Research Article

Direct and transdentinal antibacterial activity of synthetic and biomimetic agents against different Streptococcus and Lactobacillus strains

Volume: 60 Number: 1 September 7, 2026

Direct and transdentinal antibacterial activity of synthetic and biomimetic agents against different Streptococcus and Lactobacillus strains

Abstract

Purpose

This study aimed to evaluate the direct and transdentinal antibacterial activity of some synthetic and biomimetic agents against different cariogenic strains.

Materials and methods

The antibacterial activity of synthetic and biomimetic agents, including chlorhexidine digluconate (CHX) (positive control), sodium hypochlorite (NaOCl), sodium fluoride (NaF), nisin, Biorepair, UltraDex, and distilled water (negative control) against different cariogenic species, including four Streptococcus strains (S); S. mutans, S. sobrinus, S. mitis, and S. sanguinis, and three Lactobacillus strains (L); L. acidophilus, L. gasseri, and L. casei were evaluated using direct and transdentinal diffusion tests. For the transdentinal test, 0.2mm human dentin discs were used, and their permeability was standardized using a modified fluid transport apparatus. Six replicates were made to determine the inhibition zone for each tested agent against each tested bacterial strain. Data were statistically analyzed using a multivariate analysis of variance using a general linear model.

Results

CHX exhibited the highest antibacterial activity against all strains. Nisin showed moderate efficacy, particularly against S. mutans. NaOCl and Biorepair showed similar effects (p=1.000), as did NaF and UltraDex, which were least effective against Streptococcus. Biorepair showed notable transdentinal activity against S. sanguinis and L. acidophilus.

Conclusion

Chlorhexidine remains the reference standard for cavity disinfection. Nisin demonstrated promising efficacy against the tested strains. Biorepair could be successfully recommended in caries management. S. mutans’ marked resistance defines it as a primary target for antimicrobial intervention. Dentin, as a biological barrier, hinders the effectiveness of the tested antibacterial agents and should be considered in treatment planning.

Keywords

References

  1. Ergücü Z, Hiller KA, Schmalz G. Influence of dentin on the effectiveness of antibacterial agents. J Endod. 2005;31:124–9. [CrossRef ] google scholar
  2. Schmalz G, Ergücü Z, Hiller KA. Effect of dentin on the antibacterial activity of dentin bonding agents. J Endod. 2004;30:352–8. [CrossRef ] google scholar
  3. Jones CG. Chlorhexidine:is it still the gold standard? Periodontol 2000. 1997;15:55–62. [CrossRef ] google scholar
  4. Lessa FC, Nogueira I, Vargas FS, Spolidorio DM, Hebling J, Garc.a-Godoy F, et al. Direct and transdentinal antibacterial activity of chlorhexidine. Am J Dent. 2010;23:255–9. -8275 google scholar
  5. Mistry KS, Sanghvi Z, Parmar G, Shah S. The antimicrobial activity of Azadirachta indica, Mimusops elengi, Tinospora cardifolia, Ocimum sanctum and 2% chlorhexidine gluconate on common endodontic pathogens:an in vitro study. Eur J Dent. 2014;8:172–7. [CrossRef ] google scholar
  6. Siqueira JF Jr, Batista MM, Fraga RC, de Uzeda M. Antibacterial effects of endodontic irrigants on black-pigmented gram-negative anaerobes and facultative bacteria. J Endod. 1998;24:414–6. [CrossRef ] google scholar
  7. Marinho VC, Higgins JP, Sheiham A, Logan S. Combinations of topical fluoride (toothpastes, mouthrinses, gels, varnishes) versus single topical fluoride for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2004;2004:CD002781. [CrossRef ] google scholar
  8. Marinho VC, Higgins JP, Sheiham A, Logan S. One topical fluoride (toothpastes, or mouthrinses, or gels, or varnishes) versus another for preventing dental caries in children and adolescents. Cochrane Database Syst Rev. 2004;2004:CD002780. [CrossRef ] google scholar

Details

Primary Language

English

Subjects

Dental Therapeutics, Pharmacology and Toxicology

Journal Section

Research Article

Publication Date

September 7, 2026

Submission Date

May 6, 2025

Acceptance Date

October 8, 2025

Published in Issue

Year 2026 Volume: 60 Number: 1

APA
Eldeeb, H., Omran, M., & Mobarak, E. (2026). Direct and transdentinal antibacterial activity of synthetic and biomimetic agents against different Streptococcus and Lactobacillus strains. European Oral Research, 60(1), 211-221. https://doi.org/10.26650/eor.20261692904
AMA
1.Eldeeb H, Omran M, Mobarak E. Direct and transdentinal antibacterial activity of synthetic and biomimetic agents against different Streptococcus and Lactobacillus strains. EOR. 2026;60(1):211-221. doi:10.26650/eor.20261692904
Chicago
Eldeeb, Heba, Maha Omran, and Enas Mobarak. 2026. “Direct and Transdentinal Antibacterial Activity of Synthetic and Biomimetic Agents Against Different Streptococcus and Lactobacillus Strains”. European Oral Research 60 (1): 211-21. https://doi.org/10.26650/eor.20261692904.
EndNote
Eldeeb H, Omran M, Mobarak E (September 1, 2026) Direct and transdentinal antibacterial activity of synthetic and biomimetic agents against different Streptococcus and Lactobacillus strains. European Oral Research 60 1 211–221.
IEEE
[1]H. Eldeeb, M. Omran, and E. Mobarak, “Direct and transdentinal antibacterial activity of synthetic and biomimetic agents against different Streptococcus and Lactobacillus strains”, EOR, vol. 60, no. 1, pp. 211–221, Sept. 2026, doi: 10.26650/eor.20261692904.
ISNAD
Eldeeb, Heba - Omran, Maha - Mobarak, Enas. “Direct and Transdentinal Antibacterial Activity of Synthetic and Biomimetic Agents Against Different Streptococcus and Lactobacillus Strains”. European Oral Research 60/1 (September 1, 2026): 211-221. https://doi.org/10.26650/eor.20261692904.
JAMA
1.Eldeeb H, Omran M, Mobarak E. Direct and transdentinal antibacterial activity of synthetic and biomimetic agents against different Streptococcus and Lactobacillus strains. EOR. 2026;60:211–221.
MLA
Eldeeb, Heba, et al. “Direct and Transdentinal Antibacterial Activity of Synthetic and Biomimetic Agents Against Different Streptococcus and Lactobacillus Strains”. European Oral Research, vol. 60, no. 1, Sept. 2026, pp. 211-2, doi:10.26650/eor.20261692904.
Vancouver
1.Heba Eldeeb, Maha Omran, Enas Mobarak. Direct and transdentinal antibacterial activity of synthetic and biomimetic agents against different Streptococcus and Lactobacillus strains. EOR. 2026 Sep. 1;60(1):211-2. doi:10.26650/eor.20261692904