The effect of Hypericum Perforatum on wound healing of oral mucosa in diabetic rats

Year 2018, Volume: 52 Issue: 3, 143 - 149, 01.09.2018

Ahmet Altan , Mutan Hamdi Aras İbrahim Damlar Hasan Gökçe Oğuzhan Özcan Cansu Alpaslan

Abstract

DOI: 10.26650/eor.2018.505

Purpose

This study aims to investigate the
histopathological and biochemical effects of the topical application of
Hypericum perforatum on the healing of surgical wounds created in the oral
mucosa of rats with experimentally induced diabetes mellitus.

Materials and methods

The study was carried out on 48 adult male
Wistar albino rats. The animals were divided into two main groups as control
and study groups. Two main groups were divided into three subgroups according
to the sacrification days. All rats were given streptozotocin 60 mg/kg, after
72 hours, and those having blood glucose levels above 200 mg/dL were included
in the study. Mucosal defects were created in the palatal area of the rats. H.
perforatum oil was applied topically twice a day to the wounds of the rats in
the study group. Animal were sacrificed on the 3rd, 7th and 10th days and
samples taken from the palatal wounds were examined histologically and
biochemically.

Results

On the 7th day, ulceration, necrosis,
epithelialization, polymorphonuclear leucocytes and hydroxyproline variables
showed statistically significant differences (p<0.05). Ulceration, necrosis
and polymorphonuclear leukocytes values were higher in the control group,
whereas epithelialization and hydroxyproline values were found to be higher in
the H. perforatum group. Among 10th-day groups, there was only a statistically
significant difference between the values of hydroxyproline, whereas H.
perforatum-treated group showed high hydroxyproline levels (p<0.05).

Conclusion

Topically applied H. perforatum did not
create any difference on the 3rd day, but it has positively affected the wound
healing on the 7th and 10th days in diabetic rats.

Keywords

Diabetes mellitus; H. perforatum; oral mucosa; St. John’s Wort; wound healing

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