The Effect of Baicalin on Cisplatin-Induced Oxidative Damage in the Parotid Gland

Year 2025, Volume: 9 Issue: 3, 179 - 183, 31.12.2025

Duha İbrahimoğlu , Aleyna Muhan , Şükriye Çalışkan , Şehkar Oktay , Esin Ak

Abstract

Objective: Cisplatin is one of the widely used chemotherapeutic agents; however, its therapeutic potential is significantly limited due to oxidative tissue injury. The aim of this study is to investigate the effects of baicalin on cisplatin-induced parotid gland damage .
Materials and Methods: Twenty-eight adult female rats were randomly assigned to four groups: Control, Baicalin (BA), Cisplatin (CPDD), and CPDD + BA. Dimethyl sulfoxide (DMSO), used as a vehicle, was administered daily intraperitoneally (i.p.) to the Control and CPDD groups. Cisplatin (7 mg/kg, i.p.) was given as a single dose to the CPDD and CPDD + BA groups on the first day. Baicalin (100 mg/kg, daily, i.p.) was administered to the BA and CPDD + BA groups. On day six, all rats were sacrificed, and parotid glands were collected for histological and biochemical analyses.
Results: In the CPDD group, degenerated acinar and ductal cells with cytoplasmic vacuolization, pyknotic nuclei, dilated ducts with stagnant secretion in the lumen, and vascular congestion were observed. Additionally, significantly higher total oxidant status (TOS) and oxidative stress index (OSI) and lower total antioxidant status (TAS) were detected in this group. In the CPDD+BA group, baicalin treatment significantly reversed TAS, TOS, and OSI levels in compared to the CPDD group. Also, a prominent improvement in morphological damage was observed in the parotid gland of this group compared to the CPDD group.
Conclusion: Our findings suggest that baicalin may have a protective effect against cisplatin-induced toxicity due to its antioxidant properties

Keywords

baicalin , Cisplatin , Parotid gland , Oxidative damage

Ethical Statement

This experimental protocol was approved by the Marmara University Animal Care and Use Committee (protocol number: 02.2025.mar.1, date: 17.02.2025).

Supporting Institution

Marmara University Scientific Research Projects Commission

Project Number

TLY-2025-11886

Thanks

This study was supported by Marmara University Scientific Research Projects Commission (Project number: TLY-2025-11886).

Baikalinin Parotis Bezinde Sisplatin ile İndüklenen Oksidatif Hasar Üzerine Etkisi

Abstract

Amaç: Sisplatin, yaygın olarak kullanılan bir kemoterapötik ajan olmakla birlikte, oksidatif doku hasarı gibi yan etkileri nedeniyle terapötik potansiyeli önemli ölçüde sınırlanmaktadır. Bu çalışmanın amacı, baikalinin sisplatin kaynaklı parotis bezi hasarına karşı etkilerini araştırmaktır.
Materyal ve Yöntemler: Yirmi sekiz yetişkin dişi sıçan rastgele dört gruba ayrıldı: Kontrol, Baikalin (BA), Sisplatin (CPDD) ve CPDD + BA. Kontrol ve CPDD gruplarına, taşıyıcı solüsyon olan dimetil sülfoksit (DMSO) günlük olarak intraperitoneal (i.p.) yolla verildi. Sisplatin (7 mg/kg, i.p.) deneyin ilk gününde tek doz olarak CPDD ve CPDD + BA gruplarına uygulandı. Baikalin (100 mg/kg, günlük, i.p.) ise BA ve CPDD + BA gruplarına verildi. Altıncı günde tüm sıçanlar sakrifiye edilerek parotis bezleri histolojik ve biyokimyasal analizler için toplandı.
Bulgular: CPDD grubunda, sitoplazmik vakuolizasyon ve piknotik çekirdekler içeren hasarlı asiner ve kanal hücreleri, lümeninde salgı içeren genişlemiş kanallar ve vasküler konjesyon gözlendi. Ayrıca, bu grupta total oksidan seviyesi (TOS) ve oksidatif stres indeksi (OSİ) seviyeleri anlamlı derecede yüksek, total antioksidan seviyesi (TAS) anlamlı derecede azalmış olarak tespit edildi. CPDD+BA grubunda ise, baikalin tedavisi TAS, TOS ve OSİ seviyelerini CPDD grubuna kıyasla anlamlı şekilde tersine çevirdi. Ayrıca, CPDD grubuna kıyasla bu grubun parotis bezinde morfolojik hasarda belirgin bir iyileşme gözlenmiştir.
Sonuç: Bulgularımız, baikalinin antioksidan özellikleri nedeniyle sisplatin kaynaklı toksisiteye karşı koruyucu bir etkiye sahip olabileceğini düşündürmektedir.

Keywords

Baikalin , Sisplatin , Parotis bez , Oksidatif hasar

Project Number

TLY-2025-11886

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