Şiddetli erken çocukluk çürüğü ve erken çocukluk çürüğü bulunan hastaların dental plak ve tükürüklerinde candida varlığı: pilot çalışma

Yıl 2024, Cilt: 58 Sayı: 2, 102 - 107, 08.05.2024

Esra Özgöçmen Tuğba Yiğit Hüseyin Haydar Kutlu

https://doi.org/10.26650/eor.20241067980

Öz

Amaç: Bu çalışmanın amacı, erken çocukluk çağı çürüklerinin (ECC) ve şiddetli erken çocukluk çağı çürüklerinin (S-ECC) etyolojik faktörleri arasında yer alan Candida varlığının, çocukların dental plaklarında ve tükürüklerinde değerlendirmektir.
Gereç ve Yöntem: Çalışmamız dahil edilme kriterlerini uygun 60 katılımcı ile gerçekleştirildi. Klinik muayeneye göre her biri 20 çocuktan oluşan, S-ECC bulunan, ECC bulunan ve diş çürüğü bulunmayan üç grup oluşturuldu. Çocuklardan diş plağı ve tükürük örnekleri alındı. Alınan örnekler, laboratuvarda, Candida varlığı ve Candida türleri açısından Liofilchem® – ChromaticTM Candida (Roseto degli Abruzzi, İtalya) besiyeri kullanılarak değerlendirildi.
Bulgular: Candida varlığı, S-ECC (%40) ve ECC (%30) bulunan çocukların tükürüğünde, çürük olmayan çocuklara göre istatistiksel olarak yüksek görüldü (p<0.05). S-ECC (%25) ve ECC (%15) bulunan çocukların diş plaklarında, çürük olmayan çocuklara göre gözlemsel olarak daha fazla Candida belirlendi (p>0,05). S-ECC grubunda tükürükte Candida albicans, Candida glabrata, Candida krusei ve Candida tropikalis, diş plağında ise Candida albicans tespit edildi. ECC grubunda Candida albicans, Candida glabrata ve Candida krusei gözlenirken, çürük olmayan çocuklarda Candida görülmedi.
Sonuç: ECC patogenezinde potansiyel rol oynayan, tükürük ve dental plakta Candida varlığının bireysel risk değerlendirmesi açısından önemli olabileceği düşünülmektedir. Çalışmanın sonuçlarına göre, Candida geçiş yollarının tanımlanmasının ve önlenmesinin ECC’yi azaltmada faydalı olabileceği görülmüştür.

Anahtar Kelimeler

Candida, dental plak, diş çürüğü, tükürük

Proje Numarası

no

Presence of candida in the dental plaque and saliva of patients with severe early childhood caries and early childhood caries: a pilot study

Öz

Purpose: The aim of this study is to evaluate the presence of candida, which is one of the etiological factors contributing to early childhood caries (ECC) and severe early childhood caries (S-ECC), in the dental plaque and saliva of children aged 6 years and younger.
Materials and Methods: Our study involved 60 participants who met the inclusion criteria. Based on clinical examinations, we divided them into three groups, each consisting of 20 children: S-ECC, ECC, and caries-free groups. We collected dental plaque and saliva samples from the children during clinic visits. In the laboratory, we assessed these samples for the presence of candida using the Liofilchem® – ChromaticTM Candida (Roseto degli Abruzzi, Italy) medium and identified Candida species.
Results: The presence of Candida in the saliva of children with S-ECC (40%) and ECC (30%) was statistically significant compared to children without caries (p<0.05). Observationally, we found a higher presence of candida only in the dental plaque of children with S-ECC (25%) and ECC (15%) compared to children without caries (p>0.05). In the S-ECC group, we detected Candida albicans, Candida glabrata, Candida krusei, and Candida tropicalis in saliva, while Candida albicans was found in dental plaque. In the ECC group, Candida albicans, Candida glabrata, and Candida krusei were detected, whereas Candida was not detected in children without caries.
Conclusion: It is important to consider the presence of Candida in both saliva and dental plaque, as it potentially plays a role in the pathogenesis of ECC. These findings suggest that 3 This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record identifying and preventing Candida colonization may be valuable for individual risk assessment and could contribute to reducing ECC.

Anahtar Kelimeler

Candida, dental plaque, caries, saliva

Destekleyen Kurum

no

Proje Numarası

no

Kaynakça

• Early Childhood Caries: IAPD Bangkok Declaration. Int J Paediatr Dent 2019;29:384-6. google scholar
• Chen KJ, Gao SS, Duangthip D, Lo ECM, Chu CH. Prevalence of early childhood caries among 5-year-old children: A systematic review. J Investig Clin Dent 2019;10:e12376. google scholar
• Drury TF, Horowitz AM, Ismail AI, Maertens MP, Rozier RG, Selwitz RH. Diagnosing and reporting early childhood caries for research purposes. A report of a workshop sponsored by the National Institute of Dental and Craniofacial Research, the Health Resources and Services Administration, and the Health Care Financing Administration. J Public Health Dent 1999;59:192-7. google scholar
• Tanner AC, Mathney JM, Kent RL, Chalmers NI, Hugles CV, Loo CY, et al. Cultivable anaerobic microbiota of severe early childhood caries. J Clin Microbiol 2011;49:1464-74. google scholar
• Gupta P, Gupta N, Pawar AP, Birajdar SS, Natt AS, Singh HP. Role of sugar and sugar substitutes in dental caries: a review. ISRN Dent 2013;2013:519421. google scholar
• Thomas A, Mhambrey S, Chokshi K, Chokshi A, Jana S, Thakur S, et al. Association of oral Candida albicans with severe early childhood caries- a pilot study. J Clin Diagn Res 2016;10:109-12. google scholar
• De Carvalho FG, Silva DS, Hebling J, Spolidorio LC, Spolidorio DM. Presence of Mutans streptococci and Candida spp. in dental plaque/dentine of carious teeth and early childhood caries. Arch Oral Biol 2006;51:1024-8. google scholar
• Patel M, Shackleton JT, Coogan MM. Effect of antifungal treatment on the prevalence of yeasts in HIV-infected subjects. J Med Microbiol 2006,55:1279-84. google scholar
• Abacı Ö, Haliki A. Candida albicans’ın virulans faktörleri. Orlab On-Line Mikrobiyoloji Dergisi 2004;2:1-8. Available from: http://www.mikrobiyoloji.org/pdf/702040901.pdf google scholar
• Iwata K. Fungal toxins as a parasitic factor responsible for the establishment of fungal infections. Mycopathologia 1978;65:141-54. google scholar
• Qiu R, Li W, Lin Y, Yu D, Zhao W. Genotypic diversity and cariogenicity of Candida albicans from children with early childhood caries and caries-free children. BMC Oral Health 2015;15:144. google scholar
• Sen BH, Safavi KE, Spangberg LS. Colonization of Candida albicans on cleaned human dental hard tissues. Arch Oral Biol 1997;42:513-20. google scholar
• Cvanova M, Ruzicka F, Kukletova M, Lipovy B, Gachova D, Izakovicova Holla L, et al. Candida species and selected behavioral factors co-associated with severe early childhood caries: Case-control study. Front Cell Infect Microbiol 2022;12:943480. google scholar
• Lozano Moraga CP, Rodríguez Martínez GA, Lefimil Puente CA, Morales Bozo IC, Urzúa Orellana BR. Prevalence of Candida albicans and carriage of Candida non-albicans in the saliva of preschool children, according to their caries status. Acta Odontol Scand 2017;75:30-5. google scholar
• Beena MS, Peedikayil FC, GufranAfmed MB, Chandru TP, Soni K, Dhanesh N. Comparison of Candida species isolated from children with and without early childhood caries: A descriptive cross-sectional study. J Indian Soc Pedod Prev Dent 2017;35:296-300. google scholar
• Löe H. The gingival index, the plaque index and the retention index systems. J Periodontol 1967;38:610-6. google scholar
• Silness J, Loe H. Periodontal disease in pregnancy. II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 1964;22:121-35. google scholar
• Bulut G, Sorguç Y. Oral kandida sıklığı ve türlerinin şiddetli erken çocukluk çağı çürüğü ile ilişkisi: Mikrobiyolojik in-vivo çalışma. İzmir Dr Behçet Uz Çocuk Hast Dergisi 2020;10:219-24. google scholar
• Klinke T, Guggenheim B, Klimm W, Thurnheer T. Dental caries in rats associated with Candida albicans. Caries research 2011;45:100 -6. google scholar
• Talay T, Odabaş M. Çürük oluşumunda candida ve non-candida türlerinin etkisi. Selcuk Dental Journal 2022;9:352-8. google scholar
• Falsetta ML, Klein MI, Colonne PM, Scott-Anne K, Gregoire S, Pai CH, et al. Symbiotic relationship between Streptococcus mutans and Candida albicans synergizes virulence of plaque biofilms in vivo. Infect Immun 2014;82:1968-81. google scholar
• Hajishengallis E, Parsaei Y, Klein MI, Koo H. Advances in the microbial etiology and pathogenesis of early childhood caries. Mol Oral Microbiol 2017;32:24-34. google scholar
• O´Sullivan JM, Jenkinson HF, Cannon RD. Adhesion of Candida albicans to oral streptococci is promoted by selective adsorption of salivary proteins to the streptococcal cell surface. Microbiology 2000;146:41-8. google scholar
• Yang XQ, Zhang Q, Lu LY, Yang R, Liu Y, Zou J. Genotypic distribution of Candida albicans in dental biofilm of Chinese children associated with severe early childhood caries. Arch Oral Biol 2012;57:1048-53. google scholar
• Xiao J, Moon Y, Li L, Rustchenko E, Wakabayashi H, Zhao X, et al. Candida albicans carriage in children with severe early childhood Caries (S-ECC) and maternal relatedness. PLoS One 2016;11:e0164242. google scholar