Klorheksidinin İnsan Dişeti Fibroblastları Üzerindeki Hücre Canlılığı ve Sitotoksisite Etkinliğinin İn vitro Koşullarda Değerlendirilmesi

Abstract

Amaç: Bu in vitro çalışmada, % 0,2’lik klorheksidin (CHX) solüsyonunun, insan dişeti fibroblastları (HGF) üzerindeki hücre canlılığı ve sitotoksisite etkinliklerinin değerlendirilmesi amaçlanmıştır. Gereç ve Yöntemler: 30 saniye ve 2 dakikalık zaman aralıklarında, nötr pH değerindeki % 0,2’lik CHX solüsyonu kullanılarak çalışılmıştır. Hücre canlılığı ve sitotoksisitesi, HGF’ye karşı 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolyum bromür (MTT) testi ve laktat dehidrogenaz (LDH) testi kullanılarak değerlendirilmiştir. Sonuçlar ortalama ve standart sapma değerleri kullanılarak two way ANOVA testiyle istatistiksel olarak değerlendirilmiştir (p<0,05). Bulgular: MTT testi sonuçlarına göre, % 0,2 CHX solüsyonunun zamanla hücre canlılığını azalttığı gözlendi. LDH testi sonuçlarına göre ise % 0,2 CHX’in sitotoksik etkinliğinin kontrol grubuna kıyasla arttığı ancak zaman içinde istatistiksel olarak anlamlı bir fark göstermediği gözlendi. Sonuç: Bu in vitro çalışmanın sınırları dahilinde, % 0,2 CHX solüsyonu, 30 sn ve 2 dk’lık kısa maruz kalma sürelerinde, HGF hücre canlılığını azalttı ve HGF üzerine sitotoksik etkinlik gösterdi.

Keywords

• klorheksidin
• fibroblast
• in vitro
• sitotoksisite
• hücre canlılık testleri

In vitro Evaluation of Cell Viability and Cytotoxicity Effects of Chlorhexidine on Human Gingival Fibroblasts

Abstract

Objectives: The aim of this study was to evaluate the cell viability and cytotoxicity effects of a 0.2 % chlorhexidine (CHX) solution on human gingival fibroblasts (HGF) in vitro. Materials and Methods: A 0.2 % CHX solution with a neutral pH was tested at 30 second and 2 minute intervals. Cell viability and cytotoxicity against HGF were determined using the 3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test and the lactate dehydrogenase (LDH) test. Results were evaluated by calculating means and standard deviations using the two-way ANOVA test (p<0.05). Results: According to the MTT test results, it was observed that a 0.2 % CHX solution decreased cell viability over time. According to the LDH test results, the cytotoxic efficacy of 0.2 % CHX was higher compared to the control group, but did not show a statistically significant difference over time. Conclusions: Within the limits of this in vitro study, 0.2 % CHX solution reduced HGF cell viability and showed cytotoxic activity on HGF during short exposure times of 30 sec and 2 min.

Keywords

• Chlorhexidine
• fibroblasts
• in vitro
• cytotoxicity
• cell viability assays

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