DOĞU AKDENİZ SEDİMANLARINDAN İZOLE EDİLEN BAKTERİLERİN FİLOGENETİK ÇEŞİTLİLİĞİ VE ANTİBİYOTİK DİRENÇLİLİĞİ

Öz

Doğu Akdeniz'in sığ ve derin bölgelerindeki sedimanlardan izole edilen bakterilerin filogenetik çeşitliliği ve antibiyotik duyarlılığı çalışılmıştır. Dünyadaki en oligotrofik ortamlardan biri olan çalışma alanından izole edilen 153 suşun Firmicutes ve Gammaproteobacteria taksonlarına ait oldukları bulunmuştur. 16S rRNA gen dizisi analizi ile belirlendiği üzere, her sediman örneğinde Bacillus en sık görülen cins olmuştur. Bu çalışmada 10 farklı bakteri ailesinden çok çeşitli türler elde edilmiş, özellikle toplam izolatların yüzde 12’sini yeni türlerin oluşturduğu görülmüştür. En yüksek antibiyotik direncinin derin havzalara kıyasla kıyı sedimanlarda olduğu gösterilmiştir. Çevresel parametrelerin bakteri toplulukları üzerinde etkisinin olduğu belirtilmekle birlikte Doğu Akdeniz'in derin havzalarına kıyasla özellikle Kuzey Ege Denizi'ndeki daha sığ bölgeler için yüksek taksonlarda daha fazla filogenetik çeşitliliğin olduğu ortaya konmuştur.

Anahtar Kelimeler

• Bakteri filogenetiği,16S rRNA genleri,Antibiyotik duyarlılık,Akdeniz

PHYLOGENETIC DIVERSITY AND ANTIBIOTIC RESISTANCE OF BACTERIA ISOLATED FROM SEDIMENTS OF EASTERN MEDITERRANEAN SEA

Öz

Phylogenetic diversity and antibiotic susceptibility of isolated bacteria from sediments in shallow and deep regions of Eastern Mediterranean Sea were studied. 153 strains isolated from the study area which is one of the most oligotrophic environments in the world were found as affiliated to the Firmicutes and Gammaproteobacteria. As determined by 16S rRNA gene sequence analysis, the Bacillus the most frequent genus was dominant in each sediment samples. The present study provided highly diverse bacterial strains of ten different families especially with probability of representing new taxa as twelve percent of total isolates. The highest antibiotic resistance was obtained from coastal sediments compared to deep basins. The influence of environmental parameters was observed as the variability of bacterial community composition such that phylogenetic diversity was much higher in higher taxa for shallower regions, especially in North Aegean Sea as compared to deep basins of Eastern Mediterranean Sea.

Anahtar Kelimeler

• Bacterial phylogeny,16S rRNA genes,Antibiotic susceptibility,Mediterranean Sea

Kaynakça

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