Laktasyonda Olan ve Laktasyonda Olmayan Kısraklarda Altrenogest Uygulaması Sonrası Serum Östrojen, Progesteron, AMH ve IGF-1 Düzeylerinin Araştırılması

Yıl 2020, Cilt: 17 Sayı: 1, 63 - 67, 23.03.2020

İbrahim Mert Polat Ömer Korkmaz Taha Burak Elifoğlu İlknur Pir Yağcı Hatice Esra Çolakoğlu Mert Pekcan

https://doi.org/10.32707/ercivet.704995

Öz

Sunulan çalışmanın amacı, altrenogest ile senkronize edilen laktasyondaki kısraklarda aşım zamanına kadar geçen süreçte ovaryumlardaki folliküler gelişimlerin ultrasonografi ile takibi, serum östrojen, progesteron, AMH ve IGF-1 konsantrasyonlarının ölçülmesi ve gebelik oranlarının değerlendirilerek bu verilerin laktasyonda olmayan kısraklarla karşılaştırılmasıdır. Çalışma, üreme sezonun başladığı ve ovaryumların aktif olduğu üreme sezonunda gerçekleştirildi; laktasyonda olan kısraklar Grup 1’e (n=8) ve laktasyonda olmayan kısraklar Grup 2’ye (n=8) dahil edildi Her iki gruba altrenogest (Regumate®) ve prostaglandin F2α ile senkronizasyon gerçekleştirildi. Laktasyonda olmayan kısrakların dominant follikül çapları laktasyonda olanlara göre daha büyük bulundu. Çalışma süresince toplanan kan örneklerinde östrojen, progesteron, AMH ve IGF-1 düzeyleri laktasyondaki kısraklarda belirgin olarak düşük bulundu. Laktasyonda olmayan kısrakların ilk aşımda gebe kalma oranı (%75), laktasyonda olan kısraklarla (%62.5) benzer bulundu (x2=1.54, P=0.62). Altronogest ile senkronize edilen kısraklarda laktasyonun ve tayın varlığının ovaryum faaliyetlerine, ovaryum steroid düzeylerine, AMH ve IGF-1 düzeylerine önemli oranda etki ettiği kanısına varıldı. Bu nedenle laktasyonda olan kısraklarda gebelik oranlarını arttırmak amacıyla altrenogest ile seksüel siklus senkronizasyonunun uygun bir yöntem olduğu düşünüldü.

Anahtar Kelimeler

Altrenogest, anti-müllerian, kısrak, laktasyon, östrojen, progesteron

The Investigation of Serum Estrogen, Progesterone, AMH and IGF-1 Profiles in Lactating and Non Lactating Mares after Altrenogest Administrations

Öz

The objective of this study is to follow up the follicular growth in ovaries until mating by ultrasonography to determine of pregnancy rates, serum estrogen, progesterone, AMH and IGF-1 concentrations in mares which synchronized with altrenogest and to compare the obtained data with non-lactating mares. The study was carried out during breeding season while ovaries were active; lactating mares were included in Group 1 (n = 8) and non-lactating mares in Group 2 (n = 8). In both groups, synchronization was performed by administration of altrenogest (Regumate®) and prostaglandin F 2 alpha (PGF2α). Dominant follicle diameters of non-lactating mares were found to be greater than those of lactating ones. During the study, estrogen, progesterone, AMH and IGF-1 levels were significantly lower in the lactating mares. The non- lactating mares were found to have a first pregnancy conception rate (75%), similar to that of lactating mares (62%) (x2=1.54, P=0.62). It was concluded that in the mares synchronized with altrenogest, the presence of lactation and the foal are significantly influences ovarian activities, ovarian steroid levels, AMH and IGF-1 levels. Therefore, in order to increase pregnancy rates in lactating mare, sexual cycle synchronization with altrenogest are considered to be a suitable method.

Anahtar Kelimeler

Altrenogest, anti müllerian, estrogen, lactation, mare, progesterone

Kaynakça

• Almeida J, Ball BA, Conley AJ, Place NJ, Liu IKM, Scholtz EL, Mathewson L, Stanley SD, Moeller BC. Biological and clinical significance of anti-Mullerian hormone determination in blood serum of the mare. Theriogenology 2011; 76(8); 1393-403.
• Ball BA, Conley AJ, MacLaughlin DT, Grundy SA, Sabuer K, Liu IKM. Expression of anti-Müllerian hormone (AMH) in equine granulosa-cell tumors and in normal equine ovaries. Theriogenology 2008; 70: 968-977.
• Block SS, Butler RW, Ehrhardt RA, Bell AW, Van Amburgh ME, Boisclair YR. Decreased concentration of plasma leptin in periparturient dairy cows is caused by negative energy balance. J Endocrinol 2001; 171: 339-48.
• Brinsko SP, Blanchard TL, Varner DD, Schumacher J, Love CC, Hinrichs K, Hartman D. Manual of Equine Reproduction. Third Edition. Mosby Elsevier, 2010; p. 124-8.
• Claes A, Ball BA, Scoggin KE, Esteller-Vico A, Kalmar JJ, Conley AJ, Squires EL, Troedsson MH. The interrelationship between anti-Müllerian hormone, ovarian follicular populations and age in mares. Equine Vet J 2014; 47: 537-41.
• Collins CW, Monfort SL, Vick MM, Wolfe BA, Weiss RB, Keefer CL, Songsasen N. Oral and injectable synthetic progestagens effectively manipulate the estrous cycle in the Przewalski's horse (Equus ferus przewalskii). Animal Reproduction Science 2014; 148(1-2): 42-52.
• Ginther OJ, Beg MA, Bergfelt DR, Kot K. Activin A, estradiol, and free insulin-like growth factor I in follicular fluid preceding the experimental assumption of follicle dominance in cattle. Biol Reprod 2002; 67: 14-9.
• Heidler B, Parvizi N, Sauerwein H, Bruckmaier RM, Heintges U, Aurich JE, Aurich C. Effects of lactation on metabolic and reproductive hormones in Lipizzaner mares. Domest Anim Endocrinol 2003; 25: 47-59.
• Hodson DJ, Townsend J, Gregory SJ, Walters C, Tortonese DJ. Role of prolactin in the gonadotroph responsiveness to gonadotrophin releasing hormone during the equine annual reproductive cycle. J Neuroendocrinol 2010; 22(6): 509-17.
• Hull KL, Harvey S. Growth hormone: a reproductive endocrine-paracrine regulator? Rev Reprod 2000; 5: 175-82.
• Izadyar F, Hage WJ, Colenbrander B, Bevers MM. The promotory effect of growth hormone on the developmental competence of in vitro–matured bovine oocytes is due to improved cytoplasmic maturation. Mol Reprod Develop 1998; 49: 444-53.
• Korosue K, Murase H, Sato F, Ishimaru M, Harada T, Watanabe G, Taya K, Nambo Y. Successful induction of lactation in a barren Thoroughbred mare: growth of a foal raised on induced lactation and the corresponding maternal hormone profiles. J Vet Med Sci 2012; 74(8): 995-1002.
• Koyla GR, Sheryt SK, Lynn GN. Follicular growth and estradiol concentrations in foaling and nonparturient mares. J Equine Vet Sci 2000; 20(4): 266-8.
• Perkins NR, Grimmett JB. Pregnancy and twinning rate in thoroughbred mares following the administration of human chorionic gonadotropine (hCG). N Z Vet J 2001; 49: 94-100.
• Salazar-Ortiz J, Monget P, Guillaume D. The influence of nutrition on the insulin-like growth factor system and the concentrations of growth hormone, glucose, insulin, gonadotropins and progesterone in ovarian follicular fluid and plasma from adult female horses (Equus caballus). Reprod Biol Endocrinol 2014; 12(1): 72.
• Satue K, Gardon KJ. A Review of the estrous cycle and the neuroendocrine mechanisms in the mare. J Steroids Horm Sci 2013; 115(4): 1-8.
• Van Niekerk CH, Van Niekerk FE. The effect of dietary protein on reproduction in the mare. VII. Embryonic development, early embryonic death, foetal losses and their relationship with serum progestagen. J S Afr Vet Med Assoc 1998a; 69: 150-5.
• Van Niekerk FE, Van Niekerk CH. The effect of dietary protein on reproduction in the mare. VI. Serum progestagen concentrations during pregnancy. J S Afr Vet Assoc 1998b; 69: 143–9.
• Vernunft A, Schneider F, Tuchscherer A, Becker F, Kanitz W. AntiMüller-Hormon (AMH) kann zur vorhersage des follikelwachstums bei stuten dienen. Pferdeheilkunde 2011; 27(3): 293-6.