Farklı Yerel Patlıcan (Solanum melongena L.) Genotiplerinin Transformasyon Etkinliklerinin Belirlenmesi

Öz

Bu çalışmada Türkiye’de yaygın olarak yetiştirilen farklı yerel patlıcan genotiplerinin transformasyon etkinliklerinin belirlenmesi amaçlanmıştır. Çalışmada bitkisel materyal olarak 7 farklı yerel patlıcan genotipi(Yamula, Kemer, Topan, Pala, Aydın Siyahı, Bilecik ve Gaziantep) kullanılmıştır. Agrobacterium aracılı transformasyon için protocol 2 farklı(P1 ve P2) kullanılmıştır. Elde edilen bulgulara göre P1 Yamula ve Gaziantep genotiplerinde rejenerasyon etkinliğı %1 olarak belirlenirken diğer genotiplerden rejenerasyon elde edilememiştir. Protokol 2’de ise transformasyon etkinliği %2-13 arasında elde edilmiştir En yüksek bitkiye dönüşüm Gaziantep(% 13), ve Yamula(%11) genotiplerinden elde edilirken en düşük bitkiye dönüşüm Aydın Siyahı genotipinden elde edilmiştir. Kültüre alınan eksplantlardan elde edilen rejenerasyon etkinliği ise %2-13 arasında gerçekleşirlen en yüksek rejenerasyon etkinliğine Gaziantep genotipi(%13) sahip olmuştur. Protokol 2’de bütün genotiplerden bitkiye dönüşüm sağlanmıştır. Çalışmada elde edilen bulgular Türkiye’de lokal olarak yetişen patlıcan genotiplerinin transformasyon etkinliklerinin protokole ve genotipe göre değiştiği bunun nedeninin ise genotiplerin sahip olduğu genetik yapının transformasyon etkinliğinde önemli etkiye sahip olmasından kaynaklanmış olabileceği düşünülmektedir.

Anahtar Kelimeler

• Patlıcan
• Agrobacterium
• Gen transferi

Determination of Transformation Efficiency of Different Local Eggplant (Solanum melongena L.) Genotypes

Öz

Abstract: The objective of this study was to ascertain the transformation efficiency of diverse local aubergine genotypes cultivated extensively in Turkey.The study utilised seven distinct local aubergine genotypes (Yamula, Kemer, Topan, Pala, Aydın Black, Bilecik and Gaziantep) as plant material.Two distinct protocols (P1 and P2) were employed for Agrobacterium-mediated transformation. The outcomes of the study indicated that regeneration efficiency was 1% for the Yamula and Gaziantep genotypes in Protocol 1, while no regeneration was observed for the other genotypes.The highest regeneration efficiency was observed for the Gaziantep (13%) and Yamula (11%) genotypes, while the lowest was observed for the Aydın Siyahı genotype. The range of regeneration efficiency observed for the cultured explants was from 2-13%, with the highest recorded efficiency seen in the Gaziantep genotype (13%).It is noteworthy that all genotypes were successfully transformed in accordance with Protocol 2. The findings of this study imply that the transformation efficiency of eggplant genotypes grown in Turkey is contingent on the protocol and genotype, suggesting that the genetic composition of these genotypes significantly impacts transformation efficiency.

Anahtar Kelimeler

• Eggplant
• Agrobacterium
• Gen transformation

Kaynakça

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