IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300

Demet Hançer Aydemir

doi:10.18036/estubtdc.1536495

TR EN

IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300

Abstract

Escherichia coli ve Metisiline dirençli Staphylococcus aureus (MRSA) enfeksiyonlarının tedavisi, kullanılan antibiyotiklere karşı artan direnç gelişimi nedeniyle zorlaşmıştır. Günümüzde bu enfeksiyonlarla mücadelede, doğal ve ucuz bitkisel esansiyel yağların ve antibakteriyel aktivitesi bilinen biyoaktif moleküllerinin önemi artmaktadır. Bu çalışmada, bakterilerin direnç geliştiremediği Origanum onites esansiyel yağı (OEO) ve karvakrolün antibiyotiklere alternatif olarak antibakteriyel ve antibiyofilm etkilerinin araştırılması amaçlanmıştır. OEO'nun kimyasal içeriği GC-MS sistemi ile analiz edilmiştir. Antibakteriyel aktivite, disk difüzyon, tüp dilüsyon ve antibakteriyel eğri analizleri ile, antibiyofilm aktivitesi ise kantitatif kristal viyole yöntemi ile analiz edilmiştir. Karvakrol, OEO bileşimindeki majör bileşen olarak tanımlanmıştır. Sonuçlar, OEO ve karvakrolün sırasıyla 100 µg/mL ve 50 µg/mL'lik minimum inhibisyon konsantrasyonu (MİK) ile E. coli ve MRSA'ya karşı antibakteriyel aktivite gösterdiğini gösterdi. Antibakteriyel eğri testi sonuçları, OEO ve karvakrolün E. coli ve MRSA'ya karşı bakterisidal aktivite gösterdiğini gösterdi. OEO ve karvakrolün E. coli ve MRSA'nın biyofilm oluşumunu MİK, 1/2MİK ve 1/4MİK konsantrasyonlarında %15,5-%80,7 aralığında inhibe ettiği görüldü.

Keywords

Escherichia coli, Origanum onites, Antibiofilm activity, Carvacrol, Methicillin-resistant Staphylococcus aureus

IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300

Abstract

The treatment of infections caused by Escherichia coli and Methicillin-resistant Staphylococcus aureus (MRSA) has become difficult due to the increasing development of resistance to the antibiotics used. Nowadays, in the fight against these infections, the importance of natural and inexpensive plant essential oils and their bioactive molecules with known antibacterial activity has been increasing. This study aimed to investigate the antibacterial and antibiofilm effects of Origanum onites essential oil (OEO) and carvacrol, to which bacteria cannot develop resistance, as an alternative to antibiotics. The chemical content of OEO was analyzed by GC-MS system. Antibacterial activity was analyzed by disk diffusion, macro broth dilution, and antibacterial curve assays, and also antibiofilm activity was analyzed by the quantitative crystal violet method. Carvacrol was defined as the major component in the OEO composition. The results showed that OEO and carvacrol exhibited antibacterial activity against E. coli and MRSA with the minimum inhibition concentration (MIC) of 100 µg/mL and 50 µg/mL, respectively. The antibacterial curve assay results showed that OEO and carvacrol exhibited bactericidal activity against E. coli and MRSA. OEO and carvacrol inhibited the biofilm formation of E. coli and MRSA in the range of 15.5%-80.7% at MIC, 1/2MIC, and 1/4MIC concentrations.

Keywords

Escherichia coli, Origanum onites, Antibiofilm activity, Carvacrol, Methicillin-resistant Staphylococcus aureus

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Details

Primary Language

English

Subjects

Bacteriology

Journal Section

Research Article

Authors

Demet Hançer Aydemir ^*
0000-0003-0174-8769
Türkiye

Publication Date

July 25, 2025

Submission Date

August 21, 2024

Acceptance Date

February 26, 2025

Published in Issue

Year 2025 Volume: 14 Number: 2

DOI

https://doi.org/10.18036/estubtdc.1536495

IZ

https://izlik.org/JA46AD54TX

APA

Hançer Aydemir, D. (2025). IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 14(2), 44-58. https://doi.org/10.18036/estubtdc.1536495

AMA

1.Hançer Aydemir D. IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2025;14(2):44-58. doi:10.18036/estubtdc.1536495

Chicago

Hançer Aydemir, Demet. 2025. “IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum Onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia Coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus Aureus (MRSA) ATCC 43300”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 14 (2): 44-58. https://doi.org/10.18036/estubtdc.1536495.

EndNote

Hançer Aydemir D (July 1, 2025) IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 14 2 44–58.

IEEE

[1]D. Hançer Aydemir, “IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300”, Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 14, no. 2, pp. 44–58, July 2025, doi: 10.18036/estubtdc.1536495.

ISNAD

Hançer Aydemir, Demet. “IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum Onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia Coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus Aureus (MRSA) ATCC 43300”. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 14/2 (July 1, 2025): 44-58. https://doi.org/10.18036/estubtdc.1536495.

JAMA

1.Hançer Aydemir D. IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2025;14:44–58.

MLA

Hançer Aydemir, Demet. “IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum Onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia Coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus Aureus (MRSA) ATCC 43300”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 14, no. 2, July 2025, pp. 44-58, doi:10.18036/estubtdc.1536495.

Vancouver

1.Demet Hançer Aydemir. IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2025 Jul. 1;14(2):44-58. doi:10.18036/estubtdc.1536495

IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300

Abstract

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IN-VITRO ANTIBIOFILM ACTIVITY AND GROWTH INHIBITORY EFFECTS OF Origanum onites ESSENTIAL OIL AND CARVACROL AGAINST Escherichia coli ATCC 25922 AND METHICILLIN-RESISTANT Staphylococcus aureus (MRSA) ATCC 43300

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