Marginal microleakage at the adhesive bonding interface submitted to storage in water, chemical aging or thermocycling

Rafael Leonardo Xediek Consani; Pedro Ivo Da Graça Fagundes; Ricardo Armini Caldas

doi:10.26650/eor.20251440402

Marginal microleakage at the adhesive bonding interface submitted to storage in water, chemical aging or thermocycling

Abstract

Purpose

The aim of this in vitro study was to evaluate the marginal microleakage of the dentin/resin interface when subjected to water storage, chemical aging, or thermocycling in different adhesive luting treatments.

Materials and Methods

Bovine teeth were used to prepare dentin blocks (5 × 4 × 1 mm) with one fully exposed dentin surface (n = 45). The blocks were randomly assigned to three luting protocols: 2BSE+DRC (two-bottle self-etch adhesive + dual-curing resin cement); SAP+PRC (self-adhesive primer + photo-activated dual resin cement); and SAP+SRC+O (self-adhesive primer + self-cured dual resin cement + Oxyguard II). Composite resin blocks of the same dimensions were luted onto the dentin blocks according to each protocol. Each treatment group (n = 15) was subdivided into three subgroups (n = 5) based on the aging challenge: storage in water for 7 days (control), aging in 10% sodium hypochlorite solution for 1 hour, or thermocycling for 5,000 cycles. Specimens were then individually immersed in a neutral methylene blue solution for 1 hour, rinsed with water, air-dried, and analyzed for marginal microleakage.

Results

Data analyzed by ANOVA and Tukey’s test (α = 0.05) showed that 2BSE+DRC exhibited the lowest microleakage after water storage for 7 days (100 µm) and the highest after thermocycling (220 µm), while sodium hypochlorite aging resulted in an intermediate value (190 µm). SAP+PRC and SAP+SRC+O showed no statistically significant differences in microleakage when stored in water (170 µm and 620 µm, respectively) or after sodium hypochlorite aging (180 µm and 610 µm, respectively). SAP+SRC+O resulted in significantly greater microleakage in water storage (620 µm) and sodium hypochlorite aging (610 µm) compared with 2BSE+DRC (100 µm and 190 µm, respectively) and SAP+PRC (170 µm and 180 µm, respectively). Thermocycling produced significant differences among the three treatments (p < 0.05), with SAP+SRC+O showing the highest microleakage (1,370 µm), 2BSE+DRC the lowest (220 µm), and SAP+PRC an intermediate value (810 µm).

Conclusion

Adhesive luting treatments exhibited different levels of marginal microleakage when subjected to water storage, chemical aging, or thermocycling. Thermocycling resulted in the highest marginal microleakage at the dentin/resin interface for all adhesive protocols tested

Keywords

References

Medić V, Obradović-Djuričić K, Dodić S, Petrović R. In vitro evaluation of microleakage of various types of dental cements. Srp Arh Celok Lek 2010;138:143-9. [CrossRef] google scholar
Piwowarczyk A, Lauer HC, Sorensen JA. Microleakage of various cementing agents for full-cast crowns. Dent Mater 2005;21:445-53. [CrossRef] google scholar
Bergenholtz G, Cox CF, Loesche WJ, Syed SA. Bacterial leakage around dental restorations: its effect on the dental pulp. J Oral Pathol Med 1982;11:439-50. [CrossRef] google scholar
About I, Murray PE, Franquin J-C, Remusat M, Smith AJ. The effect of cavity restoration variables on odontoblast cell numbers and dental repair. J Dent 2001;29:109-17. [CrossRef] google scholar
Gu X-H, Kern M. Marginal discrepancies and leakage of all-ceramic crowns: Influence of luting agents and aging conditions. Int J Prosthodont 2003;16:109-16. google scholar
Pashley DH, Tay FR, Breschi L, Tjäderhane L, Carvalho RM, Carrilho M, et al. State of the art etch-and-rinse adhesives. Dent Mater 2011;27:1-16. [CrossRef] google scholar
Takamizawa T, Barkmeier W, Tsujimoto A, Scheidel D, Erickson R, Latta M, et al. Effect of phosphoric acid pre-etching on fatigue limits of self-etching adhesives. Oper Dent 2015;40:379-95. [CrossRef] google scholar
Van Meerbeek B, Yoshihara K, Yoshida Y, Mine A, De Munch J, Van Landuyt KL. State of the art of self-etch adhesives. Dent Mater 2011;27:17-28. [CrossRef] google scholar

Frankenberger R, Krämer N, Petschelt A. Technique sensitivity of dentin bonding: effect of application mistakes on bond strength and marginal adaptation. Oper Dent 2000;25:324-30. google scholar
Goracci C, Cury AH, Cantoro A, Papacchini F, Tay FR, Ferrari M. Microtensile bond strength and interfacial properties of self-etching and self-adhesive resin cements used to lute composite onlays under different seating forces. J Adhes Dent 2006;8:327-35. google scholar
Davidson CL, Feilzer AJ. Polymerization shrinkage and polymerization shrinkage stress in polymer-based restoratives. J Dent 1997;25:435-40. [CrossRef] google scholar
Deng D, Yang H, Guo J, Chen X, Zhang W, Huang C. Effects of different artificial ageing methods on the degradation of adhesive-dentine interfaces. J Dent 2014;42:1577-85. [CrossRef] google scholar
Crim GA, Chapman KW. Reducing microleakage in Class II restorations: An in vitro study. Quintessence Int 1994;25:781-5. google scholar
Ozcan M, Barbosa SH, Melo RM, Galhano GAP, Bottino MA. Effect of surface conditioning methods on the microtensile bond strength of resin composite to composite after aging conditions. Dent Mater 2007;23:1276-82. [CrossRef] google scholar
Cenci MS, Lund RG, Pereira CL, de Carvalho RM, Demarco FF. In vivo and in vitro evaluation of Class II composite resin restorations with different matrix systems. J Adhes Dent 2006;8:127-32. google scholar
Aguiar FHB, Santos AJS, Groppo FC, Lovadino JR. Quantitative evaluation of marginal leakage of two resin composite restorations using two filling techniques. Oper Dent 2002;27:475-9. google scholar
Giorgi MCC, Hernandes NM a P, Sugii MM, Ambrosano GMB, Marchi GM, Lima D a NL, et al. Influence of an intermediary base on the microleakage of simulated class II composite resin restorations. Oper Dent 2014;39:301-7. [CrossRef] google scholar
Özel-Bektas Ö, Eren D, Herguner-Siso S, Akin GE. Effect of thermocycling on the bond strength of composite resin to bur and laser treated composite resin. Lasers Med Sci 2012;27:723-8. [CrossRef] google scholar
Amaral FLB, Colucci V, Palma-Dibb RG, Corona SAM. Assessment of in vitro methods used to promote adhesive interface degradation: A critical review. J Esthet Restor Dent 2007;19:340-53. [CrossRef] google scholar
Xie C, Han Y, Zhao XY, Wang ZY, He HM. Microtensile bond strength of one- and two-step self-etching adhesives on sclerotic dentin: The effects of thermocycling. Oper Dent 2010;35:547-55. [CrossRef] google scholar
Nikaido T, Kunzelmann K, Chen H. Evaluation of thermal cycling and mechanical loading on bond strength of a self-etching primer system to dentin. Dent Mater 2002;18:269-75. [CrossRef] google scholar
Bajabaa S, Balbaid S, Taleb M, Islam L, Elharazeen S, Alagha S. Microleakage evaluation in class V cavities restored with five different resin composites: In vitro dye leakage study. Clin Cosmet Investig Dent 2021;27: 504-11. [CrossRef] google scholar
De Munck J, Ermis RB, Koshiro K, Inoue S, Ikeda T, Sano H, et al. NaOCl degradation of a HEMA-free all-in-one adhesive bonded to enamel and dentin following two air-blowing techniques. J Dent 2007;35:74-83. [CrossRef] google scholar
Yamauti M, Hashimoto M, Sano H, Ohno H, Carvalho RM, Kaga M, et al. Degradation of resin-dentin bonds using NaOCl storage. Dent Mater 2003;19:399-405. [CrossRef] google scholar
Fathpour K, Bazazzade A, Mirmohammadi H. A comparative study of cervical composite restorations microleakage using dental universal bonding and two-step self-etch adhesive. J Contemp Dent Pract 2021;22:1035-40. [CrossRef] google scholar
Kumbuloglu O, Lassila LVJ, User A, Vallittu PK. A study of the physical and chemical properties of four resin composite luting cements. Int J Prosthodont 2004;17:357-63. google scholar
Shafiei F, Dehghani Z, Jowkar Z. The influence of the operator’s experience on the microleakage of two universal adhesives. Clin Exp Dent Res 2021;7:951-6. [CrossRef] google scholar
Giełzak F, Szczesio-Wlodarczyk A, Bociong K. Effect of storage temperature on selected strength parameters of dual-cured composite cements. J Funct Biomater 2023;14:487. [CrossRef] google scholar
Shirang F, Birang R, Ahmadpour E, Heidari Z, Memar RO, Zarei Z, Fekrazad R. Evaluation of microleakage in resin composites bonded to an er:yag laser and bur-prepared root and coronal dentin using different bonding agentes. J Lasers Med Sci 2021;12:e74. [CrossRef] google scholar
Singh S, Bhadauria US, Sharma A, Mathur RV. Comparative evaluation of microleakage with total-etch, universal (Self-etch mode), and nano adhesive systems in class V composite restorations: An in-vitro study. Cureus 2023;15:e46766. [CrossRef] google scholar

Details

Primary Language

English

Subjects

Prosthodontics

Journal Section

Research Article

Authors

Rafael Leonardo Xediek Consani ^*
0000-0003-4122-9234
Brazil

Pedro Ivo Da Graça Fagundes
0000-0001-9243-3142
Brazil

Ricardo Armini Caldas
0000-0002-5362-4744
Brazil

Publication Date

May 6, 2026

Submission Date

February 20, 2024

Acceptance Date

October 28, 2024

Published in Issue

Year 2026 Number: 1

DOI

https://doi.org/10.26650/eor.20251440402

IZ

https://izlik.org/JA59BE48LZ

Cite

RIS / Bibtex

APA

Consani, R. L. X., Ivo Da Graça Fagundes, P., & Armini Caldas, R. (2026). Marginal microleakage at the adhesive bonding interface submitted to storage in water, chemical aging or thermocycling. European Oral Research, 1, 65-70. https://doi.org/10.26650/eor.20251440402

AMA

1.Consani RLX, Ivo Da Graça Fagundes P, Armini Caldas R. Marginal microleakage at the adhesive bonding interface submitted to storage in water, chemical aging or thermocycling. EOR. 2026;(1):65-70. doi:10.26650/eor.20251440402

Chicago

Consani, Rafael Leonardo Xediek, Pedro Ivo Da Graça Fagundes, and Ricardo Armini Caldas. 2026. “Marginal Microleakage at the Adhesive Bonding Interface Submitted to Storage in Water, Chemical Aging or Thermocycling”. European Oral Research, no. 1: 65-70. https://doi.org/10.26650/eor.20251440402.

EndNote

Consani RLX, Ivo Da Graça Fagundes P, Armini Caldas R (May 1, 2026) Marginal microleakage at the adhesive bonding interface submitted to storage in water, chemical aging or thermocycling. European Oral Research 1 65–70.

IEEE

[1]R. L. X. Consani, P. Ivo Da Graça Fagundes, and R. Armini Caldas, “Marginal microleakage at the adhesive bonding interface submitted to storage in water, chemical aging or thermocycling”, EOR, no. 1, pp. 65–70, May 2026, doi: 10.26650/eor.20251440402.

ISNAD

Consani, Rafael Leonardo Xediek - Ivo Da Graça Fagundes, Pedro - Armini Caldas, Ricardo. “Marginal Microleakage at the Adhesive Bonding Interface Submitted to Storage in Water, Chemical Aging or Thermocycling”. European Oral Research. 1 (May 1, 2026): 65-70. https://doi.org/10.26650/eor.20251440402.

JAMA

1.Consani RLX, Ivo Da Graça Fagundes P, Armini Caldas R. Marginal microleakage at the adhesive bonding interface submitted to storage in water, chemical aging or thermocycling. EOR. 2026;:65–70.

MLA

Consani, Rafael Leonardo Xediek, et al. “Marginal Microleakage at the Adhesive Bonding Interface Submitted to Storage in Water, Chemical Aging or Thermocycling”. European Oral Research, no. 1, May 2026, pp. 65-70, doi:10.26650/eor.20251440402.

Vancouver

1.Rafael Leonardo Xediek Consani, Pedro Ivo Da Graça Fagundes, Ricardo Armini Caldas. Marginal microleakage at the adhesive bonding interface submitted to storage in water, chemical aging or thermocycling. EOR. 2026 May 1;(1):65-70. doi:10.26650/eor.20251440402