Ricinus Communis'in İnsan Diş Eti Fibroblast Hücrelerinin Canlılığı Üzerine Etkisi: Bir In Vitro Çalışma

Abstract

Amaç: Bu çalışmanın amacı, Ricinus communis ekstresinin insan diş eti fibroblastlarında (iDEF) hücre canlılığı üzerindeki potansiyel etkilerini değerlendirmektir. Gereç ve Yöntemler: Ricinus communis tohumlarından ultrasonik yöntem ve metanol çözücüsü kullanılarak ekstre elde edilmiştir. Hazırlanan ekstre 5 µg/ml, 10 µg/ml ve 15 µg/ml olacak şekilde üç farklı konsantrasyonlara seyreltildi. iDEF hücrelerine 24 saat süreyle Ricinus communis ekstreleri uygulandı. Canlılık değerlendirmesi için 3-(4,5-dimetiltiyazol-2-il)-2-5-difeniltetrazolyum bromür (MTT) analizi uygulandı. Verilerin istatistiksel analizleri SPSS paket programı kullanılarak değerlendirildi. İstatistiksel anlamlılık p<0.05 olarak kabul edildi. Bulgular: Ricinus communis’in uygulandığı tüm konsantrasyonlarda kontrol grubuna kıyasla istatistiksel olarak anlamlı fark tespit edilmedi. Sonuç: Bu bulgular, Ricinus communis ekstresinin iDEF hücrelerinde zararlı etkiler yaratmadığını, ancak hücre canlılığı üzerinde belirgin bir olumlu etki potansiyeline sahip olmadığını göstermektedir. Dolayısıyla, Ricinus communis ekstresinin yara iyileşmesi üzerindeki potansiyel etkilerini daha kapsamlı şekilde değerlendirebilmek için ek araştırmalar gerekmektedir.

Keywords

• Ricinus communis
• fibroblastlar
• hücre canlılığı

Effect of Ricinus Communis on the Viability of Human Gingival Fibroblast Cells: An In Vitro Study

Abstract

Objective: The aim of this study is to evaluate the potential effects of Ricinus communis extract on cell viability in human gingival fibroblast cells (iDEF). Materials and Methods: Ricinus communis seeds were used to prepare the extract using ultrasonic extraction and methanol solvent. The prepared extract was diluted to concentrations of 5 µg/ml, 10 µg/ml, and 15 µg/ml. iDEF cells were treated with Ricinus communis extracts for 24 hours. Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed statistically using the SPSS software, and statistical significance was set at p<0.05. Results: No statistically significant differences were observed between the Ricinus communis treated groups and the control group at all concentrations. Conclusion: These findings suggest that Ricinus communis extract did not have harmful effects on iDEF cells, but did not show a significant positive effect on cell viability. Therefore, further research is required to comprehensively assess the potential effects of Ricinus communis extract on wound healing.

Keywords

• Ricinus communis
• fibroblasts
• cell viability

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