The effects of some organic solvents on the modified Ellman procedure for the assay of cholinesterases

Year 2020, Volume: 3 Issue: 3, 153 - 158, 31.12.2020

Tugba Ercetin , Açelya Mavideniz , Karar Shukur Ali Khosravi Bahamehr Riazi Fatameh Salamati Houra Shahinfar Mohammed Najjaran Muzahem Ahmed Nasim Maher Negar Khajeh Nezhla Davdar Salva Shahinfar Hayrettin Ozan Gülcan

Abstract

Ellman’s method is the most acknowledged procedure in order to determine the cholinesterase inhibitory potential of substances. It is a quick and high-throughput screening method. Therefore, it is widely used to measure the activities of original molecules and mixtures in aqueous solvents through a UV method. Regarding that many original or known substances as well as mixtures require some organic solvent to guarantee dissolution within this methodology, it becomes critical to be aware of the inhibitory potential of organic solvents. From this perspective, within the present study, it was aimed to screen the inhibitory potential of some organic solvents on human acetylcholinesterase and human butyrylcholinesterase enzymes. The results displayed the potent inhibitory function of DMSO. On the other hand, alcohols also pointed out varying degrees of deactivation of the catalytic function of cholinesterases.

Keywords

Organic solvents , acetylcholinesterase , Butyrylcholinesterase , Ellman’s method

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