Erzincan University Journal of Science and Technology

2149-4584

Erzincan Binali Yıldırım Üniversitesi

10.18185/erzifbed.1533306

Pharmaceutical Sciences

Eczacılık Bilimleri

Optimization of protein extraction from mallow leaves: enhancing yield through various extraction methods

https://orcid.org/0000-0001-6608-7231

Aksoy Caf

Zülal

EGE UNIVERSITY, FACULTY OF ENGINEERING, DEPARTMENT OF FOOD ENGINEERING, FOOD ENGINEERING PR.

https://orcid.org/0000-0001-9862-0176

Akyüz

Ayça

EGE ÜNİVERSİTESİ, MÜHENDİSLİK FAKÜLTESİ, GIDA MÜHENDİSLİĞİ BÖLÜMÜ, GIDA MÜHENDİSLİĞİ PR.

https://orcid.org/0000-0003-0475-4099

Ersus

Seda

EGE UNIVERSITY, FACULTY OF ENGINEERING, DEPARTMENT OF FOOD ENGINEERING, FOOD ENGINEERING PR.

12 31 2024

SUIC 25 36 08 15 2024 12 13 2024

2008

Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi

This study focuses on mallow leaves as a potential protein source, aiming to enhance protein yield through various extraction methods. Firstly, frozen, vacuum-dried, and freeze-dried mallow leaves were compared in terms of protein content and the freezing (42.33%) has the highest protein content in dry basis. In order to increase protein yield, as the second step, the extraction process was carried out at different pH values (9 and 10) and the highest protein concentration of 1.535 mg/mL was detected at pH 10. Step 3 was the extraction temperature, which is performed at 55, 60 and 65 ̊C and the protein content of protein concentrates were 50.07%, 45.31% and 36.96%, respectively. In the next step, 10, 15 and 20 mL/g solvent/solid ratios were tried, and 10 mL/g had the highest protein content (53.07%). As the last step, the optimum ultrasound-assisted extraction parameters were determined as 20 min at 100% amplitude. Finally, the protein yield reached 60% with all these process steps.

Mallow leaf Plant protein Optimization Ultrasound assisted extraction

European Union's Horizon 2020-PRIMA Section I Program, Exploration and Implementation of Products with Alternative Proteins in the Mediterranean Region (ProxIMed)

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