Erzincan University Journal of Science and Technology 2149-4584 Erzincan Binali Yıldırım Üniversitesi 10.18185/erzifbed.880524 Engineering Mühendislik Yaygın İnorganik Anyonların Çay Örneklerinde İyon Kromatografik Tayini Ion Chromatographic Determination of Common Inorganic Anions in Tea Samples https://orcid.org/0000-0003-0377-5656 Cittan Mustafa Manisa Celal Bayar University 03 31 2021 14 1 168 174 02 15 2021 03 01 2021 Copyright © 2008, Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi 2008 Erzincan Üniversitesi Fen Bilimleri Enstitüsü Dergisi

Bu çalışmada, poşet çaylarda bulunan inorganik anyonları - florür, klorür, nitrit, bromür, nitrat, fosfat ve sülfat - belirlemek için, iletkenlik detektörü ile eşleştirilmiş iyon kromatografisine dayalı basit, hızlı ve doğru bir teknik tanımlanmıştır. Analitlerin kromatografik ayrılması, Dionex IonPac AG9-HC 4×50 mm ön kolonu ile birleştirilmiş, Dionex IonPac AS9-HC 4x250 mm analitik kolon kullanılarak gerçekleştirilmiştir. Elüent olarak 12 mM KOH çözeltisi kullanılmıştır. İnorganik anyonlar için tayin limitleri, 0.02 - 0.20 mg L-1 aralığında belirlenmiştir. Çay infüzyonları geleneksel olarak poşet çayların 200 mL hacimli kaynar ultra saf su ile demlenmesi yoluyla hazırlanmıştır. Söz konusu anyonların ekstraksiyonuna demleme süresinin etkisi incelenmiş ve elde edilen sonuçlara göre optimum demleme süresi 6 dakika olarak belirlenmiştir. Son olarak tarif edilen kromatografik yöntem bazı siyah, yeşil ve beyaz poşet çay örneklerindeki inorganik anyonların analizleri için uygulanmış ve beş adet anyon numunelerde nicel olarak belirlenmiştir. Tüm örneklerdeki nitrit ve bromür miktarları ise yöntemin tayin limitlerinin oldukça altında tespit edilmiştir.

A simple, rapid, and accurate technique based on ion chromatography coupled with a suppressed conductivity detector was described to determine the inorganic anions – fluoride, chloride, nitrite, bromide, nitrate, phosphate, and sulphate in tea bag samples. A Dionex IonPac AS9-HC 4×250 mm analytical column with a Dionex IonPac AG9-HC 4×50 mm guard column was used for the chromatographic separation of the analytes. The eluent was 12 mM KOH and the limit of quantification for the inorganic anions were in the range 0.02 - 0.20 mg L-1. Tea infusions were prepared traditionally by pouring the tea bags with 200 mL of boiling ultra-pure water. Influence of the brewing time on the extraction of inorganic anions from the tea bags was examined and optimum brewing time was determined as 6 min according to the results obtained. Finally, the ion chromatographic technique was applied to analyze the inorganic anions in some black, green, and white tea bag samples and five anions were determined quantitatively. The nitrite and bromide amount in all the samples were well below the quantification limit of the method.

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