MELICA UNIFLORA YAPRAKLARININ ETANOLİK ÖZÜTLERİNİN IN VITRO BİYOLOJİK AKTİVİTESİNİN DEĞERLENDİRİLMESİ

Öz

Melica uniflora, omnivor ve otobur hayvan diyetlerinin bir parçasıdır ve bu bitki hakkında yeterli çalışma yoktur. Çalışmanın amacı, M. uniflora yapraklarının etanol ekstraktının antimikrobiyal ve antioksidan potansiyelini belirlemektir. M. uniflora yapraklarının etanol özütlerinin antimikrobiyal aktiviteleri ile toplam fenolik ve flavonoid içerikleri ve antioksidan potansiyeli farklı yöntemlere dayanarak incelenmiştir. Çalışmada sekiz bakteri suşuyla disk difüzyon metodu, FolinCiocalteu metodu, DPPH serbest radikal temizleme aktivitesi ve ferrik indirgeyici antioksidan güç analizleri kullanılmıştır. Ortalama inhibisyon zon çapları 14.93 mm ile 26.32 mm arasında bulunmuştur. M. uniflora yapraklarının etanol ekstraktının toplam fenolik içeriği 0.466 mg GAE/g olarak belirlenmiştir. Ekstrenin toplam antioksidan aktivite değeri 4.54 mg AAE/g olarak hesaplanmıştır. IC₅₀ değeri DPPH serbest radikal süpürme aktivitesi için 18.798 mg/mL olarak hesaplanmıştır. FRAP değeri, 1 gram numunenin indirgeme gücünün 3.33 umol troloks'a eşdeğer olduğunu göstermiştir. Ekstrenin flavonoid içeriği 4.44 mgQE/g olarak hesaplanmıştır. Elde edilen sonuçlara göre, analiz edilen M. uniflora etanol özütlerinin antioksidan ve antimikrobiyal aktivite gösterdiği tespit edilmiştir.

Anahtar Kelimeler

• Seyrek inci otu
• yem seçimi
• Yenice Ormanı
• orman sağlığı
• Nocardia

IN VITRO BIOLOGICAL ACTIVITY EVALUATION OF ETHANOLIC EXTRACT OF MELICA UNIFLORA LEAVES

Öz

Melica uniflora is part of the omnivore and herbivore animal diets and there is not enough study about this plant. The aim of the study is to provide the antimicrobial and antioxidant potential of ethanol extract of M. uniflora leaves.  Total phenolic and flavonoid contents and antioxidant potential based on different methods and antimicrobial activity of M. uniflora leaves ethanol extract were examined. Disk diffusion method against to eight bacteria strains, the Folin-Ciocalteu method, DPPH free radical scavenging activity and ferric reducing antioxidant power assays were used. Mean diameters of inhibition zones were found in the range of 14.93 mm to 26.32 mm. The total phenolic content of ethanol extract of M. uniflora leaves was determined as 0.466 mg GAE/g. Total antioxidant activity value of the extract was calculated 4.54 mg AAE/g. IC₅₀ value was calculated 18.798 mg/mL for DPPH free radical scavenging activity. The FRAP value indicated that the reducing power of 1 gram of sample was equivalent to 3.33 µmol of trolox. The flavonoid content of the extract was calculated as 4.44 mgQE/g. According to the obtained results, the analyzed M. uniflora leaves ethanol extracts demonstrated antioxidant and antimicrobial activity level can be considered effective. 

Anahtar Kelimeler

• Wood melick
• forage selection
• Yenice Forest
• forest health
• Nocardia

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