Maturation, Fertilization and Implantation of Cattle Ova Cultured in Different Media

Yıl 2018, Sayı: 3, 181 - 188, 01.12.2018

Saif Al-hafedh Thair Mohammed İhasan Al-tamımı

Öz

Several
experiments have been conducted to study factors affecting the maturation and
fertilization of the cattle ova in the laboratory. Ovaries were collected from
77 cow reproductive systems in Al- Shuala Massacre- Baghdad. Oocytes have been
obtained from these samples by OCRC method and finishing. Oocytes were
classified into several grades from both methods, and then oocytes class A and
B only were chosen for maturation, where oocytes were incubated in multiple
media including; TCM-199, MEM, SOF, SOF+BCS, SOF+BSA in incubator 5% CO2, 90%
humidity at 38.5ºC temperature for 24 hours. Semen has been obtained by
extracting of the epididymal tail, and then collected by using a sterile 5 ml
syringe containing MEM media. Sperms were incubated with mature oocytes at a
concentration of 1 × 10 6/ml of the cultivated media in the incubator for 30
hours for the fertilization and to obtain on zygotes. Zygotes were then
isolated in the growing media. The developing embryos were monitored every 24
hours and up to 9 days until the reach blastocyst stage.  Results indicate the possibility of obtaining
embryos in vitro fertilization in an average 6.31%  (111/1604), and the best media was by via
using SOF + BCS media. The study also determined appropriate technologies to
enhance in vitro fertilization. ORCS method showed a positive effect on
increasing the rate of embryos production in vitro.

Anahtar Kelimeler

Slicing, ORCS, TCM-199, MEM, SOF, In vitro fertilization

Kaynakça

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