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HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES

Yıl 2021, Cilt 22, Sayı 4, 344 - 352, 29.12.2021
https://doi.org/10.18038/estubtda.806222

Öz

Heat shock proteins are produced when the organisms are exposed to various environmental stress conditions such as temperature, light, toxins. It is a known fact that in bacteria, which has the HSP gene, antibiotics can trigger the expression of the heat shock protein. However, the response of heat shock protein genes to antibiotics has not been fully clarified in the literature yet, studies are still ongoing. In this study, a novel way for the detection heat shock protein65 was investigated using the electrochemical impedance spectroscopy due to its sensitivity, selectivity, low cost. To do so, heat shock protein65 probe and target were designed and the hybridization behavior of the probe with designed target was studied upon the binding onto screen-printed electrodes. Cyclic voltammetry was performed to analyze surface characterization of secreen printed electrodes and the performance of the screen printed electrodes was tested using electrocehimcal impedance spectroscopy by measuring changes in the charge transfer resistance upon hybridization. Based on obtained results, the designed heat shock protein65 probe was confirmed and an appropriate increase in charge transfer resistance values compliance to the literature proved that the electrochemical impedance spectroscopy can be effectively used to detect heast shock protein65 probe label-free. Results presented here can lead to development of antibiotic susceptibility assay based on the heat shock protein genes in future.

Kaynakça

  • Garred P, Brygge K, Sorensen CH, Madsen HO, Thiel S, Svejgaard A. Mycobacteria in water. J Appl Bacteriol 1984; 57: 193–211.
  • Beere HM. “The stress of dying”: The role of heat shock proteins in the regulation of apoptosis. J Cell Sci 2004; 117: 2641–2651.
  • Nguyen L. Antibiotic resistance mechanisms in M. tuberculosis: an update. Arch Toxicol 2016; 90: 585–1604.
  • Hendrick JP, Hartl F. Functıons of Heat-Shock 1993.
  • Tran TD, Kwon HY, Kim EH, Kim KW, Briles DE, Pyo S, Rhee DK. Decrease in penicillin susceptibility due to heat shock protein ClpL in Streptococcus pneumoniae. Antimicrob Agents Chemother 2011; 55: 2714–2728.
  • Briffotaux J, Liu S, Gicquel B. Genome-wide transcriptional responses of Mycobacterium to antibiotics. Front Microbiol 2019; 10: 1–14.
  • A. Mudaliar AV, Kashyap RS, Purohit HJ, Taori GM, Daginawala HF. Detection of 65 kD heat shock protein in cerebrospinal fluid of tuberculous meningitis patients. BMC Neurol 2006; 6: 1–7.
  • Senna SG, Battilana J, Costa JC, Silva MG, Duarte RS, Fonseca LS, Suffys PN, Bogo MR. Sequencing of hsp65 gene for identification of Mycobacterium species isolated from environmental and clinical sources in Rio de Janeiro, Brazil. J Clin Microbiol 2008; 46: 3822–3825.
  • Nour-Neamatollahi A, Siadat SD, Yari S, Tasbiti AH, Ebrahimzadeh N, Vaziri F, Fateh A, Ghazanfari M, Abdolrahimi F, Pourazar S, Bahrmand A. A new diagnostic tool for rapid and accurate detection of Mycobacterium tuberculosis. Saudi J Biol Sci 2018; 25: 418–425.
  • Onat Akbulut S, Ghorbanpoor H, İpteç BÖ, Butterworth A, Avcıoğlu G, Kozacı LD, Topateş G, Corrigan DK, Avci H, Guzel FD. Impedance testing of porous Si3N4 scaffolds for skeletal implant applications SN Appl Sci 2020; 2: 823-828.
  • Guzel FD, Citak F. Development of an on-chip antibiotic permeability assay with single molecule detection capability. IEEE Trans Nanobioscience 2018; 17: 155–160.
  • Malhotra S, Verma A, Tyagi N, Kumar V. Biosensors: principle, types and applications. Int J Adv Res Innov Ideas Educ 2017; 3: 3639–3644.
  • Baselt DR, Lee GU, Natesan M, Metzger SW, Sheehan PE, Colton RJ. A biosensor based on magnetoresistance technology. Biosens Bioelectron 1998; 13: 731–739.
  • Yang R, Jin J, Chen Y, Shao N, Kang H, Xiao Z, Tang Z, Wu Y, Zhu Z, Tan W. Carbon nanotube-quenched fluorescent oligonucleotides: probes that fluoresce upon hybridization. J Am Chem Soc 2008; 130: 8351–8358.
  • Niu SY, Li QY, Ren R, Zhang SS. Enzyme-enhanced fluorescence detection of DNA on etched optical fibers. Biosens Bioelectron 2009; 24: 2943–2946.
  • Wilson JN, Teo YN, Kool ET. Efficient quenching of oligomeric fluorophores on a DNA backbone. J Am Chem Soc 2007; 129: 15426–15427.
  • Randviir EP, Banks CE. Electrochemical impedance spectroscopy: an overview of bioanalytical applications. Anal Methods 2013; 5: 1098–1115.
  • Blair EO, Corrigan DK. A review of microfabricated electrochemical biosensors for DNA detection. Biosens Bioelectron 2019; 134: 57–67.
  • Liu J, Cao Z, Lu Y. Functional nucleic acid sensors. Chem Rev 2009; 109: 1948–1998.
  • Blair EO, Hannah S, Vezza V, Avcı H, Kocagoz T, Hoskisson PA, Güzel FD, Corrigan DK. Biologically modified microelectrode sensors provide enhanced sensitivity for detection of nucleic acid sequences from Mycobacterium tuberculosis. Sensors and Actuators Reports. Sens. Actuator report 2020; 2: 100008.
  • Huang Y, Bell MC, Suni II. Impedance biosensor for peanut protein Ara h 1. Anal Chem 2008; 80: 9157–9161.
  • PS J, Sutrave DS. A brief study of cyclic voltammetry and electrochemical analysis. Int J ChemTech Res 2018; 11: 77–88.
  • Butterworth A, Blues E, Williamson P, Cardona M, Gray L, Corrigan DK. SAM composition and electrode roughness affect performance of a DNA biosensor for antibiotic resistance. Biosens 2019; 9: 1-12.
  • Bedford E. Gold surface nanostructuring for separation and sensing of biomolecules. Doctoral dissertation 2017.
  • Keighley SD, Li P, Estrela P, Migliorato P. Optimization of DNA immobilization on gold electrodes for label-free detection by electrochemical impedance spectroscopy. Biosens Bioelectronvol 2008; 23: 1291–1297.
  • Guzel FD, Ghorbanpoor H, Dizaji AN, Akcakoca I, Ozturk Y, Kocagoz T, Corrigan DK, Avci H. Biotechnol Appl Biochem 2020; doi:10.1002/bab.2037.
  • Lisdat F, Schäfer D. The use of electrochemical impedance spectroscopy for biosensing. Anal Bioanal Chem 2008; 391: 1555–1567.
  • Park JY, Park SM. DNA hybridization sensors based on electrochemical impedance spectroscopy as a detection tool. Sensors 2009; 9: 9513–9532.
  • Drummond TG, Hill MG, Barton JK. Electrochemical DNA sensors. Nat Biotechnol 2003; l: 1192–1199.
  • Demirbakan B, Sezgintürk MK. A novel immunosensor based on fullerene C60 for electrochemical analysis of heat shock protein 70. J Electroanal Chem 2016; 783: 201–207.
  • Sun B, Cai J, Li W, Gou X, Gou Y, Li D, Hu F. A novel electrochemical immunosensor based on PG for early screening of depression markers-heat shock protein 70. Biosens Bioelectron 2018; 111: 34–40.
  • Aguilar ZP, Fritsch I. Immobilized Enzyme-Linked DNA-Hybridization Assay with Electrochemical Detection for Cryptosporidium p arvum hsp70 mRNA. Anal Chem 2003; 75: 3890–3897.

Yıl 2021, Cilt 22, Sayı 4, 344 - 352, 29.12.2021
https://doi.org/10.18038/estubtda.806222

Öz

Kaynakça

  • Garred P, Brygge K, Sorensen CH, Madsen HO, Thiel S, Svejgaard A. Mycobacteria in water. J Appl Bacteriol 1984; 57: 193–211.
  • Beere HM. “The stress of dying”: The role of heat shock proteins in the regulation of apoptosis. J Cell Sci 2004; 117: 2641–2651.
  • Nguyen L. Antibiotic resistance mechanisms in M. tuberculosis: an update. Arch Toxicol 2016; 90: 585–1604.
  • Hendrick JP, Hartl F. Functıons of Heat-Shock 1993.
  • Tran TD, Kwon HY, Kim EH, Kim KW, Briles DE, Pyo S, Rhee DK. Decrease in penicillin susceptibility due to heat shock protein ClpL in Streptococcus pneumoniae. Antimicrob Agents Chemother 2011; 55: 2714–2728.
  • Briffotaux J, Liu S, Gicquel B. Genome-wide transcriptional responses of Mycobacterium to antibiotics. Front Microbiol 2019; 10: 1–14.
  • A. Mudaliar AV, Kashyap RS, Purohit HJ, Taori GM, Daginawala HF. Detection of 65 kD heat shock protein in cerebrospinal fluid of tuberculous meningitis patients. BMC Neurol 2006; 6: 1–7.
  • Senna SG, Battilana J, Costa JC, Silva MG, Duarte RS, Fonseca LS, Suffys PN, Bogo MR. Sequencing of hsp65 gene for identification of Mycobacterium species isolated from environmental and clinical sources in Rio de Janeiro, Brazil. J Clin Microbiol 2008; 46: 3822–3825.
  • Nour-Neamatollahi A, Siadat SD, Yari S, Tasbiti AH, Ebrahimzadeh N, Vaziri F, Fateh A, Ghazanfari M, Abdolrahimi F, Pourazar S, Bahrmand A. A new diagnostic tool for rapid and accurate detection of Mycobacterium tuberculosis. Saudi J Biol Sci 2018; 25: 418–425.
  • Onat Akbulut S, Ghorbanpoor H, İpteç BÖ, Butterworth A, Avcıoğlu G, Kozacı LD, Topateş G, Corrigan DK, Avci H, Guzel FD. Impedance testing of porous Si3N4 scaffolds for skeletal implant applications SN Appl Sci 2020; 2: 823-828.
  • Guzel FD, Citak F. Development of an on-chip antibiotic permeability assay with single molecule detection capability. IEEE Trans Nanobioscience 2018; 17: 155–160.
  • Malhotra S, Verma A, Tyagi N, Kumar V. Biosensors: principle, types and applications. Int J Adv Res Innov Ideas Educ 2017; 3: 3639–3644.
  • Baselt DR, Lee GU, Natesan M, Metzger SW, Sheehan PE, Colton RJ. A biosensor based on magnetoresistance technology. Biosens Bioelectron 1998; 13: 731–739.
  • Yang R, Jin J, Chen Y, Shao N, Kang H, Xiao Z, Tang Z, Wu Y, Zhu Z, Tan W. Carbon nanotube-quenched fluorescent oligonucleotides: probes that fluoresce upon hybridization. J Am Chem Soc 2008; 130: 8351–8358.
  • Niu SY, Li QY, Ren R, Zhang SS. Enzyme-enhanced fluorescence detection of DNA on etched optical fibers. Biosens Bioelectron 2009; 24: 2943–2946.
  • Wilson JN, Teo YN, Kool ET. Efficient quenching of oligomeric fluorophores on a DNA backbone. J Am Chem Soc 2007; 129: 15426–15427.
  • Randviir EP, Banks CE. Electrochemical impedance spectroscopy: an overview of bioanalytical applications. Anal Methods 2013; 5: 1098–1115.
  • Blair EO, Corrigan DK. A review of microfabricated electrochemical biosensors for DNA detection. Biosens Bioelectron 2019; 134: 57–67.
  • Liu J, Cao Z, Lu Y. Functional nucleic acid sensors. Chem Rev 2009; 109: 1948–1998.
  • Blair EO, Hannah S, Vezza V, Avcı H, Kocagoz T, Hoskisson PA, Güzel FD, Corrigan DK. Biologically modified microelectrode sensors provide enhanced sensitivity for detection of nucleic acid sequences from Mycobacterium tuberculosis. Sensors and Actuators Reports. Sens. Actuator report 2020; 2: 100008.
  • Huang Y, Bell MC, Suni II. Impedance biosensor for peanut protein Ara h 1. Anal Chem 2008; 80: 9157–9161.
  • PS J, Sutrave DS. A brief study of cyclic voltammetry and electrochemical analysis. Int J ChemTech Res 2018; 11: 77–88.
  • Butterworth A, Blues E, Williamson P, Cardona M, Gray L, Corrigan DK. SAM composition and electrode roughness affect performance of a DNA biosensor for antibiotic resistance. Biosens 2019; 9: 1-12.
  • Bedford E. Gold surface nanostructuring for separation and sensing of biomolecules. Doctoral dissertation 2017.
  • Keighley SD, Li P, Estrela P, Migliorato P. Optimization of DNA immobilization on gold electrodes for label-free detection by electrochemical impedance spectroscopy. Biosens Bioelectronvol 2008; 23: 1291–1297.
  • Guzel FD, Ghorbanpoor H, Dizaji AN, Akcakoca I, Ozturk Y, Kocagoz T, Corrigan DK, Avci H. Biotechnol Appl Biochem 2020; doi:10.1002/bab.2037.
  • Lisdat F, Schäfer D. The use of electrochemical impedance spectroscopy for biosensing. Anal Bioanal Chem 2008; 391: 1555–1567.
  • Park JY, Park SM. DNA hybridization sensors based on electrochemical impedance spectroscopy as a detection tool. Sensors 2009; 9: 9513–9532.
  • Drummond TG, Hill MG, Barton JK. Electrochemical DNA sensors. Nat Biotechnol 2003; l: 1192–1199.
  • Demirbakan B, Sezgintürk MK. A novel immunosensor based on fullerene C60 for electrochemical analysis of heat shock protein 70. J Electroanal Chem 2016; 783: 201–207.
  • Sun B, Cai J, Li W, Gou X, Gou Y, Li D, Hu F. A novel electrochemical immunosensor based on PG for early screening of depression markers-heat shock protein 70. Biosens Bioelectron 2018; 111: 34–40.
  • Aguilar ZP, Fritsch I. Immobilized Enzyme-Linked DNA-Hybridization Assay with Electrochemical Detection for Cryptosporidium p arvum hsp70 mRNA. Anal Chem 2003; 75: 3890–3897.

Ayrıntılar

Birincil Dil İngilizce
Konular Mühendislik
Bölüm Makaleler
Yazarlar

Fatma DOĞAN GUZEL (Sorumlu Yazar)
ANKARA YILDIRIM BEYAZIT UNIVERSITY
0000-0001-7200-4615
Türkiye


Iremnur AKCAKOCA Bu kişi benim
ANKARA YILDIRIM BEYAZIT UNIVERSITY
0000-0001-9752-4190
Türkiye


Hamed GHORBANPOOR
Eskisehir Osmangazi University
0000-0002-2665-8172
Türkiye


Araz NOROUZ DİZAJİ Bu kişi benim
ANKARA YILDIRIM BEYAZIT UNIVERSITY
0000-0001-6720-2115
Türkiye


Yasin ÖZTÜRK Bu kişi benim
ANKARA YILDIRIM BEYAZIT UNIVERSITY
0000-0002-9049-0992
Türkiye


Ewen BLAİR Bu kişi benim
University Of Strathclyde
0000-0002-1887-8001
United Kingdom


Tanıl KOCAGOZ Bu kişi benim
ACIBADEM MEHMET ALI AYDINLAR UNIVERSITY
0000-0001-7211-2026
Türkiye


Damion CORRİGAN Bu kişi benim
University Of Strathclyde
0000-0002-4647-7483
United Kingdom


Hüseyin AVCI
ESKİŞEHİR OSMANGAZİ ÜNİVERSİTESİ
0000-0002-2475-1963
Türkiye

Destekleyen Kurum TÜBİTAK
Proje Numarası 217S793
Teşekkür This study was conducted in the frame of Newton Katip Celebi Fund between Turkey and UK and supported by Turkish Scientific and Technological Council (TUBITAK) under the grant number of 217S793. Authors would like to thank I. A. Ince for useful discussions.
Yayımlanma Tarihi 29 Aralık 2021
Yayınlandığı Sayı Yıl 2021, Cilt 22, Sayı 4

Kaynak Göster

Bibtex @araştırma makalesi { estubtda806222, journal = {Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering}, issn = {2667-4211}, address = {btda@anadolu.edu.tr}, publisher = {Eskişehir Teknik Üniversitesi}, year = {2021}, volume = {22}, number = {4}, pages = {344 - 352}, doi = {10.18038/estubtda.806222}, title = {HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES}, key = {cite}, author = {Doğan Guzel, Fatma and Akcakoca, Iremnur and Ghorbanpoor, Hamed and Norouz Dizaji, Araz and Öztürk, Yasin and Blair, Ewen and Kocagoz, Tanıl and Corrigan, Damion and Avcı, Hüseyin} }
APA Doğan Guzel, F. , Akcakoca, I. , Ghorbanpoor, H. , Norouz Dizaji, A. , Öztürk, Y. , Blair, E. , Kocagoz, T. , Corrigan, D. & Avcı, H. (2021). HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES . Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering , 22 (4) , 344-352 . DOI: 10.18038/estubtda.806222
MLA Doğan Guzel, F. , Akcakoca, I. , Ghorbanpoor, H. , Norouz Dizaji, A. , Öztürk, Y. , Blair, E. , Kocagoz, T. , Corrigan, D. , Avcı, H. "HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES" . Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering 22 (2021 ): 344-352 <https://dergipark.org.tr/tr/pub/estubtda/issue/67431/806222>
Chicago Doğan Guzel, F. , Akcakoca, I. , Ghorbanpoor, H. , Norouz Dizaji, A. , Öztürk, Y. , Blair, E. , Kocagoz, T. , Corrigan, D. , Avcı, H. "HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES". Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering 22 (2021 ): 344-352
RIS TY - JOUR T1 - HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES AU - Fatma Doğan Guzel , Iremnur Akcakoca , Hamed Ghorbanpoor , Araz Norouz Dizaji , Yasin Öztürk , Ewen Blair , Tanıl Kocagoz , Damion Corrigan , Hüseyin Avcı Y1 - 2021 PY - 2021 N1 - doi: 10.18038/estubtda.806222 DO - 10.18038/estubtda.806222 T2 - Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering JF - Journal JO - JOR SP - 344 EP - 352 VL - 22 IS - 4 SN - 2667-4211- M3 - doi: 10.18038/estubtda.806222 UR - https://doi.org/10.18038/estubtda.806222 Y2 - 2021 ER -
EndNote %0 Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES %A Fatma Doğan Guzel , Iremnur Akcakoca , Hamed Ghorbanpoor , Araz Norouz Dizaji , Yasin Öztürk , Ewen Blair , Tanıl Kocagoz , Damion Corrigan , Hüseyin Avcı %T HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES %D 2021 %J Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering %P 2667-4211- %V 22 %N 4 %R doi: 10.18038/estubtda.806222 %U 10.18038/estubtda.806222
ISNAD Doğan Guzel, Fatma , Akcakoca, Iremnur , Ghorbanpoor, Hamed , Norouz Dizaji, Araz , Öztürk, Yasin , Blair, Ewen , Kocagoz, Tanıl , Corrigan, Damion , Avcı, Hüseyin . "HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES". Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering 22 / 4 (Aralık 2021): 344-352 . https://doi.org/10.18038/estubtda.806222
AMA Doğan Guzel F. , Akcakoca I. , Ghorbanpoor H. , Norouz Dizaji A. , Öztürk Y. , Blair E. , Kocagoz T. , Corrigan D. , Avcı H. HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES. Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering. 2021; 22(4): 344-352.
Vancouver Doğan Guzel F. , Akcakoca I. , Ghorbanpoor H. , Norouz Dizaji A. , Öztürk Y. , Blair E. , Kocagoz T. , Corrigan D. , Avcı H. HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES. Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering. 2021; 22(4): 344-352.
IEEE F. Doğan Guzel , I. Akcakoca , H. Ghorbanpoor , A. Norouz Dizaji , Y. Öztürk , E. Blair , T. Kocagoz , D. Corrigan ve H. Avcı , "HIGHLY SENSITIVE LABEL-FREE ELECTROCHEMICAL DETECTION OF HEAT SHOCK PROTEIN WITH LOW-COST SCREEN-PRINTED ELECTRODES", Eskişehir Technical University Journal of Science and Technology A - Applied Sciences and Engineering, c. 22, sayı. 4, ss. 344-352, Ara. 2021, doi:10.18038/estubtda.806222