DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING

Ceren Özel; Tayfun Şengel; Aliakbar Ebrahimi; Elif Apaydın; Hamed Ghorbanpoor; Ayla Eker Sarıboyacı; Onur Uysal; Mete Ozkurt; Hüseyin Avcı

doi:10.18038/estubtda.984324

EN

DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING

Abstract

The currently used approaches in the treatment of wounds and burns have been studied for many years to eliminate problems related with mechanical strength, elasticity, biocompatibility and cost. Nowadays, fabrication of composite fibers by a fiber as core and hydrogels as shell, which can be seeded by cells is rapidly increasing. In this study, it is aimed to produce a natural polymer-based dressing that can provide controlled antibiotic release to accelerate wound healing with low cost and high efficiency. The composites have been achieved by using surgical suture as a core and alginate in the shell part, which modified with starch and gelatin. Evaluating low-cost hydrogel material such as alginate, starch and gelatin in the shell layer of composite fibers by different concentrations were investigated in addition to study their swelling and drug release behaviors. The parameters for the model of an antibiotic release that can prevent common infections can be manipulated by using a biotextile-based approach to quantify the amount of antibiotics and its release to satisfy clinical requirements. Toluidine blue and Penicillin/Streptomycin were chosen as antibiotic models for drug release experiments. Moreover, human adipose tissue-derived mesenchymal stem cells (hAT-MSCs) were applied to evaluate cell viability experiments. Results demonstrated that alginate modified starch and gelatin can be used as low-cost and promising materials for use in biomedical applications.

Keywords

Wound healing, Composite fibers, Hydrogel, Bio textile, Core-shell

Supporting Institution

TÜBİTAK

Project Number

219S647

Thanks

This study is supported by Turkish Scientific and Technological Council (TÜBİTAK-1002) under the grant number of 219S647.

References

[1] Martin P. Wound Healing--Aiming for Perfect Skin Regeneration. Science, 1997; 276: 75-81.
[2] Gonzalez ACDO, Costa TF, Andrade ZDA & Medrado, ARAP. Wound Healing-A Literature Review. Anais Brasileiros de Dermatologia 2016; 91:614-620.
[3] Tatara AM, Kontoyiannis DP, Mikos AG. Drug Delivery and Tissue Engineering to Promote Wound Healing in the Immunocompromised Host: Current Challenges and Future Directions. Advanced Drug Delivery Reviews, 2018; 129: 319-329.
[4] Costa‐Almeida R, Domingues RM, Fallahi A, Avci H, Yazdi IK, Akbari M, et al. Cell‐Laden Composite Suture Threads for Repairing Damaged Tendons. Journal of tissue Engineering and Regenerative Medicine, 2018; 12:1039-1048.
[5] Fallahi A, Yazdi IK, Serex L, Lesha E, Faramarzi N, Tarlan F, et al. Customizable Composite Fibers for Engineering Skeletal Muscle Models. ACS Biomaterials Science & Engineering 2019; 6:1112-1123.
[6] Tamayol A, Yazdi I, Fallahi A, Nabavinia M, Avci H, Costa-Almeida R, et al. Textile Tissue Engineering: a Path Towards Organ Weaving. in Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress FBIOE, 2016 doi: 10.3389/conf..
[7] Costa-Almeida R, Tamayol A, Yazdi I, Avci H, Fallahi A, Annabi N. et al. A Textile Platform using Mechanically Reinforced Hydrogel Fibres Towards Engineering Tendon Niche, European Cells and Materials, 2016; 31: 1473-2262.
[8] Adeli H, Khorasani MT and Parvazinia MJI. Wound Dressing Based on Electrospun PVA/Chitosan/Starch Nanofibrous Mats: Fabrication, Antibacterial and Cytocompatibility Evaluation and in vitro Healing Assay. International Journal of Biological Macromolecules, 2019; 122: 238-254.
[9] Baghaie S, Khorasani MT, Zarrabi A and Moshtaghian JJJ. Wound Healing Properties of PVA/Starch/Chitosan Hydrogel Membranes with Nano Zinc Oxide as antibacterial Wound Dressing Material. Polymer Edition, 2017; 28:2220-2241.
[10] Zhang M & Zhao X. Alginate Hydrogel Dressings for Advanced Wound Management. International Journal of Biological Macromolecules, 2020; 162: 1414-1428

[11] Mayet N, Choonara YE, Kumar P, Tomar LK, Tyagi C, Toit L. C. Du, et al. A comprehensive review of advanced biopolymeric wound healing systems. Journal of Pharmaceutical Sciences 2014; 103: 2211-2230.
[12] Lee KY, Mooney DJ. Alginate: Properties and Biomedical Applications. Progress in Polymer Science, 2012;37: 106-126.
[13] Gaspar-Pintiliescu A, Stanciuc AM and Craciunescu O. Natural Composite Dressings Based on Collagen, Gelatin and Plant Bioactive Compounds for Wound Healing: A Review. International Journal of Biological Macromolecules, 2019;138: 854-865.
[14] Hasnain MS, Nayak AK. Alginates: Versatile Polymers in Biomedical Applications and Therapeutics. CRC Press, 2019.
[15] Sudha PN. Industrial Applications Of Marine Biopolymers. CRC Press, 2017.
[16] Mir M, Ali MN, Barakullah A, Gulzar A, Arshad M, Fatima S, et al. Synthetic Polymeric Biomaterials for Wound Healing: A Review. Progress in Biomaterials, 2018; 7:1-21.
[17] Christensen BE. Alginates as Biomaterials in Tissue Engineering. Carbohydrate Chemistry: Chemical and Biological Approaches, 2011; 37:227-258.
[18] Torres FG, Commeaux S & Troncoso OP. Starch‐Based Biomaterials for Wound‐Dressing Applications. Starch‐Stärke, 2013; 65: 543-551.
[19] Naseri-Nosar M, Ziora ZM. Wound Dressings from Naturally-Occurring Polymers: A Review On Homopolysaccharide-Based Composites. Carbohydrate Polymers, 2018; 189:379-398.
[20] Campbell FC. Structural Composite Materials. ASM international, 2010.
[21] Malafaya PB, Silva GA & Reis RL. Natural–Origin Polymers as Carriers and Scaffolds for Biomolecules and Cell Delivery in Tissue Engineering Applications. Advanced Drug Delivery Reviews, 2007; 59: 207-233.
[22] Pourjavadi A, Ebrahimi AA & Barzegar, S. Preparation and Evaluation of Bioactive and Compatible Starch Based Superabsorbent for Oral Drug Delivery Systems. Journal of Drug Delivery Science and Technology, 2013; 23:511-517.
[23] Zhang LM, Yang C & Yan L. Perspectives on: Strategies to Fabricate Starch-Based Hydrogels with Potential Biomedical Applications. Journal of Bioactive and Compatible Polymers, 2005; 20: 297-314.
[24] Afewerki S, Sheikhi A, Kannan S, Ahadian S & Khademhosseini A. Gelatin‐Polysaccharide Composite Scaffolds for 3D Cell Culture and Tissue Engineering: Towards Natural Therapeutics. Bioengineering & Translational Medicine, 2019; 4: 96-115.
[25] Choi YS, Hong S R, Lee YM, Song KW, Park MH & Nam YS. Study on Gelatin-Containing Artificial Skin: I. Preparation and Characteristics of Novel Gelatin-Alginate Sponge. Biomaterials, 1999; 20: 409-417.
[26] Princely S, Saleem Basha N, Nandhakumar S and Dhanaraju MJSRL. Design and Evaluation of Controlled Release Gentamycin Incorporated Gelatine Alginate Matrices for Wound Management. Scholars Research Library, 2015; 7:145-153.
[27] Abdullah MF, Nuge T, Andriyana A, Ang BC and Muhamad, F. Core–Shell Fibers: Design, Roles, and Controllable Release Strategies in Tissue Engineering and Drug Delivery. Polymers, 2019; 11:2008
[28] Avci H, Ghorbanpoor H and Nurbas M. Preparation of Origanum Minutiflorum Oil-Loaded Core–Shell Structured Chitosan Nanofibers with Tunable Properties. Polymer Bulletin, 2018; 75:4129-4144.
[29] Karaöz E, Demircan PÇ, Erman G, Güngörürler E and Sarıboyacı AE. Comparative Analyses of Immunosuppressive Characteristics of Bone-Marrow, Wharton’s Jelly, and Adipose Tissue-Derived Human Mesenchymal Stem Cells. Turkish Journal of Hematology, 2017; 34:213.
[30] Özdemir AT, Özdemir RBÖ, Kırmaz C, Sarıboyacı AE, Halbutoğlları ZSÜ, Özel C, et al. The Paracrine Immunomodulatory Interactions Between The Human Dental Pulp Derived Mesenchymal Stem Cells and CD4 T Cell Subsets. Cellular Immunology, 2016; 310:108-115.
[31] Özdemir RBÖ, Özdemir AT, Sarıboyacı AE, Uysal O, Tuğlu Mİ and Kırmaz C. The Investigation of Immunomodulatory Effects of Adipose Tissue Mesenchymal Stem Cell Educated Macrophages on The CD4 T Cells. Immunobiology, 2019; 224:585-594.
[32] Zhuang Y, Yu F, Chen H, Zheng J, Ma J and Chen J. Alginate/Graphene Double-Network Nanocomposite Hydrogel Beads with Low-Swelling, Enhanced Mechanical Properties and Enhanced Adsorption Capacity. Journal of Materials Chemistry A, 2016; 4:10885-10892.
[33] Matyash M, Despang F, Ikonomidou C and Gelinsky M. Swelling and Mechanical Properties of Alginate hydrogels with Respect to Promotion of Neural Growth. Tissue Engineering Part C: Methods 2014; 20:401-411.
[34] Bajpai S and Kirar N. Swelling and Drug Release Behavior of Calcium Alginate/Poly (Sodium Acrylate) Hydrogel Beads. Designed Monomers and Polymers, 2016; 19:89-98.
[35] Roy A, Bajpai J and Bajpai A. Dynamics of Controlled Release of Chlorpyrifos from Swelling and Eroding Biopolymeric Microspheres of Calcium Alginate and Starch. Carbohydrate Polymers, 2009; 76:222-231.
[36] Yao R, Zhang R, Luan J and Lin F. Alginate and Alginate/Gelatin Microspheres for Human Adipose-Derived Stem Cell Encapsulation and Differentiation. Biofabrication, 2012; 4:025007.
[37] Pulat M. The Preparatıon of Gelatin Coated Sodium Alginate Hydrogels. The Eurasia Proceedings of Science Technology Engineering and Mathematics, 2018; 4:149-155
[38] El-Sherbiny I, Lins R, Abdel-Bary E and Harding D. Preparation, Characterization, Swelling and in vitro Drug Release Behaviour of Poly [N-acryloylglycine-Chitosan] Interpolymeric pH and Thermally-Responsive Hydrogels. European Polymer Journal, 2005; 41:2584-2591.
[39] Wang Q, Hu X, Du Y and Kennedy JF. Alginate/Starch Blend Fibers and Their Properties for Drug Controlled Release. Carbohydrate Polymers, 2010; 82:842-847.
[40] Dong Z,Wang Q and Du Y. Alginate/Gelatin Blend Films and Their Properties for Drug Controlled Release. Journal of Membrane Science, 2006; 280:37-44.
[41] Fan L, Du Y, Huang R, Wang Q, Wang X and Zhang L. Preparation and Characterization of Alginate/Gelatin Blend Fibers. Journal of Applied Polymer Science, 2005; 96:1625-1629.
[42] Rosellini E, Cristallini C, Barbani N, Vozzi G and Giusti P. Preparation and Characterization of Alginate/Gelatin Blend Films for Cardiac Tissue Engineering. Journal of Biomedical Materials Research Part A: An Official Journal of The Society for Biomaterials, The Japanese Society for Biomaterials and The Australian Society for Biomaterials and the Korean Society for Biomaterials, 2009; 91:447-453.
[43] Akbari M, Tamayol A, Laforte V, Annabi N, Najafabadi AH, Khademhosseini A. et al. Composite Living Fibers for Creating Tissue Constructs using Textile Techniques. Advanced Functional Materials, 2014; 24:4060-4067
[44] He Y, Yang F, Zhao H, Gao Q, Xia B and Fu J. Research on The Printability of Hydrogels in 3D bioprinting. Scientific Reports, 2016; 6:1-13.
[45] Axpe E and Oyen ML. Applications of Alginate-Based Bioinks in 3D Bioprinting. International Journal of Molecular Sciences, 2016; 17:1976
[46] Wells RG. The Role of Matrix Stiffness in Regulating Cell Behavior. Hepatology, 2008; 47:1394-1400.
[47] Kalia S. Polymeric Hydrogels As Smart Biomaterials. Springer, 2016; Berlin, Germany.
[48] Wang XF, Lu PJ, Song Y, Sun YC, Wang YG and Wang Y. Nano Hydroxyapatite Particles Promote Osteogenesis in A Three-Dimensional Bio-Printing Construct Consisting of Alginate/Gelatin/hASCs. RSC Advances, 2016; 6:6832-6842.

Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Ceren Özel
0000-0002-5648-3174
Türkiye

Tayfun Şengel
0000-0002-1162-6979
Türkiye

Aliakbar Ebrahimi
0000-0001-6437-7796
Türkiye

Elif Apaydın
0000-0002-2544-4068
Türkiye

Hamed Ghorbanpoor
0000-0002-2665-8172
Türkiye

Ayla Eker Sarıboyacı
0000-0003-4536-9859
Türkiye

Onur Uysal
0000-0001-6800-5607
Türkiye

Mete Ozkurt
0000-0003-4000-2345
Türkiye

Hüseyin Avcı ^*
0000-0002-2475-1963
Türkiye

Publication Date

November 30, 2021

Submission Date

August 20, 2021

Acceptance Date

November 1, 2021

Published in Issue

Year 2021 Volume: 22 Number: Vol:22- 8th ULPAS - Special Issue 2021

DOI

https://doi.org/10.18038/estubtda.984324

IZ

https://izlik.org/JA56NY52ZE

APA

Özel, C., Şengel, T., Ebrahimi, A., Apaydın, E., Ghorbanpoor, H., Eker Sarıboyacı, A., Uysal, O., Ozkurt, M., & Avcı, H. (2021). DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING. Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering, 22(Vol:22- 8th ULPAS - Special Issue 2021), 98-111. https://doi.org/10.18038/estubtda.984324

AMA

1.Özel C, Şengel T, Ebrahimi A, et al. DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING. Estuscience - Se. 2021;22(Vol:22- 8th ULPAS - Special Issue 2021):98-111. doi:10.18038/estubtda.984324

Chicago

Özel, Ceren, Tayfun Şengel, Aliakbar Ebrahimi, et al. 2021. “DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING”. Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering 22 (Vol:22- 8th ULPAS - Special Issue 2021): 98-111. https://doi.org/10.18038/estubtda.984324.

EndNote

Özel C, Şengel T, Ebrahimi A, Apaydın E, Ghorbanpoor H, Eker Sarıboyacı A, Uysal O, Ozkurt M, Avcı H (November 1, 2021) DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING. Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering 22 Vol:22- 8th ULPAS - Special Issue 2021 98–111.

IEEE

[1]C. Özel et al., “DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING”, Estuscience - Se, vol. 22, no. 22-8, pp. 98–111, Nov. 2021, doi: 10.18038/estubtda.984324.

ISNAD

Özel, Ceren - Şengel, Tayfun - Ebrahimi, Aliakbar - Apaydın, Elif - Ghorbanpoor, Hamed - Eker Sarıboyacı, Ayla - Uysal, Onur - Ozkurt, Mete - Avcı, Hüseyin. “DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING”. Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering 22/22-8 (November 1, 2021): 98-111. https://doi.org/10.18038/estubtda.984324.

JAMA

1.Özel C, Şengel T, Ebrahimi A, Apaydın E, Ghorbanpoor H, Eker Sarıboyacı A, Uysal O, Ozkurt M, Avcı H. DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING. Estuscience - Se. 2021;22:98–111.

MLA

Özel, Ceren, et al. “DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING”. Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering, vol. 22, no. Vol:22- 8th ULPAS - Special Issue 2021, Nov. 2021, pp. 98-111, doi:10.18038/estubtda.984324.

Vancouver

1.Ceren Özel, Tayfun Şengel, Aliakbar Ebrahimi, Elif Apaydın, Hamed Ghorbanpoor, Ayla Eker Sarıboyacı, Onur Uysal, Mete Ozkurt, Hüseyin Avcı. DESIGN OF ALGINATE BASED BLENDS FOR LIVING COMPOSITE FIBERS TO PROMOTE WOUND HEALING. Estuscience - Se. 2021 Nov. 1;22(Vol:22- 8th ULPAS - Special Issue 2021):98-111. doi:10.18038/estubtda.984324

Cited By

Chitosan‐Grafted Polycaprolactone Nanofibers for Inhibiting Staphylococcus epidermidis Biofilm Formation

ChemistrySelect

https://doi.org/10.1002/slct.202502074