Cilt: 3 Sayı: 1, 66 - 71, 31.01.2017

Nadiia A. Matvieieva

Öz

Here we report the construction of transgenic Ruta graveolens L. plants. The plants were obtained via direct shoot
regeneration from leaves and petioles on Murashige and Skoog (MS) solidified medium supplemented with 6-benzylaminopurine
(1.0 mg L-1) and α-naphthylacetic acid (0,05 mg L-1) in case of A. tumefaciens-mediated transformation as
well as via direct shoot regeneration from rue “hairy” root culture on hormone free half-strength MS medium in case
of A. rhizogenes-mediated transformation. Frequency of “hairy” root formation was up to 30%. All transgenic root
lines were able to form shoots. Using of A. tumefaciens allowed to obtain transgenic rue plants with high frequency
up to 100%. Indolil butyric acid addition to the medium in concentration 0.5 mg L-1 enabled transgenic plant rooting.
The shoots regenerated from rue “hairy” root culture were more sensitive to the growth regulator addition and rooted
in 2 weeks. Rooting of the shoots obtained after A. tumefaciens-mediated transformation was observed only in 6 weeks
under these cultivation conditions.

Anahtar Kelimeler

Ruta graveolens L., A. tumefaciens, A. rhizogenes, genetic transformation, plant regeneration, “hairy” root culture

Kaynakça

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