THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR

Gökhan Duruksu

doi:10.18036/estubtdc.598774

EN TR

THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR

Abstract

ABSTRACT

Packed-bed bioreactors provide larger surface area to volume ratio compared to the static culture on flasks. Therefore, these systems offer ideal production environment for large-scale culture of mesenchymal stem cells (MSCs), but the effect of fluid dynamics on the cell-behavior of MSCs is not fully elucidated. In this study, packed-bed perfusion reactor loaded with different size of polymethyl methacrylate carriers was used to apply different rates of shear stress and drug forces at constant flow rate. The cell viability, cell-expansion, apoptosis and protein secretion levels were analyzed for both unmodified and Vascular Endothelial Growth Factor-positive (VEGF⁺) MSCs. The superficial stress was estimated to between 0.21-0.25 N/m². The results showed that the shear stress reduced the VEGF secretion, and Caspase-3 was activated at high drag force, which cause the reduction of the cell numbers in the bioreactor. The reduction of cytoskeletal actin structures seemed to play the central role in this adverse effect of the non-planar shear stress. The expression reduction of VEGF might also have critical impacts on the tissue engineering applications, in which the formation of vascular construct is essential.

Keywords

Bioreactor,Mesenchymal stem cells,Packed bed column,Protein secretion,Vascular endothelial growth factor

THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR

Abstract

ABSTRACT

Packed-bed bioreactors provide larger surface area to volume ratio compared to the static culture on flasks. Therefore, these systems offer ideal production environment for large-scale culture of mesenchymal stem cells (MSCs), but the effect of fluid dynamics on the cell-behavior of MSCs is not fully elucidated. In this study, packed-bed perfusion reactor loaded with different size of polymethyl methacrylate carriers was used to apply different rates of shear stress and drug forces at constant flow rate. The cell viability, cell-expansion, apoptosis and protein secretion levels were analyzed for both unmodified and Vascular Endothelial Growth Factor-positive (VEGF⁺) MSCs. The superficial stress was estimated to between 0.21-0.25 N/m². The results showed that the shear stress reduced the VEGF secretion, and Caspase-3 was activated at high drag force, which cause the reduction of the cell numbers in the bioreactor. The reduction of cytoskeletal actin structures seemed to play the central role in this adverse effect of the non-planar shear stress. The expression reduction of VEGF might also have critical impacts on the tissue engineering applications, in which the formation of vascular construct is essential.

Keywords

Bioreactor,Mesenchymal stem cells,Packed bed column,Protein secretion,Vascular endothelial growth factor

References

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Details

Primary Language

English

Subjects

Structural Biology

Journal Section

Research Article

Authors

Gökhan Duruksu ^*
0000-0002-3830-2384
Türkiye

Publication Date

July 31, 2019

Submission Date

August 7, 2018

Acceptance Date

April 9, 2019

Published in Issue

Year 2019 Volume: 8 Number: 2

DOI

https://doi.org/10.18036/estubtdc.598774

IZ

https://izlik.org/JA78RY97HY

APA

Duruksu, G. (2019). THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, 8(2), 179-190. https://doi.org/10.18036/estubtdc.598774

AMA

1.Duruksu G. THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2019;8(2):179-190. doi:10.18036/estubtdc.598774

Chicago

Duruksu, Gökhan. 2019. “THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8 (2): 179-90. https://doi.org/10.18036/estubtdc.598774.

EndNote

Duruksu G (July 1, 2019) THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8 2 179–190.

IEEE

[1]G. Duruksu, “THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR”, Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 8, no. 2, pp. 179–190, July 2019, doi: 10.18036/estubtdc.598774.

ISNAD

Duruksu, Gökhan. “THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR”. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji 8/2 (July 1, 2019): 179-190. https://doi.org/10.18036/estubtdc.598774.

JAMA

1.Duruksu G. THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2019;8:179–190.

MLA

Duruksu, Gökhan. “THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR”. Eskişehir Teknik Üniversitesi Bilim Ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji, vol. 8, no. 2, July 2019, pp. 179-90, doi:10.18036/estubtdc.598774.

Vancouver

1.Gökhan Duruksu. THE EFFECT OF DRAG FORCE AND FLOW RATE ON MESENCHYMAL STEM CELLS IN PACKED-BED PERFUSION BIOREACTOR. Eskişehir Teknik Üniversitesi Bilim ve Teknoloji Dergisi - C Yaşam Bilimleri Ve Biyoteknoloji. 2019 Jul. 1;8(2):179-90. doi:10.18036/estubtdc.598774

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