IN VITRO BIOLOGICAL ACTIVITY EVALUATION OF ETHANOLIC EXTRACT OF MELICA UNIFLORA LEAVES

Yıl 2021, , 17 - 26, 25.01.2021

Elif Çil , Sevda Türkiş , Melek Çol Ayvaz , Ceren Börçek Kasurka

https://doi.org/10.18036/estubtdc.587101

Cited By: 2

Öz

Melica uniflora is part of the omnivore and
herbivore animal diets and there is not enough study about this plant. The aim
of the study is to provide the antimicrobial and antioxidant potential of
ethanol extract of M. uniflora leaves. 
Total phenolic and
flavonoid contents and antioxidant potential based on different methods and
antimicrobial activity of M. uniflora leaves ethanol extract were examined.
Disk diffusion method against to
eight bacteria strains, the Folin-Ciocalteu
method, DPPH free radical scavenging activity and ferric reducing
antioxidant power assays were used. Mean diameters of inhibition zones were found in the range of
14.93 mm to 26.32 mm.
The total phenolic content of ethanol extract of M. uniflora leaves was
determined as 0.466 mg GAE/g. Total antioxidant activity value of the extract was
calculated 4.54 mg AAE/g. IC₅₀
value was calculated 18.798 mg/mL for DPPH free radical scavenging activity.
The FRAP value indicated that the reducing
power of 1 gram of sample was equivalent to 3.33 µmol of trolox. The flavonoid
content of the extract was calculated as
4.44 mgQE/g. According to the obtained results, the analyzed M.
uniflora leaves ethanol extracts demonstrated antioxidant and antimicrobial
activity level can be considered effective. 

Anahtar Kelimeler

Wood melick, forage selection, Yenice Forest, forest health, Nocardia

Destekleyen Kurum

Ordu University

Proje Numarası

Scientific Research Project No: HD 1707

Teşekkür

The authors gratefully acknowledge financial support through and would like to thank the Ordu University (Scientific Research Project No: HD 1707).

MELICA UNIFLORA YAPRAKLARININ ETANOLİK ÖZÜTLERİNİN IN VITRO BİYOLOJİK AKTİVİTESİNİN DEĞERLENDİRİLMESİ

Öz

Melica
uniflora, omnivor ve otobur hayvan diyetlerinin bir
parçasıdır ve bu bitki hakkında yeterli çalışma yoktur. Çalışmanın amacı, M. uniflora yapraklarının etanol
ekstraktının antimikrobiyal ve antioksidan potansiyelini belirlemektir. M. uniflora yapraklarının etanol
özütlerinin antimikrobiyal aktiviteleri ile toplam fenolik ve flavonoid
içerikleri ve antioksidan potansiyeli farklı yöntemlere dayanarak
incelenmiştir. Çalışmada sekiz bakteri suşuyla disk difüzyon metodu,
FolinCiocalteu metodu, DPPH serbest radikal temizleme aktivitesi ve ferrik
indirgeyici antioksidan güç analizleri kullanılmıştır. Ortalama inhibisyon zon
çapları 14.93 mm ile 26.32 mm arasında bulunmuştur. M. uniflora yapraklarının etanol ekstraktının toplam fenolik
içeriği 0.466 mg GAE/g olarak belirlenmiştir. Ekstrenin toplam antioksidan
aktivite değeri 4.54 mg AAE/g olarak hesaplanmıştır. IC₅₀ değeri
DPPH serbest radikal süpürme aktivitesi için 18.798 mg/mL olarak hesaplanmıştır.
FRAP değeri, 1 gram numunenin indirgeme gücünün 3.33 umol troloks'a eşdeğer
olduğunu göstermiştir. Ekstrenin flavonoid içeriği 4.44 mgQE/g olarak
hesaplanmıştır. Elde edilen sonuçlara göre, analiz edilen M. uniflora etanol özütlerinin antioksidan ve antimikrobiyal
aktivite gösterdiği tespit edilmiştir.

Anahtar Kelimeler

Seyrek inci otu, yem seçimi, Yenice Ormanı, orman sağlığı, Nocardia

Proje Numarası

Scientific Research Project No: HD 1707

Kaynakça

• FAO. Forest health http://www.fao.org/forestry/pests/en/ (Last accessed: January 2019)
• Trumbore S, Brando P, Hartmann H. Forest health and global change. Science. 2015; 349(6250): 814-8.
• Ferretti M. Forest health assessment and monitoring–issues for consideration. Environmental monitoring and assessment. 1997; 48(1): 45-72.
• Parker KL, Barboza PS, Gillingham MP. Nutrition integrates environmental responses of ungulates. Functional ecology. 2009; 23(1): 57-69.
• Bastin L, Thomas CD. The distribution of plant species in urban vegetation fragments. Landscape Ecology. 1999; 14(5): 493-507.
• Moen J, Gardfjell H, Oksanen L, Ericson L, Ekerholm P. Grazing by food-limited microtine rodents on a productive experimental plant community: does the" green desert" exist? Oikos. 1993; 68: 401-13.
• Davis PH. Flora of Turkey and the East Aegean Islands1970.
• Turkis S, Elmas E. Yenice Ormanlarının Korunan Bitkileri (Çitdere ve Kavaklı Tabiatı Koruma Alanları) 2016.
• Wandscheer CB, Duque JE, da Silva MA, Fukuyama Y, Wohlke JL, Adelmann J, et al. Larvicidal action of ethanolic extracts from fruit endocarps of Melia azedarach and Azadirachta indica against the dengue mosquito Aedes aegypti. Toxicon. 2004; 44(8): 829-35.
• CLSI. Performance Standards for Antimicrobial Susceptibility Testing, 28th Edition https://clsi.org/standards/products/microbiology/documents/m100/
• CLSI. Performance Standards for Antimicrobial Disk Susceptibility Tests, 13th Edition https://clsi.org/standards/products/microbiology/documents/m02/
• Singleton VL, Rossi JA. Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents. American journal of Enology and Viticulture. 1965; 16(3): 144-58.
• Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardization of propolis extract and identification of principal constituents. Journal de pharmacie de Belgique. 1994;49(6):462-8.
• Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Analytical biochemistry. 1999; 269(2): 337-41.
• Sánchez‐Moreno C, Larrauri JA, Saura‐Calixto F. A procedure to measure the antiradical efficiency of polyphenols. Journal of the Science of Food and Agriculture. 1998; 76(2): 270-6.
• Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical biochemistry. 1996; 239(1): 70-6.
• Türkiş S, Elmas E. Effect of environmental factors on species diversity of the Yenice Hot Spot Forests in Turkey. Journal of Forestry Research. 2018; 29(6): 1719-30.
• Krishnan S, Chinnadurai GS, Perumal P. Polyhydroxybutyrate by Streptomyces sp.: Production and characterization. International journal of biological macromolecules. 2017; 104: 1165-71.
• Abd-Elnaby HM, Abo-Elala GM, Abdel-Raouf UM, Hamed MM. Distribution and Characterization of Actinomycetes in Suez Bay Sediments, Egypt. Asian Journal of Applied Sciences (ISSN: 2321–0893). 2016; 4(01).
• Hashemi-Shahraki A, Bostanabad SZ, Heidarieh P, Sheikhi N, Biranvand M, Alavi SM, et al. Species spectrum of Nocardia spp. isolated from suspected tuberculosis patients. Health. 2015; 7(07): 847.
• Parmar RS, Singh C, Kumar A. Characterization And Antagonistic Potential Of Soil Actinomycetes Against Pathogens Of Human Mycosis. Octa Journal of Environmental Research. 2016; 4(4): 299-306.
• Henderson NM, Sutherland RK. Nocardia and Actinomyces. Medicine. 2017.
• Badridze G, Kacharava N, Chkhubianishvili E, Rapava L, Kikvidze M, Chigladze L, et al. Content of antioxidants in leaves of some plants of Tbilisi environs. Bull Georg Natl Acad Sci. 2013; 7(3): 105-11.
• Spitaler R, Schlorhaufer PD, Ellmerer EP, Merfort I, Bortenschlager S, Stuppner H, et al. Altitudinal variation of secondary metabolite profiles in flowering heads of Arnica montana cv. ARBO. Phytochemistry. 2006; 67(4): 409-17.
• Spitaler R, Winkler A, Lins I, Yanar S, Stuppner H, Zidorn C. Altitudinal variation of phenolic contents in flowering heads of Arnica montana cv. ARBO: a 3-year comparison. Journal of chemical ecology. 2008; 34(3): 369-75.
• Zidorn C. Altitudinal variation of secondary metabolites in flowering heads of the Asteraceae: trends and causes. Phytochemistry reviews. 2010; 9(2): 197-203.
• Orhan DD, Özçelik B, Hoşbaş S, Vural M. Assessment of antioxidant, antibacterial, antimycobacterial, and antifungal activities of some plants used as folk remedies in Turkey against dermatophytes and yeast-like fungi. Turkish Journal of Biology. 2012; 36(6): 672-86.
• Jakovljević VD, Milićević JM, Ðelić GT, Vrvić MM. Antioxidant activity of Ruscus species from Serbia: Potential new sources of natural antioxidants. Hemijska industrija. 2016;70(1):99-106.
• Ward H. Marshall: Grasses 2018. 136 p.
• Toïgo C, Gaillard JM, Van Laere G, Hewison M, Morellet N. How does environmental variation influence body mass, body size, and body condition? Roe deer as a case study. Ecography. 2006; 29(3): 301-8.
• Can ÖE, Togan İ. Camera trapping of large mammals in Yenice Forest, Turkey: local information versus camera traps. Oryx. 2009; 43(3): 427-30.
• Campana C, Gauvin S, Ponge J-F. Influence of ground cover on earthworm communities in an unmanaged beech forest: linear gradient studies. European Journal of Soil Biology. 2002; 38(2): 213-24.
• Korboulewsky N, Perez G, Chauvat M. How tree diversity affects soil fauna diversity: A review. Soil Biology and Biochemistry. 2016; 94: 94-106.
• Judis J. Studies on the mechanism of action of phenolic disinfectants II. Patterns of release of radioactivity from Escherichia coli labeled by growth on various compounds. Journal of pharmaceutical sciences. 1963; 52(2): 126-31.
• Juven B, Henis Y, Jacoby B. Studies on the mechanism of the antimicrobial action of oleuropein. Journal of Applied Bacteriology. 1972; 35(4): 559-67.