EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA

Muhammet Ceylan; Shang-you Yang; Ramazan Asmatulu

doi:10.20290/estubtdb.658998

EN TR

EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA

Abstract

Polycaprolactone (PCL) nanofiber with constant plasmid DNA addition and with different concentration of gentamicin designed and studied for their effect on cell viability and release rate. PCL nanofibers were fabricated using electrospinning method with plasmid DNA and gentamicin addition. The plasmid DNA used in PCL nanofiber were extracted from E. coli. The scanning electron microscopy (SEM) images show that the nano-scale fiber structures have an average diameter of 113.9 nm. UV microplate reader confirmed the existence of plasmid DNA in the PCL nanofibers. Elisa reader study showed the addition of gentamicin in the fibers. Cell viability tests indicated that PCL nanofibers with 10% gentamicin on a fibroblast cell showed high cell viability, which is related to surface areas and pore size of the electrospun fibers besides to the interaction among gentamicin, plasmid DNA and electrospun fiber matrix.

Keywords

Electrospinning, PCL nanofibers, Wound Dressing, Cell viability

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Details

Primary Language

English

Subjects

Engineering

Journal Section

Research Article

Authors

Muhammet Ceylan ^*
0000-0001-6933-2917
Türkiye

Shang-you Yang This is me
0000-0002-8835-5302
United States

Ramazan Asmatulu
0000-0001-8104-2285
United States

Publication Date

August 31, 2020

Submission Date

December 20, 2019

Acceptance Date

June 30, 2020

Published in Issue

Year 2020 Volume: 8 Number: 2

DOI

https://doi.org/10.20290/estubtdb.658998

IZ

https://izlik.org/JA24YL27AZ

APA

Ceylan, M., Yang, S.- you, & Asmatulu, R. (2020). EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi B - Teorik Bilimler, 8(2), 293-302. https://doi.org/10.20290/estubtdb.658998

AMA

1.Ceylan M, Yang S you, Asmatulu R. EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi B - Teorik Bilimler. 2020;8(2):293-302. doi:10.20290/estubtdb.658998

Chicago

Ceylan, Muhammet, Shang-you Yang, and Ramazan Asmatulu. 2020. “EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi B - Teorik Bilimler 8 (2): 293-302. https://doi.org/10.20290/estubtdb.658998.

EndNote

Ceylan M, Yang S- you, Asmatulu R (August 1, 2020) EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi B - Teorik Bilimler 8 2 293–302.

IEEE

[1]M. Ceylan, S.- you Yang, and R. Asmatulu, “EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA”, Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi B - Teorik Bilimler, vol. 8, no. 2, pp. 293–302, Aug. 2020, doi: 10.20290/estubtdb.658998.

ISNAD

Ceylan, Muhammet - Yang, Shang-you - Asmatulu, Ramazan. “EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA”. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi B - Teorik Bilimler 8/2 (August 1, 2020): 293-302. https://doi.org/10.20290/estubtdb.658998.

JAMA

1.Ceylan M, Yang S- you, Asmatulu R. EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi B - Teorik Bilimler. 2020;8:293–302.

MLA

Ceylan, Muhammet, et al. “EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi B - Teorik Bilimler, vol. 8, no. 2, Aug. 2020, pp. 293-02, doi:10.20290/estubtdb.658998.

Vancouver

1.Muhammet Ceylan, Shang-you Yang, Ramazan Asmatulu. EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi B - Teorik Bilimler. 2020 Aug. 1;8(2):293-302. doi:10.20290/estubtdb.658998

EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA

EFFECTS OF GENTAMICIN LOADED PCL NANOFIBERS TO CELL VIABILITY AND RELEASE RATE OF PLASMID DNA

Abstract

Keywords

GENTAMICIN YÜKLENEN PCL NANOFİBERLERİNİN ÇEŞİTLİLİK HÜCRESİNE ETKİSİ VE PLAZMİD DNA ORANI

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