DEVELOPMENT AND CHARACTERIZATION OF VITAMIN B9 - ELECTROSPRAYED NON-WOVEN SURFACES FOR WOUND HEALING APPLICATIONS
Abstract
In this study, novel wound dressing materials based on non-woven (NW) surfaces were developed using electrospraying (e-spraying) process. Polyester spunbond (PET SPB), polypropylene spunbond (PP SPB), and polypropylene melt-blown (PP MB) surfaces used as matrix, and folic acid (FA), vitamin B9 were sprayed on these surfaces. The resulting NW fabrics with the same FA content were investigated in terms of chemical, morphological, thermal, wettability properties. Scanning Electron Microscopy and (SEM) and Fourier Transform Infrared (FT-IR) spectroscopy results showed formation of physical interaction between NWs and FA is successfully deposited onto NWs with average fiber diameters from 2.6 µm to 23.11 µm. According to the thermogravimetric analysis (TGA) FA loaded-PP SPB have enhaced thermal stability compared to pure one (PP SPB). The FA-loaded surfaces have hydrophobic property with contact angles values more than >90°. The in-vitro release was carried out by UV-Vis within the 8 hour-period phosphate buffer saline (pH 7.2). The results indicated that FA-loaded surfaces have a fast release behaviour. The total FA release amounts of the FA-loaded PET SBP, PP SPB and PP MB NWs were found as 22.8, 17.1, and 17.5 ppm. Moreover, biocompatibility of all resulting NW surfaces was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and neutral red uptake (NRU) cytotoxicity tests in L929 cell lines. The obtained NWs have biocompatible and non-toxic material except PET SPB-sFA.The study indicated that FA-loaded NWs can be potential candidates for wound healing applications.
Keywords
Non-woven, wound healing, folic acid, characterization, in vitro study
Supporting Institution
Bursa Technical University
Project Number
190D003
Thanks
This study was supported by Bursa Technical University Scientific Research Projects Coordination Unit (SRP Project No. 190D003).
The authors would like to thank Mogul Textile Company or their kind donation of spunbond and melt-blown non-woven fabrics and Bursa Technical University Central Research Laboratory (Bursa, Turkey) for the SEM and TGA analysis. The authors would like to thank Cantekin Kaykılarlı and Metallurgical and Materials Engineering Department for pycnometer test.
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