Development of an Experimental Home Bleaching Agent Containing Chitosan Nanoparticles Loaded with Gypsophila arrostii Extract

Abstract

Objectives: Peroxide alternative agents are currently being developed to provide a safe and effective tooth whitening treatment, given the potential for these agents to affect hard dental tissue. In this study, it is aimed to produce an experimental home whitening gel containing Gypsophila arrostii extract (GE) loaded chitosan nanoparticles with controlled release property. Materials and Methods: GE was prepared in two different ways using water or ethanol, and the saponin amounts of both extracts were measured using gravimetric analysis methods. GE-loaded chitosan nanoparticles (GECNPs) prepared by the ionic gelation method were investigated in terms of size, zeta potential, polydispersity index (PdI), encapsulation efficiency, and release profile. The significance level was set at p<0.05. Results: The water-based extract was preferred for encapsulation due to its statistically significantly higher percentage of saponin content (45.38 ± 3.050396%) (p=0.335). According to the characterization results, GECNPs were 651±8.34 nm in average particle size, 0.242±0.02 PdI, and 44.7±0.21 mV zeta potential. Encapsulation efficiency was determined as 97.41±3.02%. GE release from GECNP was calculated as 94.11±4.03% at 48 h, and the drug release rate increased by adding peroxides. Conclusions: The controlled release system of Gypsophila arrosti extract-loaded CNPs was successfully synthesized to develop an experimental home-bleaching gel as an alternative and promising approach.

Keywords

• Gypsophila arrosti
• saponin
• chitosan
• controlled release system
• tooth bleaching

Gypsophila arrostii Ekstresi Yüklü Kitosan Nanopartikülleri İçeren Deneysel Ev Tipi Beyazlatma Ajanının Geliştirilmesi

Abstract

Amaç: Diş sert dokusu üzerindeki potansiyel yan etkileri nedeniyle sağlıklı ve etkili diş beyazlatma tedavisi için peroksit alternatifi ajanlar geliştirilmektedir. Bu çalışmanın amacı kontrollü salım özelliğine sahip çöven otu (Gypsophila arrostii) ekstresi yüklü kitosan nanopartikülleri içeren deneysel ev tipi beyazlatma jeli oluşturmaktır. Gereç ve Yöntemler: Gypsophila arrosti ekstresi (GE) su veya etanol kullanılarak iki farklı şekilde hazırlanmıştır ve her iki ekstrenin saponin miktarı gravimetrik analiz yöntemleri kullanılarak ölçülmüştür. İyonik jelleşme yöntemi kullanılarak hazırlanan GE yüklü kitosan nanopartikülleri; partikül boyutu, zeta potansiyeli, polidispersite indeksi (PdI), enkapsülasyon etkinliği ve salım profili açısından incelenmiştir. Bulgular: Su bazlı GE, istatistiksel olarak anlamlı derecede daha yüksek saponin içeriği (%45,38 ± 3,050396) (p=0,335) nedeniyle enkapsülasyon için tercih edilmiştir. GE yüklü kitosan nanopartiküllerinin ortalama boyutu 651±8,34 nm, Pdl değeri 0,242±0,02 ve zeta potansiyeli 44,7±0,21 mV olarak ölçülmüştür. Enkapsülasyon etkinliği %97,41±3,02 olarak belirlenmiştir. GECNP'den GE salımının 48 saatte %94,11 ± 4,03'e ulaştığı ve salım hızının peroksitlerin ilavesiyle arttığı gözlemlenmiştir. Sonuç: Kontrollü salım sistemi özellikli GE yüklü CNP’lerin sentezi, gelecek vaat eden alternatif bir yaklaşım olarak deneysel bir ev tipi beyazlatma jeli geliştirmek amacı başarı ile gerçekleştirilmiştir.

Keywords

• Çöven otu
• saponin
• kitosan
• kontrollü salım sistemi
• diş beyazlatma

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